scholarly journals In vivo characterization of skeletal muscle function in nebulin‐deficient mice

2020 ◽  
Vol 61 (3) ◽  
pp. 416-424
Author(s):  
Charlotte Gineste ◽  
Augustin C. Ogier ◽  
Isabelle Varlet ◽  
Zaynab Hourani ◽  
Monique Bernard ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Function ◽  
Skeletal Muscle Function ◽  
In Vivo Characterization ◽  
Deficient Mice

Effects of In Vivo Doxorubicin Treatment on Skeletal Muscle Function

2011 ◽  
Vol 43 (Suppl 1) ◽  
pp. 903
Author(s):  
David S. Hydock ◽  
Chia-Ying Lien ◽  
Brock T. Jensen ◽  
Traci L. Parry ◽  
Carole M. Schneider ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Function ◽  
Skeletal Muscle Function ◽  
Doxorubicin Treatment

scholarly journals In vivo Skeletal Muscle Function and Doxorubicin Treatment in the Rat

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
David Hydock ◽  
Asma Omar ◽  
Eric Bredahl ◽  
Colin Quinn
Keyword(s):  
Skeletal Muscle ◽  
Muscle Function ◽  
Skeletal Muscle Function ◽  
Doxorubicin Treatment

scholarly journals The Influence of Supplemental Dietary Linoleic Acid on Skeletal Muscle Contractile Function in a Rodent Model of Barth Syndrome

2021 ◽  
Vol 12 ◽  
Author(s):  
Mario Elkes ◽  
Martin Andonovski ◽  
Daislyn Vidal ◽  
Madison Farago ◽  
Ryan Modafferi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Linoleic Acid ◽  
Muscle Function ◽  
Barth Syndrome ◽  
Dietary Linoleic Acid ◽  
Skeletal Muscle Function ◽  
Contractile Phenotype ◽  
Muscle Contractile

Barth syndrome is a rare and incurable X-linked (male-specific) genetic disease that affects the protein tafazzin (Taz). Taz is an important enzyme responsible for synthesizing biologically relevant cardiolipin (for heart and skeletal muscle, cardiolipin rich in linoleic acid), a critical phospholipid of mitochondrial form and function. Mutations to Taz cause dysfunctional mitochondria, resulting in exercise intolerance due to skeletal muscle weakness. To date, there has been limited research on improving skeletal muscle function, with interventions focused on endurance and resistance exercise. Previous cell culture research has shown therapeutic potential for the addition of exogenous linoleic acid in improving Taz-deficient mitochondrial function but has not been examined in vivo. The purpose of this study was to examine the influence of supplemental dietary linoleic acid on skeletal muscle function in a rodent model of Barth syndrome, the inducible Taz knockdown (TazKD) mouse. One of the main findings was that TazKD soleus demonstrated an impaired contractile phenotype (slower force development and rates of relaxation) in vitro compared to their WT littermates. Interestingly, this impaired contractile phenotype seen in vitro did not translate to altered muscle function in vivo at the whole-body level. Also, supplemental linoleic acid attenuated, to some degree, in vitro impaired contractile phenotype in TazKD soleus, and these findings appear to be partially mediated by improvements in cardiolipin content and resulting mitochondrial supercomplex formation. Future research will further examine alternative mechanisms of dietary supplemental LA on improving skeletal muscle contractile dysfunction in TazKD mice.

scholarly journals Hindlimb skeletal muscle function in myostatin-deficient mice

2010 ◽  
Vol 43 (1) ◽  
pp. 49-57 ◽  
Author(s):  
Bettina A. Gentry ◽  
J. Andries Ferreira ◽  
Charlotte L. Phillips ◽  
Marybeth Brown
Keyword(s):  
Skeletal Muscle ◽  
Muscle Function ◽  
Skeletal Muscle Function ◽  
Deficient Mice

The Versatility of the Pump-Perfused Rat Hindlimb Preparation: Examples Relating to Skeletal Muscle Function and Energy Metabolism

2005 ◽  
Vol 30 (5) ◽  
pp. 576-590 ◽  
Author(s):  
David J. Baker ◽  
Russell T. Hepple
Keyword(s):  
Skeletal Muscle ◽  
Energy Metabolism ◽  
Muscle Function ◽  
Exercise Physiology ◽  
Muscle Metabolism ◽  
Perfusion Medium ◽  
Skeletal Muscle Function ◽  
And Control ◽  
Fast Freezing

The pump-perfused rat hindlimb model, in various forms, has been in use for several decades. There are many applications for this model, owing to the ability to control the content and rate of perfusion. In the context of exercise physiology this model has been put to particularly good use. In this report we summarize some of the central surgical differences between different versions of the pump-perfused rat hindlimb model, including the double hindlimb + trunk, double hindlimb alone, single hindlimb, and distal hindlimb-alone models. We also summarize specific elements of the perfusion medium and measurement of force used in our lab during assessment of muscle metabolic and contractile responses, and illustrate some of the differences from the in vivo condition that merit consideration. We then provide specific examples of how the single pump-perfused hindlimb and distal hindlimb-alone versions of this model have been used to study muscle function and energy metabolism. In this context we show how this model can be used to permit the experimenter to manipulate and control the rate of O2delivery and to add specific compounds that inhibit a particular aspect of muscle metabolism, such that in combination with measurements of the flux of specific substances across the muscle and/or fast-freezing of muscle after contractions, more can be understood about the metabolic state of the contracting muscles. Key words: aerobic metabolism, mitochondria, aging, adaptation

scholarly journals Rapamycin Reverses Elevated mTORC1 Signaling in Lamin A/C-Deficient Mice, Rescues Cardiac and Skeletal Muscle Function, and Extends Survival

2012 ◽  
Vol 4 (144) ◽  
pp. 144ra103-144ra103 ◽  
Author(s):  
F. J. Ramos ◽  
S. C. Chen ◽  
M. G. Garelick ◽  
D.-F. Dai ◽  
C.-Y. Liao ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Function ◽  
Lamin A ◽  
Skeletal Muscle Function ◽  
Mtorc1 Signaling ◽  
Deficient Mice
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