scholarly journals Regeneration of nerve crush injury using adipose-derived stem cells: A multimodal comparison

2018 ◽  
Vol 58 (4) ◽  
pp. 566-572 ◽  
Author(s):  
Mathias Tremp ◽  
Lima Sprenger ◽  
Lucas Degrugillier ◽  
Dirk Johannes Schaefer ◽  
Srinivas Madduri ◽  
...  
Keyword(s):  
Stem Cells ◽  
Crush Injury ◽  
Adipose Derived Stem Cells ◽  
Nerve Crush ◽  
Nerve Crush Injury

scholarly journals Enhanced Nerve Regeneration by Exosomes Secreted by Adipose-Derived Stem Cells with or without FK506 Stimulation

2021 ◽  
Vol 22 (16) ◽  
pp. 8545
Author(s):  
Cheng-Shyuan Rau ◽  
Pao-Jen Kuo ◽  
Shao-Chun Wu ◽  
Lien-Hung Huang ◽  
Tsu-Hsiang Lu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Nerve Regeneration ◽  
Histone Deacetylases ◽  
Immunosuppressive Drug ◽  
Crush Injury ◽  
Sciatic Nerve Crush ◽  
Adipose Derived Stem Cells ◽  
Absolute Quantitation ◽  
Nerve Crush ◽  
Nerve Crush Injury

Exosomes secreted by adipose-derived stem cells (ADSC-exo) reportedly improve nerve regeneration after peripheral nerve injury. Herein, we investigated whether pretreatment of ADSCs with FK506, an immunosuppressive drug that enhances nerve regeneration, could secret exosomes (ADSC-F-exo) that further augment nerve regeneration. Designed exosomes were topically applied to injured nerve in a mouse model of sciatic nerve crush injury to assess the nerve regeneration efficacy. Outcomes were determined by histomorphometric analysis of semi-thin nerve sections stained with toluidine blue, mouse neurogenesis PCR array, and neurotrophin expression in distal nerve segments. Isobaric tags for relative and absolute quantitation (iTRAQ) were used to profile potential exosomal proteins facilitating nerve regeneration. We observed that locally applied ADSC-exo and ADSC-F-exo significantly enhanced nerve regeneration after nerve crush injury. Pretreatment of ADSCs with FK506 failed to produce exosomes possessing more potent molecules for enhanced nerve regeneration. Proteomic analysis revealed that of 192 exosomal proteins detected in both ADSC-exo and ADSC-F-exo, histone deacetylases (HDACs), amyloid-beta A4 protein (APP), and integrin beta-1 (ITGB1) might be involved in enhancing nerve regeneration.

scholarly journals Exosomes Secreted by Adipose-Derived Stem Cells Following FK506 Stimulation Reduce Autophagy of Macrophages in Spine after Nerve Crush Injury

2021 ◽  
Vol 22 (17) ◽  
pp. 9628
Author(s):  
Pao-Jen Kuo ◽  
Cheng-Shyuan Rau ◽  
Shao-Chun Wu ◽  
Chia-Wei Lin ◽  
Lien-Hung Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Inflammatory Responses ◽  
Immunosuppressive Drug ◽  
Crush Injury ◽  
Sciatic Nerve Crush ◽  
Adipose Derived Stem Cells ◽  
Absolute Quantitation ◽  
Nerve Crush ◽  
Spinal Segments ◽  
Nerve Crush Injury

Macrophages emerge in the milieu around innervated neurons after nerve injuries. Following nerve injury, autophagy is induced in macrophages and affects the regulation of inflammatory responses. It is closely linked to neuroinflammation, while the immunosuppressive drug tacrolimus (FK506) enhances nerve regeneration following nerve crush injury and nerve allotransplantation with additional neuroprotective and neurotrophic functions. The combined use of FK506 and adipose-derived stem cells (ADSCs) was employed in cell therapy for organ transplantation and vascularized composite allotransplantation. This study aimed to investigate the topical application of exosomes secreted by ADSCs following FK506 treatment (ADSC-F-exo) to the injured nerve in a mouse model of sciatic nerve crush injury. Furthermore, isobaric tags for relative and absolute quantitation (iTRAQ) were used to profile the potential exosomal proteins involved in autophagy. Immunohistochemical analysis revealed that nerve crush injuries significantly induced autophagy in the dorsal root ganglia and dorsal horn of the spinal segments. Locally applied ADSC-F-exo significantly reduced autophagy of macrophages in the spinal segments after nerve crush injury. Proteomic analysis showed that of the 22 abundant exosomal proteins detected in ADSC-F-exo, heat shock protein family A member 8 (HSPA8) and eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) are involved in exosome-mediated autophagy reduction.

THE EFFECT OF ADIPOSE TISSUE-DERIVED STEM CELLS IN THE PRESERVATION OF ERECTILE FUNCTION AFTER CAVERNOUS NERVE CRUSH INJURY IN AGED RATS

2009 ◽  
Vol 181 (4S) ◽  
pp. 44-44
Author(s):  
Thomas M Fandel ◽  
Anthony J Bella ◽  
Alan W Shindel ◽  
Guiting Lin ◽  
Maurice M Garcia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Erectile Function ◽  
Crush Injury ◽  
Aged Rats ◽  
Nerve Crush ◽  
Cavernous Nerve ◽  
Nerve Crush Injury

Muscle-derived GDNF facilitates motor nerve recovery following sciatic nerve crush injury

2007 ◽  
Vol 205 (3) ◽  
pp. S92
Author(s):  
Terence M. Myckatyn ◽  
Christina Kenney ◽  
Alice Tong ◽  
Jessica Duan ◽  
Daniel Hunter ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Motor Nerve ◽  
Crush Injury ◽  
Sciatic Nerve Crush ◽  
Nerve Crush ◽  
Nerve Recovery ◽  
Nerve Crush Injury
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