scholarly journals Assessment of lipids in skeletal muscle by high-resolution spectroscopic imaging using fat as the internal standard: Comparison with water referenced spectroscopy

2008 ◽  
Vol 59 (6) ◽  
pp. 1259-1265 ◽  
Author(s):  
J. Weis ◽  
L. Johansson ◽  
F. Ortiz-Nieto ◽  
H. Ahlström
Keyword(s):  
Skeletal Muscle ◽  
High Resolution ◽  
Internal Standard ◽  
Spectroscopic Imaging

How to sample the entire length of a single muscle fiber quick-frozen after electrical point stimulation for high resolution EM

1992 ◽  
Vol 50 (1) ◽  
pp. 776-777
Author(s):  
Joachim R. Sommer ◽  
Teresa High ◽  
Betty Scherer ◽  
Isaiah Taylor ◽  
Rashid Nassar
Keyword(s):  
Skeletal Muscle ◽  
High Resolution ◽  
Action Potential ◽  
Muscle Fiber ◽  
Freeze Fracture ◽  
Freeze Substitution ◽  
Electrical Field Stimulation ◽  
Skeletal Muscle Fiber ◽  
Freeze Dried ◽  
Quick Freezing

We have developed a model that allows the quick-freezing at known time intervals following electrical field stimulation of a single, intact frog skeletal muscle fiber isolated by sharp dissection. The preparation is used for studying high resolution morphology by freeze-substitution and freeze-fracture and for electron probe x-ray microanlysis of sudden calcium displacement from intracellular stores in freeze-dried cryosections, all in the same fiber. We now show the feasibility and instrumentation of new methodology for stimulating a single, intact skeletal muscle fiber at a point resulting in the propagation of an action potential, followed by quick-freezing with sub-millisecond temporal resolution after electrical stimulation, followed by multiple sampling of the frozen muscle fiber for freeze-substitution, freeze-fracture (not shown) and cryosectionmg. This model, at once serving as its own control and obviating consideration of variances between different fibers, frogs etc., is useful to investigate structural and topochemical alterations occurring in the wake of an action potential.

scholarly journals Isotope analysis of highly enriched “silicon-28” by high-resolution inductively coupled plasma mass spectrometry using an internal standard

2021 ◽  
Vol 25 (2) ◽  
pp. 98-109
Author(s):  
P. A. Otopkova ◽  
◽  
A. M. Potapov ◽  
A. I. Suchkov ◽  
A. D. Bulanov ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Matrix Element ◽  
High Resolution ◽  
Inductively Coupled Plasma ◽  
Internal Standard ◽  
Isotopic Analysis ◽  
Random Component ◽  
Inductively Coupled ◽  
The Matrix ◽  
Plasma Mass Spectrometry

In order to study the isotopic effects in semiconductor materials, single crystals of high chemical and isotopic purity are required. The reliability of the obtained data on the magnitude and the direction of isotopic shifts depends on the accuracy of determining the concentration of all stable isotopes. In the isotopic analysis of enriched “silicon-28” with a high degree of enrichment (> 99.99%), it is necessary to determine the impurities of 29Si and 30Si isotopes at the level of 10-3 ¸ 10-5 at. %. At this concentration level, these isotopes can be considered as impurities. It is difficult to achieve high measurement accuracy with simultaneous registration of the main and “impurity” isotopes in such a wide range of concentrations. The registration of analytical signals of silicon isotopes must be carried out in the solutions with different matrix concentrations. The use of the solutions with the high concentration of the matrix element requires the introduction of corrections for matrix noise and the drift of the instrument sensitivity during the measurement. It is possible to reduce the influence of the irreversible non-spectral interference and sensitivity drift by using the method of internal standardization. The inconsistency of the literature data on the selection criteria for the internal standard required studying the behavior of the signals of the “candidates for the internal standard” for the ELEMENT 2 single-collector high-resolution inductively coupled plasma mass spectrometer on the matrix element concentration and the nature of the solvent, as well as on the solution nebulizing time. Accounting for the irreversible non-spectral matrix noise and instrumental drift in isotopic analysis of enriched “silicon-28” and initial 28SiF4 by inductively coupled plasma mass spectrometry had allowed us to reduce by 3-5 times the random component and by more than an order of magnitude the systematic component of the measurement error in comparison with the external standard method. This made it possible to carry out, with sufficient accuracy, the operational control of the isotopic composition of enriched “silicon-28”, both in the form of silicon tetrafluoride and polycrystalline silicon obtained from it, using a single serial device in the range of isotopic concentrations 0.0001–99.999%.

Immunoadsorption to improve gas chromatography/high-resolution mass spectrometry of estradiol-17 beta in plasma.

