scholarly journals Alternative polyadenylation diversifies post‐transcriptional regulation by selective RNA –protein interactions

2014 ◽  
Vol 10 (2) ◽  
pp. 719 ◽  
Author(s):  
Ishaan Gupta ◽  
Sandra Clauder‐Münster ◽  
Bernd Klaus ◽  
Aino I Järvelin ◽  
Raeka S Aiyar ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Protein Interactions ◽  
Alternative Polyadenylation ◽  
Post Transcriptional Regulation

Post-transcriptional regulation of gene expression by Unr

2015 ◽  
Vol 43 (3) ◽  
pp. 323-327 ◽  
Author(s):  
Swagat Ray ◽  
Pól Ó Catnaigh ◽  
Emma C. Anderson
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Protein Interactions ◽  
Rna Binding ◽  
Regulation Of Gene Expression ◽  
Negative Regulator ◽  
Cellular Translation ◽  
Cellular Mrnas ◽  
Internal Initiation ◽  
Post Transcriptional Regulation

Unr (upstream of N-ras) is a eukaryotic RNA-binding protein that has a number of roles in the post-transcriptional regulation of gene expression. Originally identified as an activator of internal initiation of picornavirus translation, it has since been shown to act as an activator and inhibitor of cellular translation and as a positive and negative regulator of mRNA stability, regulating cellular processes such as mitosis and apoptosis. The different post-transcriptional functions of Unr depend on the identity of its mRNA and protein partners and can vary with cell type and changing cellular conditions. Recent high-throughput analyses of RNA–protein interactions indicate that Unr binds to a large subset of cellular mRNAs, suggesting that Unr may play a wider role in translational responses to cellular signals than previously thought.

High-throughput sequencing methods to study neuronal RNA–protein interactions

2009 ◽  
Vol 37 (6) ◽  
pp. 1278-1280 ◽  
Author(s):  
Jernej Ule
Keyword(s):  
Transcriptional Regulation ◽  
High Throughput ◽  
Protein Interactions ◽  
Translational Control ◽  
High Throughput Sequencing ◽  
Ribosome Profiling ◽  
Cross Linking ◽  
Rnase Protection ◽  
Temporal Precision ◽  
Post Transcriptional Regulation

UV-cross-linking and RNase protection, combined with high-throughput sequencing, have provided global maps of RNA sites bound by individual proteins or ribosomes. Using a stringent purification protocol, UV-CLIP (UV-cross-linking and immunoprecipitation) was able to identify intronic and exonic sites bound by splicing regulators in mouse brain tissue. Ribosome profiling has been used to quantify ribosome density on budding yeast mRNAs under different environmental conditions. Post-transcriptional regulation in neurons requires high spatial and temporal precision, as is evident from the role of localized translational control in synaptic plasticity. It remains to be seen if the high-throughput methods can be applied quantitatively to study the dynamics of RNP (ribonucleoprotein) remodelling in specific neuronal populations during the neurodegenerative process. It is certain, however, that applications of new biochemical techniques followed by high-throughput sequencing will continue to provide important insights into the mechanisms of neuronal post-transcriptional regulation.

scholarly journals Coordinated Post-transcriptional Regulation of Hsp70.3 Gene Expression by MicroRNA and Alternative Polyadenylation

2011 ◽  
Vol 286 (34) ◽  
pp. 29828-29837 ◽  
Author(s):  
Michael Tranter ◽  
Robert N. Helsley ◽  
Waltke R. Paulding ◽  
Michael McGuinness ◽  
Cole Brokamp ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Alternative Polyadenylation ◽  
Post Transcriptional Regulation

Pka, Ras and RGS Protein Interactions Regulate Activity of AflR, a Zn(II)2Cys6 Transcription Factor in Aspergillus nidulans

Genetics ◽  
2003 ◽  
Vol 165 (3) ◽  
pp. 1095-1104
Author(s):  
Kiminori Shimizu ◽  
Julie K Hicks ◽  
Tzu-Pi Huang ◽  
Nancy P Keller
Keyword(s):  
Transcription Factor ◽  
Transcriptional Regulation ◽  
Aspergillus Nidulans ◽  
G Protein ◽  
Protein Interactions ◽  
Transcriptional Control ◽  
G Protein Signaling ◽  
Protein Signaling ◽  
Rgs Protein ◽  
Post Transcriptional Regulation

Abstract Sterigmatocystin (ST) is a carcinogenic polyketide produced by several filamentous fungi including Aspergillus nidulans. Expression of ST biosynthetic genes (stc genes) requires activity of a Zn(II)2Cys6 transcription factor, AflR. aflR is transcriptionally and post-transcriptionally regulated by a G-protein/cAMP/protein kinase A (PkaA) signaling pathway involving FlbA, an RGS (regulator of G-protein signaling) protein. Prior genetic data showed that FlbA transcriptional regulation of aflR was PkaA dependent. Here we show that mutation of three PkaA phosphorylation sites in AflR allows resumption of stc expression in an overexpression pkaA background but does not remediate stc expression in a ΔflbA background. This demonstrates negative regulation of AflR activity by phosphorylation and shows that FlbA post-transcriptional regulation of aflR is PkaA independent. AflR nucleocytoplasmic location further supports PkaA-independent regulation of AflR by FlbA. GFP-tagged AflR is localized to the cytoplasm when pkaA is overexpressed but nuclearly located in a ΔflbA background. aflR is also transcriptionally and post-transcriptionally regulated by RasA. RasA transcriptional control of aflR is PkaA independent but RasA post-transcriptional control of AflR is partially mediated by PkaA.

scholarly journals Post‐transcriptional regulation of Hsp70.3 expression by miRNAs and alternative polyadenylation

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Michael Tranter ◽  
Michael McGuinness ◽  
Xiaoping Ren ◽  
Robert N. Helsley ◽  
Waltke R. Paulding ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Alternative Polyadenylation ◽  
Post Transcriptional Regulation

43-OR: Post-transcriptional Regulation of Slc16a1 mRNA Is Essential for Maintaining Normal ß-Cell Function

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 43-OR
Author(s):  
DINA MOSTAFA ◽  
AKINORI TAKAHASHI ◽  
TADASHI YAMAMOTO
Keyword(s):  
Transcriptional Regulation ◽  
Cell Function ◽  
Post Transcriptional Regulation
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