Validation of13c nmr measurement of human skeletal muscle glycogen by direct biochemical assay of needle biopsy samples

1992 ◽  
Vol 27 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Roy Taylor ◽  
Thomas B. Price ◽  
Douglas L. Rothman ◽  
Robert G. Shulman ◽  
Gerald I. Shulman
Keyword(s):  
Skeletal Muscle ◽  
Muscle Glycogen ◽  
Needle Biopsy ◽  
Human Skeletal Muscle ◽  
Biochemical Assay ◽  
Skeletal Muscle Glycogen

Human Skeletal Muscle Glycogen Synthetase Activities with Exercise and Training

1972 ◽  
Vol 50 (5) ◽  
pp. 411-415 ◽  
Author(s):  
A. W. Taylor ◽  
R. Thayer ◽  
S. Rao
Keyword(s):  
Skeletal Muscle ◽  
Muscle Glycogen ◽  
Human Skeletal Muscle ◽  
Human Subjects ◽  
Fitness Level ◽  
Glycogen Synthetase ◽  
Skeletal Muscle Glycogen ◽  
Level Of Activity ◽  
Exercise And Training ◽  
And Training

A 5 month training program increased skeletal muscle glycogen synthetase activities of both the 'I' and 'D' forms in human subjects. The level of activity of the enzyme appears to be directly related to the physical fitness level of the subjects tested.

scholarly journals Human skeletal muscle glycogen utilization in exhaustive exercise: role of subcellular localization and fibre type

2011 ◽  
Vol 589 (11) ◽  
pp. 2871-2885 ◽  
Author(s):  
Joachim Nielsen ◽  
Hans-Christer Holmberg ◽  
Henrik D. Schrøder ◽  
Bengt Saltin ◽  
Niels Ørtenblad
Keyword(s):  
Skeletal Muscle ◽  
Subcellular Localization ◽  
Muscle Glycogen ◽  
Fibre Type ◽  
Human Skeletal Muscle ◽  
Exhaustive Exercise ◽  
Skeletal Muscle Glycogen ◽  
Glycogen Utilization

scholarly journals Effects of Oral Resveratrol Supplementation on Glycogen Replenishment and Mitochondria Biogenesis in Exercised Human Skeletal Muscle

Nutrients ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3721
Author(s):  
Chun-Ching Huang ◽  
Chia-Chen Liu ◽  
Jung-Piao Tsao ◽  
Chin-Lin Hsu ◽  
I-Shiung Cheng
Keyword(s):  
Skeletal Muscle ◽  
Glucose Uptake ◽  
Muscle Glycogen ◽  
Human Skeletal Muscle ◽  
Young Male ◽  
Gene Expressions ◽  
Plasma Parameters ◽  
Post Exercise ◽  
Glycogen Resynthesis ◽  
Mitochondria Biogenesis

The present study aimed to investigate the effect of oral resveratrol supplementation on the key molecular gene expressions involved in mitochondria biogenesis and glycogen resynthesis in human skeletal muscle. Nine young male athletes participated in the single-blind and crossover designed study. All subjects completed a 4-day resveratrol and placebo supplement in a randomized order while performing a single bout of cycling exercise. Immediately after the exercise challenge, the subjects consumed a carbohydrate (CHO) meal (2 g CHO/Kg body mass) with either resveratrol or placebo capsules. Biopsied muscle samples, blood samples and expired gas samples were obtained at 0 h and 3 h after exercise. The muscle samples were measured for gene transcription factor expression by real-time PCR for glucose uptake and mitochondria biogenesis. Plasma glucose, insulin, glycerol, non-esterified fatty acid concentrations and respiratory exchange ratio were analyzed during post-exercise recovery periods. The results showed that the muscle glycogen concentrations were higher at 3 h than at 0 h; however, there were no difference between resveratrol trial and placebo trial. There were no significantly different concentrations in plasma parameters between the two trials. Similarly, no measured gene expressions were significant between the two trials. The evidence concluded that the 4-day oral resveratrol supplementation did not improve post-exercise muscle glycogen resynthesis and related glucose uptake and mitochondrial biosynthesis gene expression in men.

Sign in / Sign up

Export Citation Format

Share Document