Effects of hyperbaric exposure with high oxygen concentration on glucose and insulin levels and skeletal muscle-fiber properties in diabetic rats

2007 ◽  
Vol 35 (3) ◽  
pp. 337-343 ◽  
Author(s):  
Koichiro Yasuda ◽  
Tetsuya Adachi ◽  
Ning Gu ◽  
Akiko Matsumoto ◽  
Tetsuro Matsunaga ◽  
...  
Author(s):  
Ryoichi Mitsuzono ◽  
Shigeru Katsuta ◽  
Yoji Kanao ◽  
Hirofumi Miyata ◽  
Kazunori Asaba ◽  
...  

2005 ◽  
Vol 52 (2) ◽  
pp. 146-152 ◽  
Author(s):  
Akihiko Ishihara ◽  
Fuminori Kawano ◽  
Tomonori Okiura ◽  
Fumiki Morimatsu ◽  
Yoshinobu Ohira

2006 ◽  
Vol 29 (6) ◽  
pp. 452-459 ◽  
Author(s):  
A. Matsumoto ◽  
T. Okiura ◽  
F. Morimatsu ◽  
Y. Ohira ◽  
A. Ishihara

Author(s):  
Joachim R. Sommer ◽  
Teresa High ◽  
Betty Scherer ◽  
Isaiah Taylor ◽  
Rashid Nassar

We have developed a model that allows the quick-freezing at known time intervals following electrical field stimulation of a single, intact frog skeletal muscle fiber isolated by sharp dissection. The preparation is used for studying high resolution morphology by freeze-substitution and freeze-fracture and for electron probe x-ray microanlysis of sudden calcium displacement from intracellular stores in freeze-dried cryosections, all in the same fiber. We now show the feasibility and instrumentation of new methodology for stimulating a single, intact skeletal muscle fiber at a point resulting in the propagation of an action potential, followed by quick-freezing with sub-millisecond temporal resolution after electrical stimulation, followed by multiple sampling of the frozen muscle fiber for freeze-substitution, freeze-fracture (not shown) and cryosectionmg. This model, at once serving as its own control and obviating consideration of variances between different fibers, frogs etc., is useful to investigate structural and topochemical alterations occurring in the wake of an action potential.


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