scholarly journals Microscopic diffusivity compartmentation in formalin-fixed prostate tissue

2012 ◽  
Vol 68 (2) ◽  
pp. spcone-spcone
Author(s):  
Roger M. Bourne ◽  
Nyoman Kurniawan ◽  
Gary Cowin ◽  
Timothy Stait-Gardner ◽  
Paul Sved ◽  
...  
Keyword(s):  
Prostate Tissue ◽  
Formalin Fixed

scholarly journals Biexponential diffusion decay in formalin-fixed prostate tissue: Preliminary findings

2011 ◽  
Vol 68 (3) ◽  
pp. 954-959 ◽  
Author(s):  
Roger M. Bourne ◽  
Nyoman Kurniawan ◽  
Gary Cowin ◽  
Timothy Stait-Gardner ◽  
Paul Sved ◽  
...  
Keyword(s):  
Prostate Tissue ◽  
Diffusion Decay ◽  
Formalin Fixed

scholarly journals Microscopic diffusion anisotropy in formalin fixed prostate tissue: Preliminary findings

2012 ◽  
Vol 68 (6) ◽  
pp. 1943-1948 ◽  
Author(s):  
Roger M. Bourne ◽  
Nyoman Kurniawan ◽  
Gary Cowin ◽  
Paul Sved ◽  
Geoffrey Watson
Keyword(s):  
Prostate Tissue ◽  
Diffusion Anisotropy ◽  
Formalin Fixed

P17: Array-based comparative genomic hybridization of laser-microdissected epithelia from formalin fixed and paraffin embedded breast and prostate tissue

2005 ◽  
Vol 48 (4) ◽  
pp. 462-463
Author(s):  
Ulrike Montag ◽  
Uta Strassburger ◽  
Sabine Steinhoff ◽  
Katrin Friedrich ◽  
Esmeralda Castanos-Velez ◽  
...  
Keyword(s):  
Comparative Genomic Hybridization ◽  
Prostate Tissue ◽  
Comparative Genomic ◽  
Genomic Hybridization ◽  
Formalin Fixed

scholarly journals Challenges in Cancer Biomarker Discovery Exemplified by the Identification of Diagnostic MicroRNAs in Prostate Tissues

2020 ◽  
Vol 2020 ◽  
pp. 1-4
Author(s):  
Filip Ambrozkiewicz ◽  
Jakub Karczmarski ◽  
Maria Kulecka ◽  
Agnieszka Paziewska ◽  
Magdalena Cybulska ◽  
...  
Keyword(s):  
Biomarker Discovery ◽  
Prostate Tissue ◽  
Operating Characteristics ◽  
Prostate Hyperplasia ◽  
Tissue Sections ◽  
Formalin Fixed Paraffin ◽  
Receiver Operating Characteristics Curve ◽  
Formalin Fixed Paraffin Embedded ◽  
Benign Prostate ◽  
Formalin Fixed

Identification and clinical translation of routinely tested biomarkers require a complex and multistep workflow. Here, we described a confirmatory process estimating the utility of previously identified candidate tissue miRNAs for diagnosis of prostate cancer (PCa). RNA was isolated from formalin-fixed paraffin-embedded (FFPE) prostate tissue surgically resected from 44 patients with PCa and 24 patients with benign prostate hyperplasia (BPH). Of the 92 RNA samples obtained, 68 represented 42 malignant (PCa) areas and 26 represented nonmalignant (PCa 0%) areas of the prostate tissue sections. The levels of miR-32-5p, miR-183-5p, miR-141-5p, miR-187-3p, miR-375, miR-663b, miR-615-3p, miR-205-5p, miR-221-3p, and miR-222-3p were evaluated using Exiqon chemistry. Five (miR-32-5p, miR-141-5p, miR-187-3p, miR-375, and miR-615-3p), one (miR-32-5p), and two (miR-32-5p and miR-141-5p) miRNAs discriminated between BPH and areas of cancer-bearing prostate tissue harboring different numbers of cancer cells (PCa 15–70%, PCa 2–10%, and PCA 0%, respectively), with an area under the receiver operating characteristics curve (AUC-ROC) > 0.9. Only miRNA 32-5p discriminated BPH specimens from sections of cancer-bearing prostate tissue with a low percentage, a high percentage, or no dysplastic cells. miR-32-5p could be considered as potential diagnostic biomarker discriminating BPH from noncancerous areas within cancer-bearing prostate tissue. However, further clinical studies are warranted to confirm its diagnostic utility.

scholarly journals Microscopic diffusivity compartmentation in formalin-fixed prostate tissue

2011 ◽  
Vol 68 (2) ◽  
pp. 614-620 ◽  
Author(s):  
Roger M. Bourne ◽  
Nyoman Kurniawan ◽  
Gary Cowin ◽  
Timothy Stait-Gardner ◽  
Paul Sved ◽  
...  
Keyword(s):  
Prostate Tissue ◽  
Formalin Fixed

Ultrastructural Localization of Acid Mucosubstance in Skeletal Muscle

1967 ◽  
Vol 25 ◽  
pp. 52-53 ◽  
Author(s):  
William J. Dougherty ◽  
Samuel S. Spicer
Keyword(s):  
Striated Muscle ◽  
Divalent Cations ◽  
Ultrastructural Localization ◽  
Major Elements ◽  
Frozen Sections ◽  
Thorium Dioxide ◽  
Iron Solution ◽  
Coupling System ◽  
Psoas Muscles ◽  
Formalin Fixed

In recent years, considerable attention has focused on the morphological nature of the excitation-contraction coupling system of striated muscle. Since the study of Porter and Palade, it has become evident that the sarcoplastic reticulum (SR) and transverse tubules constitute the major elements of this system. The problem still exists, however, of determining the mechamisms by which the signal to interdigitate is presented to the thick and thin myofilaments. This problem appears to center on the movement of Ca++ions between myofilaments and SR. Recently, Philpott and Goldstein reported acid mucosubstance associated with the SR of fish branchial muscle using the colloidal thorium dioxide technique, and suggested that this material may serve to bind or release divalent cations such as Ca++. In the present study, Hale's iron solution adapted to electron microscopy was applied to formalin-fixed myofibrils isolated from glycerol-extracted rabbit psoas muscles and to frozen sections of formalin-fixed rat psoas muscles.

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