PAX3+skeletal muscle satellite cells retain long-term self-renewal and proliferation

2016 ◽  
Vol 54 (5) ◽  
pp. 943-951 ◽  
Author(s):  
Qiumei Yang ◽  
Jie Yu ◽  
Bing Yu ◽  
Zhiqing Huang ◽  
Keying Zhang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Self Renewal ◽  
Muscle Satellite Cells ◽  
Skeletal Muscle Satellite Cells

scholarly journals  -catenin promotes self-renewal of skeletal-muscle satellite cells

2008 ◽  
Vol 121 (9) ◽  
pp. 1373-1382 ◽  
Author(s):  
A. Perez-Ruiz ◽  
Y. Ono ◽  
V. F. Gnocchi ◽  
P. S. Zammit
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Self Renewal ◽  
Muscle Satellite Cells ◽  
Skeletal Muscle Satellite Cells

scholarly journals  -catenin promotes self-renewal of skeletal-muscle satellite cells

2011 ◽  
Vol 124 (23) ◽  
pp. 4125-4125 ◽  
Author(s):  
A. Perez-Ruiz ◽  
Y. Ono ◽  
V. F. Gnocchi ◽  
P. S. Zammit
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Self Renewal ◽  
Muscle Satellite Cells ◽  
Skeletal Muscle Satellite Cells

scholarly journals miR-21-5p Regulates the Proliferation and Differentiation of Skeletal Muscle Satellite Cells by Targeting KLF3 in Chicken

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 814
Author(s):  
Donghao Zhang ◽  
Jinshan Ran ◽  
Jingjing Li ◽  
Chunlin Yu ◽  
Zhifu Cui ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Muscle Development ◽  
Target Genes ◽  
Cell Number ◽  
Sequencing Data ◽  
Proliferation Markers ◽  
Muscle Satellite Cells ◽  
Skeletal Muscle Satellite Cells ◽  
Proliferation And Differentiation

The proliferation and differentiation of skeletal muscle satellite cells (SMSCs) play an important role in the development of skeletal muscle. Our previous sequencing data showed that miR-21-5p is one of the most abundant miRNAs in chicken skeletal muscle. Therefore, in this study, the spatiotemporal expression of miR-21-5p and its effects on skeletal muscle development of chickens were explored using in vitro cultured SMSCs as a model. The results in this study showed that miR-21-5p was highly expressed in the skeletal muscle of chickens. The overexpression of miR-21-5p promoted the proliferation of SMSCs as evidenced by increased cell viability, increased cell number in the proliferative phase, and increased mRNA and protein expression of proliferation markers including PCNA, CDK2, and CCND1. Moreover, it was revealed that miR-21-5p promotes the formation of myotubes by modulating the expression of myogenic markers including MyoG, MyoD, and MyHC, whereas knockdown of miR-21-5p showed the opposite result. Gene prediction and dual fluorescence analysis confirmed that KLF3 was one of the direct target genes of miR-21-5p. We confirmed that, contrary to the function of miR-21-5p, KLF3 plays a negative role in the proliferation and differentiation of SMSCs. Si-KLF3 promotes cell number and proliferation activity, as well as the cell differentiation processes. Our results demonstrated that miR-21-5p promotes the proliferation and differentiation of SMSCs by targeting KLF3. Collectively, the results obtained in this study laid a foundation for exploring the mechanism through which miR-21-5p regulates SMSCs.

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