scholarly journals In vivo “hot spot” MR imaging of neural stem cells using fluorinated nanoparticles

2008 ◽  
Vol 60 (6) ◽  
pp. 1506-1511 ◽  
Author(s):  
Jesús Ruiz-Cabello ◽  
Piotr Walczak ◽  
Dorota A. Kedziorek ◽  
Vadappuram P. Chacko ◽  
Anna H. Schmieder ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mr Imaging ◽  
Neural Stem Cells ◽  
Hot Spot ◽  
Fluorinated Nanoparticles

scholarly journals In Vivo Targeted MR Imaging of Endogenous Neural Stem Cells in Ischemic Stroke

Molecules ◽  
2016 ◽  
Vol 21 (9) ◽  
pp. 1143 ◽  
Author(s):  
Fang Zhang ◽  
Xiaohui Duan ◽  
Liejing Lu ◽  
Xiang Zhang ◽  
Xiaomei Zhong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ischemic Stroke ◽  
Mr Imaging ◽  
Neural Stem Cells ◽  
Endogenous Neural Stem Cells

scholarly journals In Vivo Targeted Magnetic Resonance Imaging of Endogenous Neural Stem Cells in the Adult Rodent Brain

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Xiao-Mei Zhong ◽  
Fang Zhang ◽  
Ming Yang ◽  
Xue-Hua Wen ◽  
Xiang Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mr Imaging ◽  
Neural Stem Cells ◽  
Molecular Probe ◽  
Mammalian Brain ◽  
Intraventricular Administration ◽  
In Vivo Monitoring ◽  
Rodent Brain ◽  
Endogenous Neural Stem Cells

Neural stem cells in the adult mammalian brain have a significant level of neurogenesis plasticity. In vivo monitoring of adult endogenous NSCs would be of great benefit to the understanding of the neurogenesis plasticity under normal and pathological conditions. Here we show the feasibility of in vivo targeted MR imaging of endogenous NSCs in adult mouse brain by intraventricular delivery of monoclonal anti-CD15 antibody conjugated superparamagnetic iron oxide nanoparticles. After intraventricular administration of these nanoparticles, the subpopulation of NSCs in the anterior subventricular zone and the beginning of the rostral migratory stream could be in situ labeled and were in vivo visualized with 7.0-T MR imaging during a period from 1 day to 7 days after the injection. Histology confirmed that the injected targeted nanoparticles were specifically bound to CD15 positive cells and their surrounding extracellular matrix. Our results suggest that in vivo targeted MR imaging of endogenous neural stem cells in adult rodent brain could be achieved by using anti-CD15-SPIONs as the molecular probe; and this targeting imaging strategy has the advantage of a rapid in vivo monitoring of the subpopulation of endogenous NSCs in adult brains.

scholarly journals DNA–gadolinium–gold nanoparticles for in vivo T1 MR imaging of transplanted human neural stem cells

Biomaterials ◽  
2016 ◽  
Vol 77 ◽  
pp. 291-306 ◽  
Author(s):  
Francesca J. Nicholls ◽  
Matthew W. Rotz ◽  
Harmanvir Ghuman ◽  
Keith W. MacRenaris ◽  
Thomas J. Meade ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gold Nanoparticles ◽  
Mr Imaging ◽  
Neural Stem Cells ◽  
Human Neural Stem Cells

scholarly journals A novel polymeric micelle used for in vivo MR imaging tracking of neural stem cells in acute ischemic stroke

RSC Advances ◽  
2017 ◽  
Vol 7 (25) ◽  
pp. 15041-15052 ◽  
Author(s):  
Liejing Lu ◽  
Yong Wang ◽  
Minghui Cao ◽  
Meiwei Chen ◽  
Bingling Lin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ischemic Stroke ◽  
Mr Imaging ◽  
Neural Stem Cells ◽  
Acute Ischemic Stroke ◽  
Cholic Acid ◽  
Polymeric Micelles ◽  
Polymeric Micelle ◽  
Biodegradable Poly

Novel cationic polymeric micelles based on biodegradable poly(aspartic acid-dimethylethanediamine)–lysine–cholic acid were synthesized for in vivo tracking therapeutic stem cells using MRI.

scholarly journals mRNA-Driven Generation of Transgene-Free Neural Stem Cells from Human Urine-Derived Cells

Cells ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 1043 ◽  
Author(s):  
Phil Jun Kang ◽  
Daryeon Son ◽  
Tae Hee Ko ◽  
Wonjun Hong ◽  
Wonjin Yun ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Human Urine ◽  
Neurological Disorders ◽  
Therapeutic Potential ◽  
Embryonic Stem ◽  
Global Gene Expression ◽  
Patient Specific ◽  
Human Neural Stem Cells

Human neural stem cells (NSCs) hold enormous promise for neurological disorders, typically requiring their expandable and differentiable properties for regeneration of damaged neural tissues. Despite the therapeutic potential of induced NSCs (iNSCs), a major challenge for clinical feasibility is the presence of integrated transgenes in the host genome, contributing to the risk for undesired genotoxicity and tumorigenesis. Here, we describe the advanced transgene-free generation of iNSCs from human urine-derived cells (HUCs) by combining a cocktail of defined small molecules with self-replicable mRNA delivery. The established iNSCs were completely transgene-free in their cytosol and genome and further resembled human embryonic stem cell-derived NSCs in the morphology, biological characteristics, global gene expression, and potential to differentiate into functional neurons, astrocytes, and oligodendrocytes. Moreover, iNSC colonies were observed within eight days under optimized conditions, and no teratomas formed in vivo, implying the absence of pluripotent cells. This study proposes an approach to generate transplantable iNSCs that can be broadly applied for neurological disorders in a safe, efficient, and patient-specific manner.

scholarly journals Dynamic integration of enteric neural stem cells in ex vivo organotypic colon cultures

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Georgina Navoly ◽  
Conor J. McCann
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Organotypic Culture ◽  
Ex Vivo ◽  
Donor Cell ◽  
Colonic Tissue ◽  
Cell Therapies ◽  
Enteric Ganglia ◽  
Donor Cells

AbstractEnteric neural stem cells (ENSC) have been identified as a possible treatment for enteric neuropathies. After in vivo transplantation, ENSC and their derivatives have been shown to engraft within colonic tissue, migrate and populate endogenous ganglia, and functionally integrate with the enteric nervous system. However, the mechanisms underlying the integration of donor ENSC, in recipient tissues, remain unclear. Therefore, we aimed to examine ENSC integration using an adapted ex vivo organotypic culture system. Donor ENSC were obtained from Wnt1cre/+;R26RYFP/YFP mice allowing specific labelling, selection and fate-mapping of cells. YFP+ neurospheres were transplanted to C57BL6/J (6–8-week-old) colonic tissue and maintained in organotypic culture for up to 21 days. We analysed and quantified donor cell integration within recipient tissues at 7, 14 and 21 days, along with assessing the structural and molecular consequences of ENSC integration. We found that organotypically cultured tissues were well preserved up to 21-days in ex vivo culture, which allowed for assessment of donor cell integration after transplantation. Donor ENSC-derived cells integrated across the colonic wall in a dynamic fashion, across a three-week period. Following transplantation, donor cells displayed two integrative patterns; longitudinal migration and medial invasion which allowed donor cells to populate colonic tissue. Moreover, significant remodelling of the intestinal ECM and musculature occurred upon transplantation, to facilitate donor cell integration within endogenous enteric ganglia. These results provide critical evidence on the timescale and mechanisms, which regulate donor ENSC integration, within recipient gut tissue, which are important considerations in the future clinical translation of stem cell therapies for enteric disease.

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