scholarly journals High-resolution sodium imaging of human brain at 7 T

2011 ◽  
Vol 68 (1) ◽  
pp. 227-233 ◽  
Author(s):  
Yongxian Qian ◽  
Tiejun Zhao ◽  
Hai Zheng ◽  
Jonathan Weimer ◽  
Fernando E. Boada
Keyword(s):  
High Resolution ◽  
Human Brain ◽  
Sodium Imaging

scholarly journals Application of Airy beam light sheet microscopy to examine early neurodevelopmental structures in 3D hiPSC-derived human cortical spheroids

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Dwaipayan Adhya ◽  
George Chennell ◽  
James A. Crowe ◽  
Eva P. Valencia-Alarcón ◽  
James Seyforth ◽  
...  
Keyword(s):  
High Resolution ◽  
Human Brain ◽  
Light Sheet ◽  
Autism Spectrum ◽  
Model Systems ◽  
Large Field ◽  
Autism Spectrum Conditions ◽  
Patient Specific ◽  
Airy Beam

Abstract Background The inability to observe relevant biological processes in vivo significantly restricts human neurodevelopmental research. Advances in appropriate in vitro model systems, including patient-specific human brain organoids and human cortical spheroids (hCSs), offer a pragmatic solution to this issue. In particular, hCSs are an accessible method for generating homogenous organoids of dorsal telencephalic fate, which recapitulate key aspects of human corticogenesis, including the formation of neural rosettes—in vitro correlates of the neural tube. These neurogenic niches give rise to neural progenitors that subsequently differentiate into neurons. Studies differentiating induced pluripotent stem cells (hiPSCs) in 2D have linked atypical formation of neural rosettes with neurodevelopmental disorders such as autism spectrum conditions. Thus far, however, conventional methods of tissue preparation in this field limit the ability to image these structures in three-dimensions within intact hCS or other 3D preparations. To overcome this limitation, we have sought to optimise a methodological approach to process hCSs to maximise the utility of a novel Airy-beam light sheet microscope (ALSM) to acquire high resolution volumetric images of internal structures within hCS representative of early developmental time points. Results Conventional approaches to imaging hCS by confocal microscopy were limited in their ability to image effectively into intact spheroids. Conversely, volumetric acquisition by ALSM offered superior imaging through intact, non-clarified, in vitro tissues, in both speed and resolution when compared to conventional confocal imaging systems. Furthermore, optimised immunohistochemistry and optical clearing of hCSs afforded improved imaging at depth. This permitted visualization of the morphology of the inner lumen of neural rosettes. Conclusion We present an optimized methodology that takes advantage of an ALSM system that can rapidly image intact 3D brain organoids at high resolution while retaining a large field of view. This imaging modality can be applied to both non-cleared and cleared in vitro human brain spheroids derived from hiPSCs for precise examination of their internal 3D structures. This process represents a rapid, highly efficient method to examine and quantify in 3D the formation of key structures required for the coordination of neurodevelopmental processes in both health and disease states. We posit that this approach would facilitate investigation of human neurodevelopmental processes in vitro.

High resolution metabolomic analysis of ASD human brain uncovers novel biomarkers of disease

Metabolomics ◽  
2016 ◽  
Vol 12 (4) ◽  
Author(s):  
Stewart F. Graham ◽  
Olivier P. Chevallier ◽  
Praveen Kumar ◽  
Onur Türkoğlu ◽  
Ray O. Bahado-Singh
Keyword(s):  
High Resolution ◽  
Human Brain ◽  
Metabolomic Analysis ◽  
Novel Biomarkers

scholarly journals High-Resolution Mapping of Myeloarchitecture In Vivo: Localization of Auditory Areas in the Human Brain

2014 ◽  
Vol 25 (10) ◽  
pp. 3394-3405 ◽  
Author(s):  
Federico De Martino ◽  
Michelle Moerel ◽  
Junqian Xu ◽  
Pierre-Francois van de Moortele ◽  
Kamil Ugurbil ◽  
...  
Keyword(s):  
High Resolution ◽  
Human Brain ◽  
High Resolution Mapping ◽  
Resolution Mapping

scholarly journals Perfusion-based high-resolution functional imaging in the human brain at 7 Tesla

2002 ◽  
Vol 47 (5) ◽  
pp. 903-911 ◽  
Author(s):  
Josef Pfeuffer ◽  
Gregor Adriany ◽  
Amir Shmuel ◽  
Essa Yacoub ◽  
Pierre-Francois Van De Moortele ◽  
...  
Keyword(s):  
High Resolution ◽  
Human Brain ◽  
Functional Imaging ◽  
7 Tesla

High-resolution mechanical imaging of the human brain by three-dimensional multifrequency magnetic resonance elastography at 7T

NeuroImage ◽  
2014 ◽  
Vol 90 ◽  
pp. 308-314 ◽  
Author(s):  
Jürgen Braun ◽  
Jing Guo ◽  
Ralf Lützkendorf ◽  
Jörg Stadler ◽  
Sebastian Papazoglou ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
High Resolution ◽  
Human Brain ◽  
Three Dimensional ◽  
Magnetic Resonance Elastography ◽  
Mechanical Imaging

Frozen tissue preparation for high-resolution multiplex histological analyses of human brain specimens

2017 ◽  
Vol 135 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Fangjie Shao ◽  
Wenhong Jiang ◽  
Qingqing Gao ◽  
Baizhou Li ◽  
Chongran Sun ◽  
...  
Keyword(s):  
High Resolution ◽  
Human Brain ◽  
Tissue Preparation ◽  
Frozen Tissue

Phased array detectors and an automated intensity-correction algorithm for high-resolution MR imaging of the human brain

1995 ◽  
Vol 34 (3) ◽  
pp. 433-439 ◽  
Author(s):  
Lawrence L. Wald ◽  
Lucas Carvajal ◽  
Susan E. Moyher ◽  
Sarah J. Nelson ◽  
P. Ellen Grant ◽  
...  
Keyword(s):  
High Resolution ◽  
Mr Imaging ◽  
Human Brain ◽  
Phased Array ◽  
Correction Algorithm ◽  
Array Detectors
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