scholarly journals Increased anatomical detail by in vitro MR microscopy with a modified Golgi impregnation method

2010 ◽  
Vol 63 (5) ◽  
pp. 1391-1397 ◽  
Author(s):  
Xiaowei Zhang ◽  
Elaine L. Bearer ◽  
Adriana T. Perles-Barbacaru ◽  
Russell E. Jacobs
Keyword(s):  
Impregnation Method ◽  
Mr Microscopy ◽  
Golgi Impregnation ◽  
Anatomical Detail

Tri-modal imaging of gold-dotted magnetic nanoparticles for magnetic resonance imaging, computed tomography and intravascular ultrasound: an in vitro study

Nanomedicine ◽  
2020 ◽  
Vol 15 (25) ◽  
pp. 2433-2445
Author(s):  
Joel Kuhn ◽  
Giorgos Papanastasiou ◽  
Cheuk-Wai Tai ◽  
Carmel M Moran ◽  
Maurits A Jansen ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Magnetic Nanoparticles ◽  
Magnetic Resonance ◽  
Intravascular Ultrasound ◽  
In Vitro Study ◽  
In Vitro Cytotoxicity ◽  
Impregnation Method ◽  
Transmission Electron ◽  
Ivus Imaging

Aim: To examine the multimodal contrasting ability of gold-dotted magnetic nanoparticles (Au*MNPs) for magnetic resonance (MR), computed tomography (CT) and intravascular ultrasound (IVUS) imaging. Materials & methods: Au*MNPs were prepared by adapting an impregnation method, without using surface capping reagents and characterized (transmission electron microscopy, x-ray diffraction and Fourier-transform infrared spectroscopy) with their in vitro cytotoxicity assessed, followed by imaging assessments. Results: The contrast-enhancing ability of Au*MNPs was shown to be concentration-dependent across MR, CT and IVUS imaging. The Au content of the Au*MNP led to evident increases of the IVUS signal. Conclusion: We demonstrated that Au*MNPs showed concentration-dependent contrast-enhancing ability in MRI and CT imaging, and for the first-time in IVUS imaging due to the Au content. These Au*MNPs are promising toward solidifying tri-modal imaging-based theragnostics.

Research on the Preparation of HA/PEEK Gradient Composites by Impregnation Method and the Biosecurity

2017 ◽  
Vol 893 ◽  
pp. 35-42 ◽  
Author(s):  
Rui Ma ◽  
Ling Jun Dai ◽  
Lin Wang ◽  
Yu Dian Song
Keyword(s):  
Mechanical Properties ◽  
Bone Formation ◽  
Structural Design ◽  
Injection Moulding ◽  
In Vitro Cytotoxicity ◽  
Leach Liquor ◽  
Cytotoxicity Test ◽  
Impregnation Method ◽  
The Poor

This study prepares the HA/PEEK gradient composites with special structural design by impregnation method, which eases the contradiction of the HA/PEEK composites between the lack of bioactivity due to low HA contents and the poor mechanical properties due to high HA contents, and effectively improves the bioactivity and mechanical properties of the PEEK-based materials, and then evaluates the biosecurity of HA/PEEK gradient composites. The main functional cell of bone formation—osteoblast is adopted for the co-culture with PEEK, HA/PEEK gradient composites and the leach liquor, and the effects of PEEK and HA/PEEK gradient composites on the proliferative functions of osteoblast are studied by in vitro cytotoxicity test. The results show that no harmful admixtures are produced in the process of injection moulding, and the leach liquors of PEEK and HA/PEEK gradient composites have no effects on the proliferative functions of osteoblast, with good osteoblast compatibility.

