Effects of increased scrotal temperature on semen quality and seminal plasma proteins in Brahman bulls

2020 ◽  
Vol 87 (5) ◽  
pp. 574-597
Author(s):  
Gry Brandt Boe‐Hansen ◽  
João Paulo A. Rêgo ◽  
Nana Satake ◽  
Bronwyn Venus ◽  
Pawel Sadowski ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Semen Quality ◽  
Scrotal Temperature ◽  
Seminal Plasma Proteins

Analysis of Seminal Plasma Proteins of South Indian Jersey and Hybrid Bulls and their Correlation with Semen Quality

2015 ◽  
Vol 10 (1) ◽  
pp. 92-98 ◽  
Author(s):  
A.S. Vickram ◽  
V. Devi Rajeswari ◽  
M. Ramesh Pathy ◽  
T.B. Sridharan
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Semen Quality ◽  
South Indian ◽  
Seminal Plasma Proteins

310 SEASON INFLUENCE UPON SEMINAL PLASMA PROTEINS IN BROWN SWISS BULLS

2010 ◽  
Vol 22 (1) ◽  
pp. 311
Author(s):  
M. G. M. Chacur ◽  
F. P. Sirchia ◽  
A. C. L. Ruiz ◽  
M. L. Guaberto
Keyword(s):  
New York ◽  
Seminal Plasma ◽  
Plasma Proteins ◽  
Protein Quality ◽  
Semen Quality ◽  
Reproductive Organs ◽  
Low Fertility ◽  
Significance Level ◽  
Brown Swiss ◽  
Seminal Plasma Proteins

Seminal plasma is a complex of secretions of the male accessory reproductive organs and appears to exert important effects on sperm function (Shivaji et al. 1990 Proteins of Seminal Plasma, Wiley, New York, NY, USA). The protein quality of the seminal plasma may affect positively the bulls’fertility (Killiam et al. 1993 Biol. Reprod. 49, 1202-1207). Peptides of 55 and 66 kDa were present in bulls with excellent spermatic conditions for example motility and vigor. On the other hand, 16- and 36-kDa peptides were observed with unfavorable spermatic conditions (Chacur et al. 2009 Anim. Reprod. 6, 339). The objective was to determine the influence of season on seminal plasma proteins in Brown Swiss bulls. Semen from 33 Brown Swiss bulls 24 months of age was collected by electroejaculation during winter (from June to August) and summer (from December to February) in the southern hemisphere in 2008. Semen samples were collected with 14-day intervals totalizing 196 ejaculates. Samples of semen were centrifuged (1500g/15 min) and the seminal plasma was conditioned in cryotubes and stored at -20°C until further processing. Proteins were extracted from 200 μL of each sample in 2 mL of extraction buffer composed of 0.625 M Tris-HCl, at pH 6.8, in 2% SDS, 5% fi-mercaptoethanol, and 20% of glycerol. Percentages of different plasma proteins by season were statistically compared by the chi-square test with significance level at P < 0.05). Proteins were quantified according to Bradford (1976 Anal. Biochem. 72, 248-254) and electrophoresis was performed according to Laemmili (1970 Nature 227, 680-685). Gels were fixed with isopropanol: acetic acid: water (4:1:5 v/v) for 30 minutes and stained in the same solution with 2% of Coomassie Blue R250. In 26 bulls, the absence of high molecular weight (HMW; 55 kDa, 66 kDa, and 80 kDa) proteins was found in the summer. There was a significant increase (P < 0.05) in total spermatic defects, acrosome defects, and distal cytoplasmatic droplets in these bulls. The 40-kDa protein that reflected low fertility was observed in 10 bulls in the summer with semen quality decreases. The 11 bulls showed presence of HMW (55 kDa) in the winter. In 11 bulls, HMW (55 kDa, 66 kDa, or 80 kDa) proteins were present with a satisfactory semen condition according to Killiam et al. (1993 Biol. Reprod. 49, 1202-1207). In conclusion, the seasons of the year may influence the presence of proteins in seminal plasma. There was a direct relationship of the season with seminal plasma proteins. The presence of the proteins of 20 kDa, 55 kDa, 66 kDa, and 80 kDa suggested an increase of the semen quality during the winter. UNOESTE and Bartira Farm.

Genetic Polymorphism of Boar Seminal Plasma Proteins

1993 ◽  
Vol 64 (3) ◽  
pp. 221-227
Author(s):  
Soichi TSUJI ◽  
Masatoshi ASAO ◽  
Hiroshi KUSUNOKI ◽  
Takao OISHI
Keyword(s):  
Genetic Polymorphism ◽  
Seminal Plasma ◽  
Plasma Proteins ◽  
Seminal Plasma Proteins

Seasonal differences in seminal plasma proteins from two bovine breeds adapted to a subtropical climate

2021 ◽  
Vol 53 (1) ◽  
Author(s):  
Paula Lorena Souto ◽  
Lilian Silveira Travassos Carmouy ◽  
Cristiane Santos ◽  
Edison Martins ◽  
Vera Martins ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Subtropical Climate ◽  
Seasonal Differences ◽  
Seminal Plasma Proteins

scholarly journals Seminal Plasma Proteins Revert the Cold-Shock Damage on Ram Sperm Membrane1

2000 ◽  
Vol 63 (5) ◽  
pp. 1531-1537 ◽  
Author(s):  
Beatriz Barrios ◽  
Rosaura Pérez-Pé ◽  
Margarita Gallego ◽  
Agustín Tato ◽  
Jesús Osada ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Cold Shock ◽  
Seminal Plasma Proteins ◽  
Ram Sperm

Proteomic approach for purification of seminal plasma proteins involved in tumor proliferation

2007 ◽  
Vol 30 (12) ◽  
pp. 1979-1988 ◽  
Author(s):  
Md Imtaiyaz Hassan ◽  
Vijay Kumar ◽  
Tara Kashav ◽  
Neelima Alam ◽  
Tej P. Singh ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Tumor Proliferation ◽  
Proteomic Approach ◽  
Seminal Plasma Proteins
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