Porcine IVF embryo development and estrogen receptors are influenced by the concentration of percoll gradients during sperm selection

2019 ◽  
Vol 87 (1) ◽  
pp. 135-141
Author(s):  
Lain U. Ohlweiler ◽  
Joana C. Mezzalira ◽  
Alceu Mezzalira
Keyword(s):  
Estrogen Receptors ◽  
Embryo Development ◽  
Sperm Selection ◽  
Percoll Gradients

Sperm selection method enhances embryo development.

2001 ◽  
Vol 76 (3) ◽  
pp. S74
Author(s):  
J.M. Nani ◽  
J.G. Brasch ◽  
R. Summers ◽  
R. Springer ◽  
R. Jeyendran
Keyword(s):  
Embryo Development ◽  
Selection Method ◽  
Sperm Selection

scholarly journals Sperm selection by rheotaxis improves sperm quality and early embryo development

Reproduction ◽  
2021 ◽  
Vol 161 (3) ◽  
pp. 343-352 ◽  
Author(s):  
Jon Romero-Aguirregomezcorta ◽  
Ricardo Laguna-Barraza ◽  
Raúl Fernández-González ◽  
Miriama Štiavnická ◽  
Fabian Ward ◽  
...  
Keyword(s):  
Membrane Fluidity ◽  
Dna Fragmentation ◽  
Density Gradient ◽  
Embryo Development ◽  
Sperm Quality ◽  
Early Embryo ◽  
Control Group ◽  
Sperm Selection ◽  
Foetal Development ◽  
Development Rates

The objective of this work was to elucidate whether a sperm selection method that combines rheotaxis and microfluidics can improve the selection of spermatozoa over density gradient and swim-up. For this purpose human sperm selected by rheotaxis were compared against density gradient, swim-up and a control group of non-selected spermatozoa in split frozen-thawed (FT) and fresh (F) semen samples. Sperm quality was assessed in terms of motility, morphology, DNA fragmentation index (DFI), viability, acrosome integrity and membrane fluidity. Using a mouse model, we compared fertilisation and embryo development rates after performing ICSI with spermatozoa, sorted using rheotaxis or swim-up. Selection by rheotaxis yielded a sperm population with reduced DFI than the control (P < 0.05), improved normal morphology (P < 0.001) and higher total motility (TM; P < 0.001) than the other techniques studied in F and FT samples. Swim-up increased TM compared to density gradient and control in FT or F samples (P < 0.001), and yielded lower DFI than the control with F samples (P < 0.05). In FT samples, selection by rheotaxis yielded sperm with higher viability than control, density gradient and swim-up (P < 0.01) while acrosomal integrity and membrane fluidity were maintained. When mouse spermatozoa were selected for ICSI using rheotaxis compared to swim-up, there was an increase in fertilisation (P < 0.01), implantation (P < 0.001) and foetal development rates (P < 0.05). These results suggest that, in the absence of non-destructive DNA testing, the positive rheotaxis can be used to select a population of low DNA fragmentation spermatozoa with high motility, morphology and viability, leading to improved embryo developmental rates.

P–096 Real-time ranking of single spermatozoa using artificial vision analysis of complex motility patterns during ICSI aimed at improving fertilization and blastocyst development

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
A Chave Badiola ◽  
G Mendizabal ◽  
J Cohen ◽  
A Flores-Saiffe ◽  
V M Roberto ◽  
...  
Keyword(s):  
Real Time ◽  
Embryo Development ◽  
Selection Process ◽  
Movement Patterns ◽  
Artificial Vision ◽  
P Value ◽  
Sperm Selection ◽  
Blastocyst Formation ◽  
Rotational Movement ◽  
Normal Fertilization

