Growth-associated protein-43 expression in cocultures of dorsal root ganglion neurons and skeletal muscle cells with different neurotrophins

2013 ◽  
Vol 47 (6) ◽  
pp. 909-915 ◽  
Author(s):  
Weiwei Zhang ◽  
Yajun Miao ◽  
Ziying Xing ◽  
Hao Li ◽  
Huangxiang Liu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Muscle Cells ◽  
Dorsal Root Ganglion Neurons ◽  
Skeletal Muscle Cells ◽  
Ganglion Neurons

scholarly journals Dorsal Root Ganglion Neurons Innervating Skeletal Muscle Respond to Physiological Combinations of Protons, ATP, and Lactate Mediated by ASIC, P2X, and TRPV1

2008 ◽  
Vol 100 (3) ◽  
pp. 1184-1201 ◽  
Author(s):  
Alan R. Light ◽  
Ronald W. Hughen ◽  
Jie Zhang ◽  
Jon Rainier ◽  
Zhuqing Liu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Dorsal Root Ganglion ◽  
Calcium Imaging ◽  
Dorsal Root ◽  
Dorsal Root Ganglion Neurons ◽  
Drg Neurons ◽  
Molecular Receptors ◽  
Trpv1 Receptors ◽  
Ganglion Neurons ◽  
Two Populations

The adequate stimuli and molecular receptors for muscle metaboreceptors and nociceptors are still under investigation. We used calcium imaging of cultured primary sensory dorsal root ganglion (DRG) neurons from C57Bl/6 mice to determine candidates for metabolites that could be the adequate stimuli and receptors that could detect these stimuli. Retrograde DiI labeling determined that some of these neurons innervated skeletal muscle. We found that combinations of protons, ATP, and lactate were much more effective than individually applied compounds for activating rapid calcium increases in muscle-innervating dorsal root ganglion neurons. Antagonists for P2X, ASIC, and TRPV1 receptors suggested that these three receptors act together to detect protons, ATP, and lactate when presented together in physiologically relevant concentrations. Two populations of muscle-innervating DRG neurons were found. One responded to low metabolite levels (likely nonnoxious) and used ASIC3, P2X5, and TRPV1 as molecular receptors to detect these metabolites. The other responded to high levels of metabolites (likely noxious) and used ASIC3, P2X4, and TRPV1 as their molecular receptors. We conclude that a combination of ASIC, P2X5 and/or P2X4, and TRPV1 are the molecular receptors used to detect metabolites by muscle-innervating sensory neurons. We further conclude that the adequate stimuli for muscle metaboreceptors and nociceptors are combinations of protons, ATP, and lactate.

Co-administration of Monosialoganglioside and Skeletal Muscle Cells on Dorsal Root Ganglion Neuronal Phenotypes In Vitro

2009 ◽  
Vol 30 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Lihong Wang ◽  
Hao Li ◽  
Zhen Liu ◽  
Xiaobo Xu ◽  
Huaijing Wang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Muscle Cells ◽  
Skeletal Muscle Cells
Sign in / Sign up

Export Citation Format

Share Document