1983 ◽  
Vol 29 (4) ◽  
pp. 677-680 ◽  
Author(s):  
S J Gaskell ◽  
B G Brownsey
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
Trimethylsilyl Ether ◽  
Internal Standard ◽  
Selected Ion Monitoring ◽  
High Resolution Mass ◽  
Precise Quantification ◽  
Resolution Mass

Abstract We describe a new, highly selective procedure for the determination of estradiol-17 beta in plasma. Samples are extracted with a micro-cellulose-coupled antiserum to estradiol-17 beta. Conversion of the extracted steroid to the bis(trimethylsilyl) ether is followed by gas chromatography/high-resolution mass spectrometry with selected ion monitoring. Precise quantification is achieved through the use of [2H3]estradiol-17 beta as internal standard.

High-resolution Spectroscopic Imaging of Counter-streaming Motions in Solar Active Region Magnetic Loops

2019 ◽  
Vol 881 (1) ◽  
pp. L25
Author(s):  
Xu Yang ◽  
Wenda Cao ◽  
Haisheng Ji ◽  
Parida Hashim ◽  
Jinhua Shen
Keyword(s):  
High Resolution ◽  
Active Region ◽  
Spectroscopic Imaging ◽  
Solar Active Region

Radiofrequency configuration to facilitate bilateral breast31P MR spectroscopic imaging and high-resolution MRI at 7 Tesla

2014 ◽  
Vol 74 (6) ◽  
pp. 1803-1810 ◽  
Author(s):  
Tijl A. van der Velden ◽  
Michel Italiaander ◽  
Wybe J.M. van der Kemp ◽  
Alexander J.E. Raaijmakers ◽  
A.M. Th. Schmitz ◽  
...  
Keyword(s):  
High Resolution ◽  
Spectroscopic Imaging ◽  
7 Tesla ◽  
High Resolution Mri ◽  
Mr Spectroscopic Imaging

High-Resolution Metabolic Mapping of the Cerebellum Using a Zoomed Magnetic Resonance Spectroscopic Imaging

2020 ◽  
Author(s):  
Uzay E. Emir ◽  
Jaiyta Sood ◽  
Mark Chiew ◽  
Albert Thomas ◽  
Sean P. Lane
Keyword(s):  
Magnetic Resonance ◽  
High Resolution ◽  
Region Of Interest ◽  
Spectroscopic Imaging ◽  
Magnetic Resonance Spectroscopic Imaging ◽  
Total N ◽  
Group Averaging ◽  
Full Intensity ◽  
Nominal Resolution ◽  
Very High

AbstractPurposeThe human cerebellum plays an important role in functional activity cerebrum which is ranging from motor to cognitive activities since due to its relaying role between spinal cord and cerebrum. The cerebellum poses many challenges to magnetic resonance spectroscopic imaging (MRSI) due to the caudal location, the susceptibility to physiological artifacts and partial volume artifact due to its complex anatomical structure. Thus, in present study, we propose a high-resolution MRSI acquisition scheme for the cerebellum.MethodsA zoomed or reduced-field of view (rFOV) metabolite-cycled full-intensity magnetic resonance spectroscopic imaging (MRSI) at 3T with a nominal resolution of 62.5 μL was developed. Single-slice rFOV MRSI data were acquired from the cerebellum of 5 healthy subjects with a nominal resolution of 2.5□×□2.5□mm2 in 9□minutes 36. Spectra were quantified with LCModel. A spatially unbiased atlas template of the cerebellum was used for analyzing metabolite distributions in the cerebellum.ResultsThe high quality of the achieved spectra enabled to generate a high-resolution metabolic map of total N-acetylaspartate, total creatine, total choline, glutamate+glutamine and myo-inositol with Cramér-Rao lower bounds below 50%. A spatially unbiased atlas template of the cerebellum-based region of interest (ROIs) analysis resulted in spatially dependent metabolite distributions in 9 ROIs. The group-averaging across subjects in the Montreal Neurological Institute-152 template space allowed to generate a very high-resolution metabolite maps in the cerebellum.ConclusionThese findings indicate that very high-resolution metabolite probing of cerebellum is feasible using rFOV or zoomed MRSI at 3T.

High-resolution spectroscopic imaging of the Sun with a universal birefringent filter and a Fabry-Perot Interferometer

1992 ◽  
Vol 15 (5) ◽  
pp. 509-518 ◽  
Author(s):  
F. Cavallini ◽  
G. Ceppatelli ◽  
A. Righini ◽  
F. Berrilli ◽  
B. Caccin ◽  
...  
Keyword(s):  
High Resolution ◽  
Spectroscopic Imaging ◽  
The Sun ◽  
Birefringent Filter ◽  
Fabry Perot
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