scholarly journals Modeling oxygen effects in tissue-preparation neuronal-current MRI

2007 ◽  
Vol 58 (2) ◽  
pp. 407-412 ◽  
Author(s):  
Qingfei Luo ◽  
Ho-Ling Liu ◽  
Brent Parris ◽  
Huo Lu ◽  
David M. Senseman ◽  
...  
Keyword(s):  
Tissue Preparation ◽  
Oxygen Effects ◽  
Neuronal Current Mri ◽  
Neuronal Current

Neuronal Current MRI without BOLD Contamination

NeuroImage ◽  
2009 ◽  
Vol 47 ◽  
pp. S185 ◽  
Author(s):  
Q Luo ◽  
J-H Gao
Keyword(s):  
Neuronal Current Mri ◽  
Neuronal Current

scholarly journals Modeling neuronal current MRI signal with human neuron

2011 ◽  
Vol 65 (6) ◽  
pp. 1680-1689 ◽  
Author(s):  
Qingfei Luo ◽  
Xia Jiang ◽  
Bin Chen ◽  
Yi Zhu ◽  
Jia-Hong Gao
Keyword(s):  
Neuronal Current Mri ◽  
Human Neuron ◽  
Neuronal Current

scholarly journals Detection of neuronal current MRI in human without BOLD contamination

2011 ◽  
Vol 66 (2) ◽  
pp. 492-497 ◽  
Author(s):  
Qingfei Luo ◽  
Xia Jiang ◽  
Jia-Hong Gao
Keyword(s):  
Neuronal Current Mri ◽  
Neuronal Current

scholarly journals Physiologically evoked neuronal current MRI in a bloodless turtle brain: Detectable or not?

NeuroImage ◽  
2009 ◽  
Vol 47 (4) ◽  
pp. 1268-1276 ◽  
Author(s):  
Qingfei Luo ◽  
Huo Lu ◽  
Hanbing Lu ◽  
David Senseman ◽  
Keith Worsley ◽  
...  
Keyword(s):  
Neuronal Current Mri ◽  
Neuronal Current

Computational Modeling of Neuronal Current MRI Signals with Rat Somatosensory Cortical Neurons

2015 ◽  
Vol 8 (3) ◽  
pp. 253-262 ◽  
Author(s):  
Seyed Mehdi BagheriMofidi ◽  
Majid Pouladian ◽  
Seyed Behnammodin Jameie ◽  
Ali Abbaspour Tehrani-Fard
Keyword(s):  
Computational Modeling ◽  
Cortical Neurons ◽  
Neuronal Current Mri ◽  
Neuronal Current

Localization of pancreatic enzymes in ultrathin frozen sections stained with metal labeled antibody

1978 ◽  
Vol 36 (2) ◽  
pp. 90-91
Author(s):  
Kenjiro Yasuda
Keyword(s):  
Fluorescent Antibody ◽  
Pancreatic Enzymes ◽  
Tissue Preparation ◽  
Zymogen Granules ◽  
Frozen Sections ◽  
En Bloc ◽  
Antibody Method ◽  
Ultrathin Frozen Sections ◽  
Fluorescent Antibody Method

Localization of amylase,chymotrypsinogen and trypsinogen in pancreas was demonstrated by Yasuda and Coons (1966), by using fluorescent antibody method. These enzymes were naturally found in the zymogen granules. Among them, amylase showed a diffuse localization around the nucleus, in addition to the zymogen granules. Using ferritin antibody method, scattered ferritin granules were also found around the Golgi area (Yasuda et al.,1967). The recent advance in the tissue preparation enables the antigen to be localized in the ultrathin frozen sections, by applying the labeled antibodies onto the sections instead of staining the tissue en bloc.The present study deals with the comparison of the localization of amylase and lipase demonstrated by applying the bismuth-labeled, peroxidase-labeled and ferritin-labeled antibody methods on the ultrathin frozen sections of pancreas, and on the blocks of the same tissue.

Effects of Hyperoxia on Lung Utrastructure

1970 ◽  
Vol 28 ◽  
pp. 26-27
Author(s):  
Gladys Harrison
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Light Microscopy ◽  
Oxygen Effects ◽  
Age Dependent ◽  
The Central Nervous System ◽  
The Subject ◽  
Space Age ◽  
Alveolar Septa ◽  
Extensive Damage

With the advent of the space age and the need to determine the requirements for a space cabin atmosphere, oxygen effects came into increased importance, even though these effects have been the subject of continuous research for many years. In fact, Priestly initiated oxygen research when in 1775 he published his results of isolating oxygen and described the effects of breathing it on himself and two mice, the only creatures to have had the “privilege” of breathing this “pure air”.Early studies had demonstrated the central nervous system effects at pressures above one atmosphere. Light microscopy revealed extensive damage to the lungs at one atmosphere. These changes which included perivascular and peribronchial edema, focal hemorrhage, rupture of the alveolar septa, and widespread edema, resulted in death of the animal in less than one week. The severity of the symptoms differed between species and was age dependent, with young animals being more resistant.

DNA nick end labeling: a reliable marker for apoptosis?

1995 ◽  
Vol 53 ◽  
pp. 962-963
Author(s):  
Anne F. Bushnell ◽  
Sarah Webster ◽  
Lynn S. Perlmutter
Keyword(s):  
Cell Death ◽  
Cultured Cells ◽  
Apoptotic Cell ◽  
Morphological Characteristics ◽  
Detection Methods ◽  
Tissue Preparation ◽  
Preparation Methods ◽  
Dna Laddering ◽  
Disease States ◽  
Reliable Marker

Apoptosis, or programmed cell death, is an important mechanism in development and in diverse disease states. The morphological characteristics of apoptosis were first identified using the electron microscope. Since then, DNA laddering on agarose gels was found to correlate well with apoptotic cell death in cultured cells of dissimilar origins. Recently numerous DNA nick end labeling methods have been developed in an attempt to visualize, at the light microscopic level, the apoptotic cells responsible for DNA laddering.The present studies were designed to compare various tissue processing techniques and staining methods to assess the occurrence of apoptosis in post mortem tissue from Alzheimer's diseased (AD) and control human brains by DNA nick end labeling methods. Three tissue preparation methods and two commercial DNA nick end labeling kits were evaluated: the Apoptag kit from Oncor and the Biotin-21 dUTP 3' end labeling kit from Clontech. The detection methods of the two kits differed in that the Oncor kit used digoxigenin dUTP and anti-digoxigenin-peroxidase and the Clontech used biotinylated dUTP and avidinperoxidase. Both used 3-3' diaminobenzidine (DAB) for final color development.

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