Abstract Study question Can real-time artificial vision identify beneficial movement patterns of single spermatozoa in a cohort visualized in PVP during ICSI possibly enhancing fertilization and embryo development? Summary answer Artificial vision seems able to identify advantageous movement patterns of individual spermatozoa having a significant impact on both normal fertilization and blastocyst formation. What is known already Spermatozoa isolated from poor semen may reduce the quality of embryo development and blastocyst formation. Normal motility is dependent on general sperm morphology and characteristic movement of the flagellum enabling forward mobility. Spermatozoa roll as they swim. It is known that this rotational motion around their longitudinal axis promotes rheotaxis, which is a mechanism that allows the sperm to navigate to the site of fertilization. Therefore, it is possible that the characteristics of the rotational movement are related to sperm quality. Study design, size, duration Non-intervention study based on a cohort of 132 videos of in-vitro fertilization treatments with ICSI during which the sperm selection process was recorded up to sperm injection. The study was performed at one IVF center within a 6-month period. Injected spermatozoa and their corresponding oocytes were individually assessed from fertilization to blastocyst formation. Videos, where spermatozoa selected for injection could not be identified, were excluded. Relevant outcomes included normal fertilization (2PN), and blastocyst formation. Participants/materials, setting, methods Using a digitizer attached to an optical microscope (640 x 480 pixels), videos were recorded to include the sperm selection process, immobilization, and subsequent injection following standard ICSI protocols. Individual spermatozoa motility features were extracted using a proprietary computer-vision algorithm (SID, IVF 2.0 LTD). The rotational movements of spermatozoa were inferred by computing the variations of the mean intensity of the sperm in the video-sequence across time (MI). Main results and the role of chance Based on SID’s analysis, we found statistically significant differences between the median prominences of the MI of those injected spermatozoa that resulted in successful fertilization in comparison to those with failed fertilization (p-value=0.029, 28 negative fertilization, and 71 positive fertilization) using a one-tailed t-Student test with a significance level of 5%. We also found statistically significant differences between the median prominences of the MI of those spermatozoa that resulted in blastocysts in comparison with the spermatozoa-oocyte cohorts which didn’t reach the blastocyst stage (p-value 0.004, 51 with negative blastocyst formation and 48 with blastocyst formation). Limitations, reasons for caution The size of this database is modest, therefore a larger study with multiple clinics will be necessary to confirm the findings. Large prominence does not necessarily assurance successful fertilization or blastocyst formation since there may be other factors such as oocyte quality or the ICSI technique. Wider implications of the findings: Objective assessment of sperm rotational movement is difficult to quantify and to be objectively assessed during standard sperm selection. Real-time artificial vision tools such as SID could assist embryologists during the sperm selection process for ICSI. Trial registration number NA

Sperm selection based on motility in polyvinylpyrrolidone is associated with successful pregnancy and embryo development

Andrologia ◽  
2012 ◽  
Vol 45 (4) ◽  
pp. 240-247 ◽  
Author(s):  
T. Irez ◽  
P. Ocal ◽  
O. Guralp ◽  
S. Kaleli ◽  
F. Ocer ◽  
...  
Keyword(s):  
Embryo Development ◽  
Sperm Selection ◽  
Successful Pregnancy

scholarly journals Successful Pregnancy in a Couple with Severe Male Factor Infertility after Selection of Sperm with Cytoplasmic Droplets

2015 ◽  
Vol 2015 ◽  
pp. 1-4 ◽  
Author(s):  
Jenna Bellish ◽  
David H. McCulloh ◽  
Khaliq Ahmad ◽  
Peter G. McGovern
Keyword(s):  
Dna Fragmentation ◽  
Embryo Development ◽  
Male Partner ◽  
Male Factor Infertility ◽  
Sperm Selection ◽  
Selection Methods ◽  
Male Factor ◽  
Successful Pregnancy ◽  
Live Births ◽  
Selection Of

We present live births resulting from two separate IVF cycles in a couple in which ICSI was performed with sperm specifically selected for presence of small cytoplasmic droplets. These cycles followed previous cycles using standard sperm selection methods in which very poor embryo development and no pregnancies ensued. The male partner was diagnosed with severe male factor infertility including elevated DNA fragmentation.

