Noncoding RNAs: Potential players in the self-renewal of mammalian spermatogonial stem cells

2018 ◽  
Vol 85 (8-9) ◽  
pp. 720-728 ◽  
Author(s):  
Beibei Bie ◽  
Ya Wang ◽  
Liang Li ◽  
Huanle Fang ◽  
Libing Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Noncoding Rnas ◽  
Spermatogonial Stem Cells ◽  
The Self ◽  
Self Renewal

Both Gfrα1a and Gfrα1b Are Involved in the Self-Renewal and Maintenance of Spermatogonial Stem Cells in Medaka

2018 ◽  
Vol 27 (23) ◽  
pp. 1658-1670
Author(s):  
Yang Zhao ◽  
Zhuo Yang ◽  
Yuan Wang ◽  
Yubing Luo ◽  
Fan Da ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
The Self ◽  
Self Renewal

scholarly journals MicroRNA-21 regulates the self-renewal of mouse spermatogonial stem cells

2011 ◽  
Vol 108 (31) ◽  
pp. 12740-12745 ◽  
Author(s):  
Z. Niu ◽  
S. M. Goodyear ◽  
S. Rao ◽  
X. Wu ◽  
J. W. Tobias ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
The Self ◽  
Self Renewal

scholarly journals Mouse thy1-positive spermatogonia suppress the proliferation of spermatogonial stem cells by Extracellular vesicles in vitro

2020 ◽  
Author(s):  
Yu Lin ◽  
Qian Fang ◽  
Yue He ◽  
Xiaowen Gong ◽  
Yinjuan Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Vesicles ◽  
Magnetic Beads ◽  
Spermatogonial Stem Cells ◽  
The Self ◽  
Seminiferous Tubules ◽  
Self Renewal

ABSTRACTThe self-renewal of mammalian spermatogonial stem cells (SSCs) supports spermatogenesis to produce spermatozoa, and this is precisely controlled in a stem niche microenvironment in the seminiferous tubules. Although studies have revealed the role of the surrounding factors in SSCs, little is known about whether the division of SSCs is controlled by extracellular vesicles. Here, extracellular vesicles were found in the basal compartment of seminiferous tubules in mouse, rat, rabbit and human testes. In the mice, the testicular extracellular vesicles are secreted by spermatogonia and are taken up by SSCs. Further, the extracellular vesicles from thy1-positive spermatogonia were purified by anti-Thy1-coupled magnetic beads, and which suppress their proliferation of SSCs but not lead to the apoptosis in vitro.

Thy1-positive spermatogonia suppress the proliferation of spermatogonial stem cells by Extracellular vesicles in vitro

Endocrinology ◽  
2021 ◽  
Author(s):  
Lin Yu ◽  
Qian Fang ◽  
Yue He ◽  
Xiaowen Gong ◽  
Yinjuan Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Vesicles ◽  
Magnetic Beads ◽  
Spermatogonial Stem Cells ◽  
The Self ◽  
Seminiferous Tubules ◽  
Self Renewal

Abstract The self-renewal of mammalian spermatogonial stem cells (SSCs) supports spermatogenesis to produce spermatozoa, and this is precisely controlled in a stem niche microenvironment in the seminiferous tubules. Although studies have revealed the role of the surrounding factors in SSCs, little is known about whether the division of SSCs is controlled by extracellular vesicles. Here, extracellular vesicles were found in the basal compartment of seminiferous tubules in mouse, rat, rabbit and human testes. In the mice, the testicular extracellular vesicles are secreted by spermatogonia and are taken up by SSCs. Further, the extracellular vesicles from thy1-positive spermatogonia were purified by anti-Thy1-coupled magnetic beads, and which suppress their proliferation of SSCs but not lead to the apoptosis in vitro.

scholarly journals Hsa-miR-1908-3p Mediates the Self-Renewal and Apoptosis of Human Spermatogonial Stem Cells via Targeting KLF2

2020 ◽  
Vol 20 ◽  
pp. 788-800
Author(s):  
Wei Chen ◽  
Yinghong Cui ◽  
Bang Liu ◽  
Chunyun Li ◽  
Li Du ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
The Self ◽  
Self Renewal

scholarly journals Chemokine (C-X-C) Ligand 12 Facilitates Trafficking of Donor Spermatogonial Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Zhiyv Niu ◽  
Shaun M. Goodyear ◽  
Mary R. Avarbock ◽  
Ralph L. Brinster
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Primordial Germ Cells ◽  
Critical Role ◽  
Spermatogonial Stem Cells ◽  
The Self ◽  
Self Renewal ◽  
Critical Window ◽  
Cxcr4 Signaling

The chemokine (C-X-C) receptor type 4 (CXCR4) is an early marker of primordial germ cells (PGCs) essential for their migration and colonization of the gonads. In spermatogonial stem cells (SSCs), the expression of CXCR4 is promoted by the self-renewal factor, glial cell line-derived neurotrophic factor (GDNF). Here, we demonstrate an important role of CXCR4 during donor mouse SSCs reoccupation of the endogenous niche in recipient testis. Silencing of CXCR4 expression in mouse SSCs dramatically reduced the number of donor stem cell-derived colonies, whereas colony morphology and spermatogenesis were comparable to controls. Inhibition of CXCR4 signaling using a small molecule inhibitor (AMD3100) during the critical window of homing also significantly lowered the efficiency of donor-derived SSCs to establish spermatogenic colonies in recipient mice; however, the self-renewal of SSCs was not affected by exposure to AMD3100. Rather,in vitromigration assays demonstrate the influence of CXCR4-CXCL12 signaling in promoting germ cell migration. Together, these studies suggest that CXCR4-CXCL12 signaling functions to promote homing of SSCs towards the stem cell niche and plays a critical role in reestablishing spermatogenesis.

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