Signal loss induced by superparamagnetic iron oxide particle in NMR spin-echo images: The role of diffusion

1990 ◽  
Vol 14 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Yoseph Rozenman ◽  
Xueming Zou ◽  
Howard L. Kantor
Keyword(s):  
Iron Oxide ◽  
Spin Echo ◽  
Oxide Particle ◽  
Superparamagnetic Iron Oxide ◽  
Iron Oxide Particle ◽  
Signal Loss

Superparamagnetic Iron Oxide Particle-Enhanced Hepatic MRI

2002 ◽  
Vol 9 (2) ◽  
pp. S473-S475
Author(s):  
Ryohei Kuwatsuru ◽  
Syogo Ito ◽  
Masayuki Akamatsu ◽  
Tadayuki Maehara
Keyword(s):  
Iron Oxide ◽  
Oxide Particle ◽  
Superparamagnetic Iron Oxide ◽  
Iron Oxide Particle

Non-invasive detection of liver fibrosis: Is superparamagnetic iron oxide particle-enhanced MR imaging a contributive technique?

2003 ◽  
Vol 13 (3) ◽  
pp. 467-474 ◽  
Author(s):  
Olivier Lucidarme ◽  
Florence Baleston ◽  
Mehdi Cadi ◽  
Marie-France Bellin ◽  
Frédéric Charlotte ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Liver Fibrosis ◽  
Mr Imaging ◽  
Oxide Particle ◽  
Superparamagnetic Iron Oxide ◽  
Iron Oxide Particle ◽  
Non Invasive

Efficient and Rapid Labeling of Transplanted Cell Populations with Superparamagnetic Iron Oxide Nanoparticles Using Cell Surface Chemical Biotinylation for in Vivo Monitoring by MRI

2010 ◽  
Vol 19 (4) ◽  
pp. 419-429 ◽  
Author(s):  
Po-Wah So ◽  
Tammy Kalber ◽  
David Hunt ◽  
Michael Farquharson ◽  
Alia Al-Ebraheem ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cell Tracking ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Cell Populations ◽  
Oxide Nanoparticles ◽  
Signal Loss

Determination of the dynamics of specific cell populations in vivo is essential for the development of cell-based therapies. For cell tracking by magnetic resonance imaging (MRI), cells need to internalize, or be surface labeled with a MRI contrast agent, such as superparamagnetic iron oxide nanoparticles (SPIOs): SPIOs give rise to signal loss by gradient-echo and T2-weighted MRI techniques. In this study, cancer cells were chemically tagged with biotin and then magnetically labeled with anti-biotin SPIOs. No significant detrimental effects on cell viability or death were observed following cell biotinylation. SPIO-labeled cells exhibited signal loss compared to non-SPIO-labeled cells by MRI in vitro. Consistent with the in vitro MRI data, signal attenuation was observed in vivo from SPIO-labeled cells injected into the muscle of the hind legs, or implanted subcutaneously into the flanks of mice, correlating with iron detection by histochemical and X-ray fluorescence (XRF) methods. To further validate this approach, human mesenchymal stem cells (hMSCs) were also employed. Chemical biotinylation and SPIO labeling of hMSCs were confirmed by fluorescence microscopy and flow cytometry. The procedure did not affect proliferation and multipotentiality, or lead to increased cell death. The SPIO-labeled hMSCs were shown to exhibit MRI signal reduction in vitro and was detectable in an in vivo model. In this study, we demonstrate a rapid, robust, and generic methodology that may be a useful and practical adjuvant to existing methods of cell labeling for in vivo monitoring by MRI. Further, we have shown the first application of XRF to provide iron maps to validate MRI data in SPIO-labeled cell tracking studies.

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