The effect of chronic partial bladder outlet obstruction on corpus cavernosum smooth muscle and Rho-kinase in rabbits

2008 ◽  
Vol 27 (8) ◽  
pp. 826-831 ◽  
Author(s):  
Wei-Yu Lin ◽  
Anita Mannikarottu ◽  
Paul Chichester ◽  
Paul Neuman ◽  
Arnold Johnson ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Bladder Outlet Obstruction ◽  
Corpus Cavernosum ◽  
Rho Kinase ◽  
Outlet Obstruction ◽  
Partial Bladder Outlet Obstruction

Increased corpus cavernosum smooth muscle tone associated with partial bladder outlet obstruction is mediated via Rho-kinase

2005 ◽  
Vol 289 (4) ◽  
pp. R1124-R1130 ◽  
Author(s):  
Shaohua Chang ◽  
Joseph A. Hypolite ◽  
Stephen A. Zderic ◽  
Alan J. Wein ◽  
Samuel Chacko ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Bladder Outlet Obstruction ◽  
Rabbit Model ◽  
Corpus Cavernosum ◽  
Rho Kinase ◽  
Outlet Obstruction ◽  
Smooth Muscle Myosin ◽  
Partial Bladder Outlet Obstruction ◽  
Kinase Expression ◽  
Muscle Myosin

Numerous studies have now demonstrated that lower urinary tract symptoms (LUTS) are associated with erectile dysfunction (ED) in men independent of age or general health. Because one-third of men over the age of 50 will develop LUTS and a recent study showed ED in 62% of patients presenting for LUTS, the importance of determining the mechanistic link between these two pathologies is clear. Using a rabbit model of partial bladder outlet obstruction (PBOO), a primary cause of LUTS, we have identified an increased basal corpus cavernosum smooth muscle (CCSM) tone associated with an elevated level of smooth muscle myosin (SMM) phosphorylation in PBOO compared with sham-operated control rabbits (sham). Results from in vitro kinase and phosphatase assays using purified smooth muscle myosin showed increased kinase and decreased phosphatase activities in cellular extracts from corpora cavernosa isolated from PBOO compared with sham rabbits. Increased Rho-kinase expression in the CCSM of PBOO rabbits was suggested by the observations that Rho-kinase inhibitors attenuated the increased kinase activity and were less effective in relaxing CCSM strips from PBOO vs. sham rabbits. This hypothesis was then confirmed by RT-PCR and Western blotting, which demonstrated increased expression of both isoforms of Rho-kinase (ROKα and ROKβ). Increased SMM basal phosphorylation (necessary for SM contraction) in the CCSM of PBOO rabbits, mediated via an increase in Rho-kinase expression/activity, would be expected to make the CCSM more difficult to relax (necessary for erection), which suggests that the RhoA/Rho-kinase pathway as being involved in the mechanism for LUTS-associated ED.

CHANGES IN THE CORPUS CAVERNOSUM SMOOTH MUSCLE RELATED TO REVERSAL OF PARTIAL BLADDER OUTLET OBSTRUCTION IN RABBITS

2008 ◽  
Vol 179 (4S) ◽  
pp. 278-278
Author(s):  
Wei-Yu Lin ◽  
Anita Mannikarottu ◽  
Ahmet Guven ◽  
Yung-Shun Juan ◽  
Barry A Kogan ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Bladder Outlet Obstruction ◽  
Corpus Cavernosum ◽  
Outlet Obstruction ◽  
Partial Bladder Outlet Obstruction

Enhanced Force Generation by Corpus Cavernosum Smooth Muscle in Rabbits With Partial Bladder Outlet Obstruction

2002 ◽  
pp. 2636-2644 ◽  
Author(s):  
SHAOHUA CHANG ◽  
JOSEPH A. HYPOLITE ◽  
STEPHEN A. ZDERIC ◽  
ALAN J. WEIN ◽  
SAMUEL CHACKO ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Bladder Outlet Obstruction ◽  
Corpus Cavernosum ◽  
Outlet Obstruction ◽  
Force Generation ◽  
Partial Bladder Outlet Obstruction

Enhanced Force Generation by Corpus Cavernosum Smooth Muscle in Rabbits With Partial Bladder Outlet Obstruction

2002 ◽  
Vol 167 (6) ◽  
pp. 2636-2644 ◽  
Author(s):  
SHAOHUA CHANG ◽  
JOSEPH A. HYPOLITE ◽  
STEPHEN A. ZDERIC ◽  
ALAN J. WEIN ◽  
SAMUEL CHACKO ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Bladder Outlet Obstruction ◽  
Corpus Cavernosum ◽  
Outlet Obstruction ◽  
Force Generation ◽  
Partial Bladder Outlet Obstruction
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