Effect of oocyte maturation time, sperm selection method and oxygen tension on in vitro embryo development in alpacas

2017 ◽  
Vol 95 ◽  
pp. 127-132 ◽  
Author(s):  
Jaime Ruiz ◽  
R. Paulo Santayana ◽  
M. José Mendoza ◽  
J. Leandra Landeo ◽  
Elizabeth Huamán ◽  
...  
Keyword(s):  
Oxygen Tension ◽  
Embryo Development ◽  
Oocyte Maturation ◽  
Selection Method ◽  
Sperm Selection ◽  
Maturation Time

68 Comparison of two Percoll gradients for selection of frozen semen for invitro production of ovine embryos

2021 ◽  
Vol 33 (2) ◽  
pp. 141
Author(s):  
A. Velázquez-Roque ◽  
F. Villaseñor-González ◽  
G. Márquez-Márquez ◽  
M. Kjelland ◽  
H. Álvarez-Gallardo ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Physiological Saline ◽  
Bottom Layer ◽  
Penicillin G ◽  
Sperm Selection ◽  
Percoll Gradient ◽  
Cleavage Rate ◽  
Frozen Semen ◽  
Percoll Gradients ◽  
Α Level

Sperm selection methods are routinely applied to prepare semen for IVF in animal species. These procedures are used to improve sperm quality characteristics as well as to remove other background material and debris. Percoll gradient is widely used in animal IVF laboratories. Different Percoll gradient concentrations and volumes can be used to improve the sperm sample motility percentage. The objective of this research was to evaluate the effect of 2 different Percoll concentrations for ovine IVF sperm selection and effects, if any, on invitro embryo production (IVP). Specifically, Percoll gradients consisted of Group 1 (G1) 40–80% and Group 2 (G2) 45–90%, 400µL each. The research was carried out in the reproduction laboratory at Palominos Ranch (Jalisco, México). The IVP was performed with a continuous invitro culture system. Ovaries (n=157) were collected from a slaughterhouse (León, México) and transported to the laboratory within 2h in physiological saline solution (0.9% NaCl) supplemented with penicillin G (100IU mL−1) and streptomycin sulphate (100µg mL−1). For IVP, IVF Bioscience™ media were used for IVM, IVF, and invitro culture (IVC). For IVM, the cumulus–oocyte complexes (COCs) were selected (only grades 1 and 2) and matured for 24h at 38.5°C in 5% CO2 in air and 100% humidity. Matured oocytes (n=800, divided equally into 4 replicates) were divided into 2 groups, G1 and G2. The IVF process was conducted with semen selected through a mini-Percoll technique with gradients at a concentration of 45% (top layer) and 90% (bottom layer) or 40% (top layer) and 80% (bottom layer) for G1 and G2, respectively, using frozen–thawed semen from the same ram, at a concentration of 2×106 sperm mL−1, for 18h in 38.5°C, 5% CO2 in air, and 100% humidity. The presumptive zygotes were denuded by pipetting and set in IVC until Day 7 at 38.5°C, 5% CO2, 5% O2, and 90% N2 at 100% humidity. The percentages of cleavage, embryos with more than 6 cells, and blastocysts on Day 7 of culture were evaluated, based on the initial number of oocytes entering into IVM. The statistical analyses were carried out with the GLM procedure of SAS software (version 9.3; SAS Institute Inc.) to evaluate the results of G1 versus G2 (α level=0.05). Cleavage rate was 47.8%±2.5 and 55.9%±2.5, respectively, in G1 and G2. The percentages of embryos with more than 6 cells were 38.1%±2.2 and 43.5%±2.2, respectively, in G1 and G2. The percentage of blastocysts on Day 7 was 27.4%±1.1 and 37.3%±1.1, respectively, in G1 and G2. There were no significant differences between groups (P&gt;0.05) for percentage of cleavage and embryos with more than 6 cells, although the percentage of cleavage tended to be greater for G2 (P=0.06). Additionally, G2 had a larger percentage of blastocysts on Day 7 compared with G1 (P&lt;0.05). In conclusion, under the conditions of this research, the use of a Percoll gradient at a concentration of 40–80% increased the percentage of blastocysts for ovine IVP.

scholarly journals Effect the sperm selection magnification (400x vs 1,200x) on fertilization results and embryo development in human Piezo-ICSI

2017 ◽  
Vol 108 (3) ◽  
pp. e147
Author(s):  
K.K. Hiraoka ◽  
Y. Otsuka ◽  
T. Ishikawa ◽  
K. Kawai ◽  
T. Harada
Keyword(s):  
Embryo Development ◽  
Sperm Selection
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