In vivo evaluation of intracellular pH and high-energy phosphate metabolities during regional myocardial ischemia in cats using31P nuclear magnetic resonance

1986 ◽  
Vol 3 (2) ◽  
pp. 262-269 ◽  
Author(s):  
Paul D. Stein ◽  
Sidney Goldstein ◽  
Hani N. Sabbah ◽  
Zhi-Quan Liu ◽  
Joseph A. Helpern ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Myocardial Ischemia ◽  
Intracellular Ph ◽  
High Energy ◽  
High Energy Phosphate ◽  
In Vivo Evaluation ◽  
Regional Myocardial Ischemia ◽  
Nuclear Magnetic

31P-Nuclear magnetic resonance spectroscopy of rat pituitary tumours in vivo

1985 ◽  
Vol 106 (3) ◽  
pp. 349-353 ◽  
Author(s):  
R. A. Prysor-Jones ◽  
J. J. Silverlight ◽  
J. S. Jenkins ◽  
A. N. Stevens ◽  
J. L. Rodrigues ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Intracellular Ph ◽  
High Energy ◽  
Prolactin Secretion ◽  
Plasma Prolactin ◽  
Pituitary Tumours ◽  
Phosphorus Metabolites ◽  
Nuclear Magnetic

ABSTRACT 31P-Nuclear magnetic resonance (NMR) spectra were obtained in the living rat from 19 implanted prolactin-secreted pituitary tumours. Seven major peaks were found including those arising from the high energy phosphorus metabolites ATP and phosphocreatine. Intracellular pH of the tumours was measured and a relationship with prolactin secretion was observed, the highest plasma prolactin concentrations being associated with an intracellular pH >7·18. Repeated NMR measurements in three tumours over periods of up to 21 days revealed progressive changes with age, shown by an increase in inorganic phosphate, a decrease in high energy phosphorus metabolites and a decrease in prolactin secretion. It is concluded that NMR spectroscopy provides a useful method of studying intracellular events which accompany hormone secretion in vivo. J. Endocr. (1985) 106, 349–353

Hepatic phosphate trapping, decreased ATP, and increased feeding after 2,5-anhydro-D-mannitol

1994 ◽  
Vol 266 (1) ◽  
pp. R112-R117 ◽  
Author(s):  
N. E. Rawson ◽  
H. Blum ◽  
M. D. Osbakken ◽  
M. I. Friedman
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Time Course ◽  
Time Frame ◽  
High Energy ◽  
Resonance Spectroscopy ◽  
Phosphate Diesters ◽  
Layer Chromatography ◽  
Nuclear Magnetic

The mechanism by which the fructose analogue 2,5-anhydro-D-mannitol (2,5-AM) elicits feeding behavior was investigated by studying its metabolism and biochemical effects in liver. Thin-layer chromatography of liver extracts from rats given 2,5-AM containing 14C-labeled 2,5-AM showed that the analogue is phosphorylated in vivo with a time course that parallels the eating response. In vivo 31P nuclear magnetic resonance spectroscopy of rat liver during intravenous infusion of 2,5-AM and high-resolution nuclear magnetic resonance analyses of liver extracts showed that 2,5-AM is rapidly phosphorylated in liver, trapping hepatic phosphate and decreasing ATP, inorganic phosphate, and phosphate diesters. These changes occurred in a time frame in which the feeding response is elicited in conscious animals given the same dose of 2,5-AM by the same route. During an interval in which 2,5-AM increased eating, it also increased urinary uric acid excretion, implicating enhanced adenosine degradation in the reduction in hepatic ATP. These results provide the first direct evidence that changes in a high-energy phosphate-carrying compound in liver may provide a signal to initiate eating behavior.

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