Ce(IV) Doped TiO2 Nanoparticles and the Effect on Proliferation of BEL7402 Human Hepatoma Cells

2007 ◽  
Vol 330-332 ◽  
pp. 307-310
Author(s):  
L. Wang ◽  
J. Mao ◽  
X.L. Zhang ◽  
M.J. Tu ◽  
B. Kan ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Titanium Dioxide Nanoparticles ◽  
Hepatoma Cells ◽  
Control Group ◽  
Impregnation Method ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Transmission Electron ◽  
Induced Apoptosis

To investigate nanoparticles’ antitumor effect, a globose 30~35nm Ce(IV) doped Titanium dioxide nanoparticles (CDT) were prepared by impregnation method and characterized by X-ray diffraction (XRD) and transmission electron microscopy(TEM). Proliferation of BEL7402 human Hepatoma cells was studied in vitro by using fluorescence microscopy and Flow Cytometry. The results show that cerium elements doping enhanced thermal stability of nano-size titanium dioxide to 800°C. With UV irradiated for 8min, BEL7402 were induced a dose-dependent apoptosis by CDT, about 28.2% , 41.5% and 88.3% cells were induced apoptosis after 24h at concentration of 60, 120 and 180ug/ml respectively, relative to 3.9% of that of control group cell just only in the presence of UV.

A Golgi impregnation technique for thin brain slices maintained in vitro

1980 ◽  
Vol 2 (4) ◽  
pp. 363-371 ◽  
Author(s):  
Kristen M. Harris ◽  
William L.R. Cruce ◽  
William T. Greenough ◽  
Timothy J. Teyler
Keyword(s):  
Brain Slices ◽  
Golgi Impregnation ◽  
Impregnation Technique

scholarly journals Influence of the Impregnation Technique on the Release of Esomeprazole from Various Bioaerogels

Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1882
Author(s):  
Milica Pantić ◽  
Katja Andrina Kravanja ◽  
Željko Knez ◽  
Zoran Novak
Keyword(s):  
Sol Gel ◽  
In Vitro Dissolution ◽  
Impregnation Method ◽  
Impregnation Technique ◽  
Supercritical Impregnation ◽  
Release Studies ◽  
Sol Gel Synthesis ◽  
Adsorption Desorption ◽  
And Diffusion

The presented study shows the possibility of using bioaerogels, namely neat alginate, pectin, chitosan aerogels, and alginate and pectin aerogels coated with chitosan, as drug delivery systems for esomeprazole. Two different techniques were used for the impregnation of esomeprazole: Supercritical impregnation, and diffusion via ethanol during the sol-gel synthesis. The prepared samples were characterized by employing N2 adsorption-desorption analysis, TGA/DSC, and FTIR. The achieved loadings were satisfactory for all the tested samples and showed to be dependent on the technique used for impregnation. In all cases, higher loadings were achieved when impregnation via diffusion from ethanol was used. Extensive release studies were performed for all impregnated samples. The in vitro dissolution profiles were found to be dependent on the carrier and impregnation method used. Most importantly, in all cases more controlled and delayed release was achieved with the bioaerogels compared to using pure esomeprazole.

scholarly journals Aspirin-Loaded Polymeric Films for Drug Delivery Systems: Comparison between Soaking and Supercritical CO2 Impregnation

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 824
Author(s):  
Isabela Trindade Coutinho ◽  
Lígia Passos Maia-Obi ◽  
Mathilde Champeau
Keyword(s):  
Supercritical Co2 ◽  
Polymeric Films ◽  
Burst Release ◽  
Impregnation Method ◽  
Residual Solvent ◽  
Swelling Capacity ◽  
Polymeric Implants ◽  
Prolonged Drug Release ◽  
Concentration Limits

Polymeric implants loaded with drugs can overcome the disadvantages of oral or injection drug administration and deliver the drug locally. Several methods can load drugs into polymers. Herein, soaking and supercritical CO2 (scCO2) impregnation methods were employed to load aspirin into poly(l-lactic acid) (PLLA) and linear low-density polyethylene (LLDPE). Higher drug loadings (DL) were achieved with scCO2 impregnation compared to soaking and in a shorter time (3.4 ± 0.8 vs. 1.3 ± 0.4% for PLLA; and 0.4 ± 0.5 vs. 0.6 ± 0.5% for LLDPE), due to the higher swelling capacity of CO2. The higher affinity of aspirin explained the higher DL in PLLA than in LLDPE. Residual solvent was detected in LLDPE prepared by soaking, but within the FDA concentration limits. The solvents used in both methods acted as plasticizers and increased PLLA crystallinity. PLLA impregnated with aspirin exhibited faster hydrolysis in vitro due to the catalytic effect of aspirin. Finally, PLLA impregnated by soaking showed a burst release because of aspirin crystals on the PLLA surface, and released 100% of aspirin within 60 days, whereas the PLLA prepared with scCO2 released 60% after 74 days by diffusion and PLLA erosion. Hence, the scCO2 impregnation method is adequate for higher aspirin loadings and prolonged drug release.

