Modulation of complex coordinated molecular signaling by 5HT and a cocktail of inhibitors leads to ovarian maturation of Penaeus monodon in captivity

2019 ◽  
Vol 86 (5) ◽  
pp. 576-591
Author(s):  
Thiyagu Mani ◽  
Bharathi Raja Subramaniya ◽  
Sowmya Chidambaram Iyer ◽  
Niranjali Devaraj Sivasithamparam ◽  
Halagowder Devaraj
Keyword(s):  
Penaeus Monodon ◽  
Molecular Signaling ◽  
Ovarian Maturation ◽  
In Captivity

Dynamics of fatty acid regulatory genes during ovarian development in Penaeus monodon

Reproduction ◽  
2018 ◽  
Author(s):  
Pacharawan Deenarn ◽  
Punsa Tobwor ◽  
Rungnapa Leelatanawit ◽  
Somjai Wongtriphop ◽  
Jutatip Khudet ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Ovarian Development ◽  
Penaeus Monodon ◽  
Chain Fatty Acid ◽  
Regulatory Genes ◽  
Ovarian Maturation ◽  
Long Chain Fatty Acid ◽  
Eyestalk Ablation ◽  
Regulatory Pathways ◽  
Expression Levels

The delay in ovarian maturation in farmed black tiger shrimp Penaeus monodon has resulted in the widespread practice of feeding broodstock with the polychaetes Perinereis nuntia and their unilateral eyestalk ablation. Although this practice alters fatty acid content in shrimp ovaries and hepatopancreas, its effects on fatty acid regulatory genes have yet to be systematically examined. Here, microarray analysis was performed on hepatopancreas and ovary cDNA collected from P. monodon at different ovarian maturation stages, revealing that 72 and 58 genes in fatty acid regulatory pathways were differentially expressed in hepatopancreas and ovaries respectively. Quantitative real-time PCR analysis revealed that ovarian maturation was associated with higher expression levels of acetyl-CoA acetyltransferase, acyl-CoA dehydrogenase, acyl-CoA oxidase 3 and long-chain fatty acid transport protein 4 in hepatopancreas, whereas the expression levels of 15 fatty acid regulatory genes were increased in shrimp ovaries. To distinguish the effects of different treatments, transcriptional changes were examined in P. monodon with stage 1 ovaries before polychaete feeding, after one-month of polychaete feeding and after eyestalk ablation. Polychaete feeding resulted in lower expression levels of enoyl-CoA hydratase and acyl-CoA synthetase medium-chain family member 4, while the expression level of phosphatidylinositide phosphatase SAC1 was higher in shrimp hepatopancreas and ovaries. Additionally, eyestalk ablation resulted in a higher expression level of long-chain fatty acid-CoA ligase 4 in both tissues. Together, our findings describe the dynamics of fatty acid regulatory pathways during crustacean ovarian development and provide potential target genes for alternatives to eyestalk ablation in the future.

The existence of gonadotropin-releasing hormone (GnRH) immunoreactivity in the ovary and the effects of GnRHs on the ovarian maturation in the black tiger shrimp Penaeus monodon

Aquaculture ◽  
2008 ◽  
Vol 279 (1-4) ◽  
pp. 197-203 ◽  
Author(s):  
Apichart Ngernsoungnern ◽  
Piyada Ngernsoungnern ◽  
Wattana Weerachatyanukul ◽  
Jittipan Chavadej ◽  
Prasert Sobhon ◽  
...  
Keyword(s):  
Penaeus Monodon ◽  
Gonadotropin Releasing Hormone ◽  
Ovarian Maturation ◽  
Releasing Hormone ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp

Serotonin stimulates ovarian maturation and spawning in the black tiger shrimp Penaeus monodon

Aquaculture ◽  
2006 ◽  
Vol 261 (4) ◽  
pp. 1447-1454 ◽  
Author(s):  
Kanokpan Wongprasert ◽  
Somluk Asuvapongpatana ◽  
Pisit Poltana ◽  
Montip Tiensuwan ◽  
Boonsirm Withyachumnarnkul
Keyword(s):  
Penaeus Monodon ◽  
Ovarian Maturation ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp

scholarly journals Gonadal maturation, fecundity and hatching performance of wild caught tiger shrimp Penaeus monodon using unilateral eyestalk ablation in captivity

