Analyses of mRNA‐seq and miRNA‐seq of the brain reveal the sex differences of gene expression and regulation before and during gonadal differentiation in 17β‐estradiol or 17α‐methyltestosterone‐induced olive flounder ( Paralichthys olivaceus )

2019 ◽  
Vol 87 (1) ◽  
pp. 78-90
Author(s):  
Yuxia Zou ◽  
Zhihao Wu ◽  
Zhaofei Fan ◽  
Dongdong Liang ◽  
Lijuan Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Sex Differences ◽  
Paralichthys Olivaceus ◽  
Olive Flounder ◽  
Gonadal Differentiation ◽  
Gene Expression And Regulation ◽  
17Β Estradiol ◽  
The Brain ◽  
Olive Flounder Paralichthys Olivaceus

scholarly journals EST-based Identification of Genes Expressed in the Brain of the Olive Flounder Paralichthys olivaceus

2009 ◽  
Vol 12 (4) ◽  
pp. 286-292
Author(s):  
Jeong-Ho Lee ◽  
Jae-Koo Noh ◽  
Hyun-Chul Kim ◽  
Choul-Ji Park ◽  
Byung-Hwa Min ◽  
...  
Keyword(s):  
Paralichthys Olivaceus ◽  
Olive Flounder ◽  
The Brain ◽  
Olive Flounder Paralichthys Olivaceus

Sexually dimorphic gene expression patterns during gonadal differentiation in olive flounder, Paralichthys olivaceus

2015 ◽  
Vol 65 (3-4) ◽  
pp. 193-207 ◽  
Author(s):  
Aiyun Wen ◽  
Feng You ◽  
Peng Sun ◽  
Jun Li ◽  
Dongdong Xu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Synergistic Effects ◽  
Paralichthys Olivaceus ◽  
Expression Patterns ◽  
Olive Flounder ◽  
Gene Expression Patterns ◽  
Gonadal Differentiation ◽  
Rt Pcr ◽  
Related Transcription Factor

The present study aims to elucidate the different expression patterns and possible roles of Doublesex and Mab-3-related transcription factor 1 (dmrt1), dmrt4, SRY-related transcription factor 9 (sox9) and cytochrome P450 aromatase 19a (cyp19a) during gonadal differentiation in olive flounder, Paralichthys olivaceus. We first analyzed the gene expression patterns in tissues using RT-PCR, which indicated dmrt1, sox9 and cyp19a were sex-related genes with sexual dimorphic expression. The quantitative expression changes of these three genes together with dmrt4 during gonadal differentiation were further examined using real-time RT-PCR. The results showed that dmrt1 was scarcely expressed in the primitive gonad and during following periods of gonadal differentiation. Its expression increased rapidly in the differentiating testis. Dmrt4 was strongly expressed in primitive gonads and much less expressed during following periods of gonadal differentiation. Its expression became strong in differentiating testes. While sox9 was highly expressed in the primitive gonad, it was expressed with fluctuations during following periods of gonadal differentiation. Cyp19a started expressing in primitive gonads, and its expression quantity fluctuated during latter periods of gonadal differentiation, but was strongly expressed in the early stage of differentiating ovaries. Results of in situ hybridization showed that dmrt4 and sox9 transcripts were both mainly localized in spermatocytes and our results suggested these four sex-related genes might be involved in gonadal differentiation through their synergistic effects in flounder.

scholarly journals Investigation of gene expression in primary embryonic cell line (FGBC8) from olive flounder Paralichthys olivaceus

2021 ◽  
Vol 24 (11) ◽  
pp. 370-374
Author(s):  
Ju-Won Kim ◽  
Ja Young Cho ◽  
Won-Kyong Chun ◽  
Dong-Gyun Kim ◽  
Bo-Hye Nam ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Paralichthys Olivaceus ◽  
Embryonic Cell ◽  
Olive Flounder ◽  
Embryonic Cell Line ◽  
Olive Flounder Paralichthys Olivaceus
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