scholarly journals Rapid in vivo detection of rat spinal cord injury with double-diffusion-encoded magnetic resonance spectroscopy

2016 ◽  
Vol 77 (4) ◽  
pp. 1639-1649 ◽  
Author(s):  
Nathan P. Skinner ◽  
Shekar N. Kurpad ◽  
Brian D. Schmit ◽  
L. Tugan Muftuler ◽  
Matthew D. Budde
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Double Diffusion ◽  
Resonance Spectroscopy ◽  
Rat Spinal Cord ◽  
In Vivo Detection ◽  
Cord Injury

Phosphorus-31 Magnetic Resonance Spectroscopy Studies of Pig Spinal Cord Injury

1997 ◽  
Vol 32 (7) ◽  
pp. 382-388 ◽  
Author(s):  
MINORU AKINO ◽  
J. MICHAEL O'DONNELL ◽  
PIERRE-MARIE L. ROBITAILLE ◽  
BRADFORD T. STOKES
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Resonance Spectroscopy ◽  
Cord Injury ◽  
Spectroscopy Studies

scholarly journals Longitudinal In Vivo Diffusion Magnetic Resonance Imaging Remote from the Lesion Site in Rat Spinal Cord Injury

2019 ◽  
Vol 36 (9) ◽  
pp. 1389-1398 ◽  
Author(s):  
Alice Motovylyak ◽  
Nathan P. Skinner ◽  
Brian D. Schmit ◽  
Natasha Wilkins ◽  
Shekar N. Kurpad ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Magnetic Resonance ◽  
Diffusion Magnetic Resonance Imaging ◽  
Lesion Site ◽  
Resonance Imaging ◽  
Rat Spinal Cord ◽  
Cord Injury

scholarly journals Imaging of Inflammation in Spinal Cord Injury: Novel Insights on the Usage of PFC-Based Contrast Agents

Biomedicines ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 379
Author(s):  
Francesca Garello ◽  
Marina Boido ◽  
Martina Miglietti ◽  
Valeria Bitonto ◽  
Marco Zenzola ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Magnetic Resonance ◽  
Half Life ◽  
Cell Tracking ◽  
Resonance Spectroscopy ◽  
Cord Injury ◽  
The Impact

Labeling of macrophages with perfluorocarbon(PFC)-based compounds allows the visualization of inflammatory processes by 19F-magnetic resonance imaging (19F-MRI), due to the absence of endogenous background. Even if PFC-labeling of monocytes/macrophages has been largely investigated and used, information is lacking about the impact of these agents over the polarization towards one of their cell subsets and on the best way to image them. In the present work, a PFC-based nanoemulsion was developed to monitor the course of inflammation in a model of spinal cord injury (SCI), a pathology in which the understanding of immunological events is of utmost importance to select the optimal therapeutic strategies. The effects of PFC over macrophage polarization were studied in vitro, on cultured macrophages, and in vivo, in a mouse SCI model, by testing and comparing various cell tracking protocols, including single and multiple administrations, the use of MRI or Point Resolved Spectroscopy (PRESS), and application of pre-saturation of Kupffer cells. The blood half-life of nanoemulsion was also investigated by 19F Magnetic Resonance Spectroscopy (MRS). In vitro and in vivo results indicate the occurrence of a switch towards the M2 (anti-inflammatory) phenotype, suggesting a possible theranostic function of these nanoparticles. The comparative work presented here allows the reader to select the most appropriate protocol according to the research objectives (quantitative data acquisition, visual monitoring of macrophage recruitment, theranostic purpose, rapid MRI acquisition, etc.). Finally, the method developed here to determine the blood half-life of the PFC nanoemulsion can be extended to other fluorinated compounds.

scholarly journals Metabolites of neuroinflammation relate to neuropathic pain after spinal cord injury

Neurology ◽  
2020 ◽  
Vol 95 (7) ◽  
pp. e805-e814
Author(s):  
Dario Pfyffer ◽  
Patrik O. Wyss ◽  
Eveline Huber ◽  
Armin Curt ◽  
Anke Henning ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Neuropathic Pain ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Cervical Cord ◽  
Resonance Spectroscopy ◽  
Pain Sensation ◽  
Total N ◽  
Cord Injury

ObjectiveTo determine whether cervical cord levels of metabolites are associated with pain sensation after spinal cord injury (SCI) by performing magnetic resonance spectroscopy in patients with SCI with and without neuropathic pain (NP).MethodsCervical cord single-voxel spectroscopic data of 24 patients with SCI (14 with NP, 10 pain-free) and 21 healthy controls were acquired at C2/3 to investigate metabolite ratios associated with neuroinflammation (choline-containing compounds to myoinositol [tCho/mI]) and neurodegeneration (total N-acetylaspartate to myo-inositol [tNAA/mI]). NP levels were measured, and Spearman correlation tests assessed associations between metabolite levels, cord atrophy, and pinprick score.ResultsIn patients with NP, tCho/mI levels were increased (p = 0.024) compared to pain-free patients and negatively related to cord atrophy (p = 0.006, r = 0.714). Better pinprick score was associated with higher tCho/mI levels (p = 0.032, r = 0.574). In pain-free patients, tCho/mI levels were not related to cord atrophy (p = 0.881, r = 0.055) or pinprick score (p = 0.676, r = 0.152). tNAA/mI levels were similar in both patient groups (p = 0.396) and were not associated with pinprick score in patients with NP (p = 0.405, r = 0.242) and pain-free patients (p = 0.117, r = 0.527).ConclusionsNeuroinflammatory metabolite levels (i.e., tCho/mI) were elevated in patients with NP, its magnitude being associated with less cord atrophy and greater pain sensation (e.g., pinprick score). This suggests that patients with NP have more residual spinal tissue and greater metabolite turnover than pain-free patients. Neurodegenerative metabolite levels (i.e., tNAA/mI) were associated with greater cord atrophy but unrelated to NP. Identifying the metabolic NP signature provides new NP treatment targets and could improve patient stratification in interventional trials.Classification of evidenceThis study provides Class II evidence that levels of magnetic resonance spectroscopy–identified metabolites of neuroinflammation were elevated in patients with SCI with NP compared to those without NP.

scholarly journals In vivo detection of citrate in brain tumors by1H magnetic resonance spectroscopy at 3T

2013 ◽  
Vol 72 (2) ◽  
pp. 316-323 ◽  
Author(s):  
Changho Choi ◽  
Sandeep K. Ganji ◽  
Akshay Madan ◽  
Keith M. Hulsey ◽  
Zhongxu An ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Brain Tumors ◽  
Resonance Spectroscopy ◽  
In Vivo Detection
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