2016 ◽  
Vol 13 (2) ◽  
pp. 315-322 ◽  
Author(s):  
SA Uddin ◽  
MM Rahman
Keyword(s):  
Significant Positive Correlation ◽  
Penaeus Monodon ◽  
Hatching Rate ◽  
Environmental Condition ◽  
Eyestalk Ablation ◽  
Multiple Spawning ◽  
In Captivity ◽  
Investigation Period ◽  
Moulting Cycle ◽  
Average Fertility

In this study, the effects of eyestalk ablation on maturation, moulting, spawning and hatching rate of wild caught Penaeus monodon were investigated. Twenty males (M) and 30 eyestalk–ablated and 30 non–ablated females (F), all individually marked (M: F=1:3) were stocked into two 15–ton capacity (each) maturation tank. All the ablated females moulted in 7.92±0.24 (mean±standard error) days, and first spawning occurred 3.2±0.20 days following eyestalk ablation. While twenty-five ablated females spawned, producing an average of 296,160±26,589 eggs, only eight non–ablated females spawned, producing an average of 195,462±20,565 eggs during the investigation period of 120 days. A significant positive correlation between fecundity and female body weight (P<0.05; P<0.001) was observed. Throughout the experiment, the average number of spawning per female was 1.8±0.14 for ablated and one for non–ablated females. Multiple spawning (up to three times) occurred within the same moulting cycle in ablated females. Among the ablated P. monodon females, 36% spawn once, 48% second time, and 16% third time. The average fertility rate of the eggs was high, ranging between 80 and 90% in non-ablated females, while in ablated females; the range was between 72 and 88% and differ significantly (p<0.05) ablated and non ablated females. Average hatching rate ranges between 70 and 80% for ablated and 75 and 82% for non-ablated females (p<0.05). From eggs to nauplii production per female was 167,838 for ablated and 127,500for non–ablated females. This study indicates that eyestalk ablation and environmental condition were important inducing tools for re-maturation of spent P. monodon that continued supply of seed stock for effective commercial shrimp farming.J. Bangladesh Agril. Univ. 13(2): 315-322, December 2015

scholarly journals Comparison of ovarian maturation and spawning after unilateral eyestalk ablation of wild-caught and pond-reared Penaeus monodon

2015 ◽  
Vol 13 (3) ◽  
pp. e0402 ◽  
Author(s):  
Weigeng Wen ◽  
Qibin Yang ◽  
Zhenhua Ma ◽  
Shigui Jiang ◽  
Lihua Qiu ◽  
...  
Keyword(s):  
Body Weight ◽  
Egg Production ◽  
Penaeus Monodon ◽  
Size Group ◽  
Hatching Rate ◽  
Weight Class ◽  
Ovarian Maturation ◽  
Egg Hatching ◽  
Eyestalk Ablation ◽  
Spawning Time

<p>The present study compares the efficiency of ovarian maturation and spawning success between wild-caught and pond-reared <em>Penaeus monodon </em>females after unilateral eyestalk ablation. The earliest spawning time after eyestalk ablation was 5.9 days in wild-caught females, which is significantly shorter than the spawning time in pond-reared females (10.5 days). Both wild-caught and pond-reared females repeatedly spawned after eyestalk ablation. On average, each wild-caught female spawned 2.94 times while each pond-reared female spawned only 1.09 times. The spawning induction rate, egg hatching rate, and the number of eggs per spawning were significantly greater in wild-caught females than in pond-reared females. However, the egg size was not significantly different between wild-caught and pond-reared females. Four shrimp sizes (60, 80, 100 and 120 (± 1.0) g) were tested in this study and body weight significantly affected ovarian induction in pond-reared females but not in wild-caught females. Within the same body-weight class, the egg number per spawn in wild-caught females was significantly greater than that in pond-reared females. The egg production per spawn of the pond-reared females in the 120-g size group was two times higher than that in the pond-reared females in the 80-g size group. In conclusion, the fecundity of wild-caught <em>P. monodon</em> females is significantly higher than that of pond-reared <em>P. monodon</em> females. In breeding pond-reared <em>P. monodon</em>, the recommended minimum body weight of females is over 80 g, and the desirable body weight is over 100 g.</p>

scholarly journals Changes in oviduct structure in the black tiger shrimp, Penaeus monodon, during ovarian maturation

2012 ◽  
Vol 13 (10) ◽  
pp. 846-850 ◽  
Author(s):  
Sitthichai Iamsaard ◽  
Siriporn Sriurairatana ◽  
Boonsirm Withyachumnarnkul
Keyword(s):  
Penaeus Monodon ◽  
Ovarian Maturation ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp
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