Rapid molecular determination of sex for western North American chub ( Gila spp.)

Author(s):  
Steven M. Mussmann ◽  
Melody J. Saltzgiver ◽  
Thomas A. Delomas ◽  
Matthew R. Campbell ◽  
Tracy A. Diver ◽  
...  
Parasitology ◽  
2004 ◽  
Vol 130 (2) ◽  
pp. 239-246 ◽  
Author(s):  
J.-Y. CHUNG ◽  
W.-G. KHO ◽  
S.-Y. HWANG ◽  
E.-Y. JE ◽  
Y.-T. CHUNG ◽  
...  

Acephalic cysticercus (Ac), a rarely developed multilobulated and nonencysted form of larval Taenia, causes hydrocephalus or adhesive arachnoiditis in the ventricles and subarachnoidal space that often lead to fatal outcome in affected patients. Ac has been proposed to originate from T. solium on the basis of morphological features, while no molecular data supporting the presumption have been available. In the present study, we investigated the immunological properties as well as molecular characteristics of Ac that was obtained surgically from 6 patients. Immunoblotting of the cyst fluid from Ac samples demonstrated the constitutive expression of a T. solium metacestode (TsM) 10 kDa protein. Specific antibodies against the truncated 10 kDa protein, which appears to be species specific for TsM cysticercosis, were detected in both serum and cerebrospinal fluid samples of Ac patients. Nucleotide sequences of mitochondrial cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit 1 (ND1) genes of Ac were almost identical to those of T. solium but differed substantially from those of the other Taenia species. In phylogenetic analysis, Ac clustered with T. solium in a well-supported clade. Our results strongly suggest that Ac may have originated from T. solium.


Amongst the Fellows elected to the Royal Society in 1941 were W. T. Astbury for his studies using X-ray analysis to study the structures of natural fibres, and amongst the Foreign Members elected that year was Ross G. Harrison for his contributions to embryology. Astbury and Harrison were very different in temperament, and worked in very different fields on either side of the Atlantic, yet they were united in their approach to the study of biological phenomena. Both Astbury and Harrison believed that the organization and form of biological materials whether wool fibres or the limb-bud in an amphibian embryo depended on molecular structure and pattern. Moreover both were concerned with dynamic aspects of form; Astbury’s greatest achievement was to demonstrate the dynamic, reversible folding and stretching of proteins in the k-m-e-f group, and Harrison looked to changing molecular patterns to account for changing symmetries in the developing embryo. It was this common approach that brought them together and led to Harrison spending a brief month in Leeds where they and K. M. Rudall performed what have been described as ‘truly progressive experiments in molecular biology’. I believe this short series of experiments illuminates the character and work of both Harrison and Astbury and illustrates the difficulties, practical and conceptual, in carrying out ‘progressive experiments’. I shall begin by reviewing briefly the embryological background of the time before going on to discuss in detail the approaches of Harrison and Astbury to their work and the outcome of their collaboration.


1963 ◽  
Vol 41 (9) ◽  
pp. 1331-1346 ◽  
Author(s):  
Aino Henssen

The systematic position of the genus Massalongia and the closely related genera Koerberia, Vestergrenopsis, and Placynthium in the family Peltigeraceae including lichens with hemiangiocarpic apothecia is discussed. The ontogeny of a hemiangiocarpic apothecium is described briefly. A key for the determination of the genera is provided.A general survey is given for the morphology and anatomy of the genus Massalongia. The two species, M. carnosa and M. microphylliza, are described in detail. The new combination M. microphylliza is made.


2015 ◽  
Author(s):  
S. F. F. Santos ◽  
C. A. Wegermann ◽  
M. S. Monsalve ◽  
C. A. Simone ◽  
A. M. C. Ferreira ◽  
...  

Geology ◽  
1999 ◽  
Vol 27 (4) ◽  
pp. 299 ◽  
Author(s):  
Kyle Antonelis ◽  
Daniel J. Johnson ◽  
M. Meghan Miller ◽  
Randy Palmer

2013 ◽  
Vol 47 (2) ◽  
pp. 108-116 ◽  
Author(s):  
Al Harmata ◽  
George Montopoli

1970 ◽  
Vol 53 (3) ◽  
pp. 539-542
Author(s):  
R W Henningson

Abstract A 1968 North American authentic milk sample survey determined that the mean freezing point value of milk is –0.5404°C. Statistical concepts permit the calculation of a value, –0.525°C, 2.326 standard deviations from a mean with 95% confidence that 99% of all subsequent observations will be below the value. Based on this survey, it was recommended that the Interpretation of the freezing point value of milk be made a part of the official final action thermistor cryoscopic method for the determination of the freezing point value of milk, and include the following: an upper limit for the freezing point value of milk, an official definition of an authentic milk sample, and a logical procedure for the confirmation of added water. A copy of the report and an explanatory letter were sent to approximately 100 North American regulatory agencies. Reports were received from 49 regulatory agencies with 39 favoring the recommendations, 6 opposing the recommendations, and 4 having no opinion. It is recommended that the Interpretation of the freezing point of milk be included in both the thermistor cryoscopic and the Hortvet methods.


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