Imaging of lamination patterns of the adult human olfactory bulb and tract: In vitro comparison of standard- and high-resolution 3T MRI, and MR microscopy at 9.4T

NeuroImage ◽  
2012 ◽  
Vol 60 (3) ◽  
pp. 1662-1670 ◽  
Author(s):  
Hartmut P. Burmeister ◽  
Thomas Bitter ◽  
Patrick M. Heiler ◽  
Andrey Irintchev ◽  
Rosemarie Fröber ◽  
...  
Keyword(s):  
High Resolution ◽  
Olfactory Bulb ◽  
Mr Microscopy ◽  
3T Mri ◽  
Adult Human ◽  
In Vitro Comparison

scholarly journals DIFFERENTIATION OF NEURONAL TYPES AND SYNAPSES IN MYELINATING CULTURES OF MOUSE CEREBELLUM

1964 ◽  
Vol 22 (1) ◽  
pp. 259-279 ◽  
Author(s):  
Merrill K. Wolf
Keyword(s):  
Purkinje Cells ◽  
Silver Impregnation ◽  
Impregnation Method ◽  
Two Cultures ◽  
Central Nervous Tissue ◽  
Mouse Cerebellum ◽  
Neuronal Types ◽  
Synaptic Boutons

The Holmes silver impregnation method has made possible the recognition of multiple neuronal types and synapses in myelinating cultures of mouse cerebellum. Well stained large and medium-sized neurons are always found in small numbers near ependymal formations and are considered to be roof nuclear neurons. Neurons with poorly stained somas, abruptly demarked from intensely stained axons, are numerous and often are arranged in palisades. With prolonged maintenance in vitro these neurons develop some but not all of the features of mature Purkinje cells. A few small, densely stained, bipolar neurons, often with one process bifurcated, are found in dense regions of some cultures of newborn cerebellum. These neurons are commoner in cultures from cerebella of older mice. They closely resemble the immature granule cell in vivo. All the neuron types recognized in cultures are present in the initial explants; neurons differentiate further in vitro, but new neurons probably do not form. Synaptic boutons are found on somas and dendrites of many Purkinje cells. Two cultures contained structures resembling the basket endings which surround Purkinje cell somas in vivo. The complexity of neuronal relationships in cultures of central nervous tissue is emphasized.

In vitro MR microscopy of the hippocampus in Alzheimer's disease

Neurology ◽  
1993 ◽  
Vol 43 (1 Part 1) ◽  
pp. 145-145 ◽  
Author(s):  
C. T. Huesgen ◽  
P. C. Burger ◽  
B. J. Crain ◽  
G. A. Johnson
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Mr Microscopy

Supercritical Carbon Dioxide Assisted Impregnation of Molsidomine into Poly(DL-Lactide-co-Glycolide) for Sustained Release Applications

2007 ◽  
Vol 342-343 ◽  
pp. 501-504 ◽  
Author(s):  
Hullathy Subban Ganapathy ◽  
Min Hee Woo ◽  
Seong Soo Hong ◽  
Kwon Taek Lim
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Water Soluble ◽  
Porous Polymer ◽  
In Vitro Dissolution ◽  
Prolonged Release ◽  
Impregnation Method ◽  
Polymer Monoliths ◽  
Supercritical Carbon

Supercritical carbon dioxide (scCO2) was used as a processing medium for the fabrication of drug encapsulated poly(DL-lactide-co-glycolide) (PLGA) monoliths for their potential application in the controlled release of water soluble drugs. Exposure of PLGA to scCO2 leads to effective plasticization and liquefaction due to the high solubility and interaction of the scCO2 in the copolymer. By exploiting this property, it was demonstrated that prolonged release formulations of molsidomine, a peripheral nitrovasodilator used to treat angina pectoris, can be prepared by chemical solvent-free, scCO2 assisted drug impregnation method. The in-vitro dissolution studies revealed that the release rates of drug from the porous polymer monoliths containing different amount of the drug samples were significantly retarded due to encapsulation of molsidomine into the PLGA matrix.

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