scholarly journals Euthanasia via CO 2 inhalation causes premature cortical granule exocytosis in mouse oocytes and influences in vitro fertilization and embryo development

2019 ◽  
Vol 86 (7) ◽  
pp. 825-834 ◽  
Author(s):  
Liga Wuri ◽  
Cansu Agca ◽  
Yuksel Agca
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Cortical Granule ◽  
Granule Exocytosis ◽  
Vitro Fertilization ◽  
Mouse Oocytes ◽  
Cortical Granule Exocytosis

scholarly journals Live imaging of cortical granule exocytosis reveals that in vitro matured mouse oocytes are not fully competent to secrete their content

Biology Open ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. bio031872 ◽  
Author(s):  
Andrea I. Cappa ◽  
Matilde de Paola ◽  
Paula Wetten ◽  
Gerardo A. De Blas ◽  
Marcela A. Michaut
Keyword(s):  
Live Imaging ◽  
Cortical Granule ◽  
Granule Exocytosis ◽  
Mouse Oocytes ◽  
Cortical Granule Exocytosis

scholarly journals Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0135679 ◽  
Author(s):  
Matilde de Paola ◽  
Oscar Daniel Bello ◽  
Marcela Alejandra Michaut
Keyword(s):  
Cortical Granule ◽  
Granule Exocytosis ◽  
Mouse Oocytes ◽  
Sensitive Factor ◽  
Cortical Granule Exocytosis

scholarly journals Mouse oocytes derived from fetal germ cells are competent to support the development of embryos by in vitro fertilization

2008 ◽  
Vol 75 (11) ◽  
pp. 1688-1689
Author(s):  
Wei Shen ◽  
Lan Li ◽  
Donghui Zhang ◽  
Qingjie Pan ◽  
Mingxiao Ding ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Germ Cells ◽  
Vitro Fertilization ◽  
Mouse Oocytes

Predictive variables of in vitro fertilization and pre-implantation embryo development in the mouse

2002 ◽  
Vol 63 (1) ◽  
pp. 38-46 ◽  
Author(s):  
Juan J. TarÍn ◽  
Vanessa Gómez-Piquer ◽  
Sonia PÉrez-AlbalÁ ◽  
Carlos Hermenegildo ◽  
Antonio Cano
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Vitro Fertilization ◽  
Predictive Variables

scholarly journals Phosphoprotein inhibition of calcium-stimulated exocytosis in sea urchin eggs.

1991 ◽  
Vol 113 (4) ◽  
pp. 769-778 ◽  
Author(s):  
T Whalley ◽  
I Crossley ◽  
M Whitaker
Keyword(s):  
Sea Urchin ◽  
Okadaic Acid ◽  
Cortical Granule ◽  
Granule Exocytosis ◽  
Sea Urchin Eggs ◽  
Transduction Mechanisms ◽  
Egg Signal ◽  
Cortical Granule Exocytosis ◽  
Gamma S

We have investigated the role of protein phosphorylation in the control of exocytosis in sea urchin eggs by treating eggs with a thio-analogue of ATP. ATP gamma S (adenosine 5'-O-3-thiotriphosphate) is a compound which can be used as a phosphoryl donor by protein kinases, leading to irreversible protein thiophosphorylation (Gratecos, D., and E.H. Fischer. 1974. Biochem. Biophys. Res. Commun. 58:960-967). Microinjection of ATP gamma S inhibits cortical granule exocytosis, but has no effect on the sperm-egg signal transduction mechanisms which normally cause exocytosis by generating an increase in [Ca2+]i. ATP gamma S requires cytosolic factors for its inhibition of cortical granule exocytosis: it does not affect exocytosis when applied directly to the isolated exocytotic apparatus. Our data suggest that ATP gamma S irreversibly inhibits exocytosis via thiophosphorylation of proteins associated with the egg cortex. We have identified two thiophosphorylated proteins (33 and 27 kD) that are associated with the isolated exocytotic apparatus. They may mediate the inhibition of exocytosis by ATP gamma S. In addition, we show that okadaic acid, an inhibitor of phosphoprotein phosphatases, prevents cortical granule exocytosis at fertilization without affecting calcium mobilization. Like ATP gamma S, okadaic acid has no effect on exocytosis in vitro. Our results suggest that an inhibitory phosphoprotein can obstruct calcium-stimulated exocytosis in sea urchin eggs; on the other hand, they do not readily support the idea that a protein phosphatase is an essential component of the mechanism controlling exocytosis.

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