The effects of mitochondrial DNA supplementation at the time of fertilization on the gene expression profiles of porcine preimplantation embryos

2018 ◽  
Author(s):  
Te-Sha Tsai ◽  
Justin C. St. John
Keyword(s):  
Gene Expression ◽  
Mitochondrial Dna ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Preimplantation Embryos

scholarly journals Differences in gene expression profiles between human preimplantation embryos cultured in two different IVF culture media

2015 ◽  
Vol 30 (10) ◽  
pp. 2303-2311 ◽  
Author(s):  
Sander H.M. Kleijkers ◽  
Lars M.T. Eijssen ◽  
Edith Coonen ◽  
Josien G. Derhaag ◽  
Eleni Mantikou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Culture Media ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Preimplantation Embryos ◽  
Human Preimplantation Embryos

Single-cell RNA sequencing reveals distinct gene expression patterns in glucose metabolism of human preimplantation embryos

2019 ◽  
Vol 31 (2) ◽  
pp. 237 ◽  
Author(s):  
Di-Cheng Zhao ◽  
Yu-Mei Li ◽  
Jie-Liang Ma ◽  
Ning Yi ◽  
Zhong-Yuan Yao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Glucose Metabolism ◽  
Single Cell ◽  
Glucose Transporter ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Gene Expression Profiles ◽  
Preimplantation Embryos ◽  
Morula Stage ◽  
Human Preimplantation Embryos

Precise regulation of glucose metabolism-related genes is essential for early embryonic development. Although previous research has yielded detailed information on the biochemical processes, little is yet known of the dynamic gene expression profiles in glucose metabolism of preimplantation embryos at a single-cell resolution. In the present study, we performed integrated analysis of single-cell RNA sequencing (scRNA-seq) data of human preimplantation embryos that had been cultured in sequential medium. Different cells in the same embryo have similar gene expression patterns in glucose metabolism. During the switch from the cleavage to morula stage, the expression of glycolysis-related genes, such as glucose transporter genes (solute carrier family 2 (facilitated glucose transporter), member 1 (SLC2A1) and solute carrier family 2 (facilitated glucose transporter), member 3 (SLC2A3) and genes encoding hexokinase, phosphofructokinase, pyruvate kinase and lactate dehydrogenase, is increased. The genes involved in the pentose phosphate pathway are highly expressed at the cleavage stage, generating the reducing power to balance oxidative stress derived from biosynthesis. Expression of the genes involved in the biosynthesis of glycerophospholipids is increased after the morula stage. Nevertheless, the expression of tricarboxylic acid-related genes remains relatively unchanged during the preimplantation stages. In conclusion, we discovered that the gene expression profiles are dynamic according to glucose utilisation in the embryos at different stages, which contributes to our understanding of regulatory mechanisms of glucose metabolism-related genes in human preimplantation embryos.

scholarly journals P–242 Gene expression profiles of SARS-CoV–2-associated receptors and proteases in human early embryonic development and follicular cells

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
F Entezami ◽  
D Haouzi ◽  
S Brouillet ◽  
F Barry ◽  
A Gala ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Profiles ◽  
Female Genital Tract ◽  
Gene Expression Profiles ◽  
Cumulus Cells ◽  
High Expression ◽  
Expression Level ◽  
Preimplantation Embryos ◽  
Day 3 Embryos ◽  
High Expression Level

Abstract Study question Are the oocytes, embryos, granulosa and cumulus cells, used during ART, susceptible to the SARS-CoV–2 infection? Summary answer Transcriptomic analyses of SARS-CoV–2-associated receptors and proteases strongly suggest that blastocysts are most permissive to SARS-CoV–2 compared with mature oocytes and day 3 embryos. What is known already Very few studies analyzed the gene expression profiles of SARS-CoV–2-associated receptors and proteases, mainly focusing on ACE2 and TMPRSS2 expression, resulting in partial knowledge in different specimens from female genital tract. To date, the gene expression profile of SARS-CoV–2 host entry candidates in the entire preimplantation embryos is scarcely available. Moreover, reports on oocyte and granulosa cells susceptibilityto SARS-CoV–2 are very sparse. Study design, size, duration To address this question, we retrospectively examined the gene expression profiles of SARS-CoV–2-associated receptors and proteases in human granulosa cells (GCs), cumulus cells (CCs), mature oocytes, day 3 embryos, blastocysts and trophectoderm cells obtained from our previously described Affymetrix microarray data. Participants/materials, setting, methods Human GCs and CCs (n = 17), mature oocytes (n = 6), and preimplantation embryos (n = 20) were analyzed. The comparison of gene expression levels of receptors and proteases closely related to SARS-CoV–2 infection. For each gene, the number of samples with the probe set ‘present’, based on the detection call, was analyzed. Each probe set was classified according to the signal intensity value median, as low (<100), medium (100–200) or high expression level (>200). Main results and the role of chance ACE2, BSG, CTSL, CTSA were detectable at high expression level in all mature oocyte samples, while only CTSL was strongly expressed in all day 3 embryos. The most representative dual co-expression of SARS-CoV–2-associated receptor and protease (60% of samples) during the embryonic genome activation stage (EGA) was ACE2-CTSL and BSG-CTSL. In blastocysts, ACE2, BSG, CTSL, CTSA and FURIN were detectable in the entire cohort at high expression level, and the prevalence of the different dual co-expression of SARS-CoV–2-associated proteases and receptors was optimal (100% of samples). Interestingly, only CTSL was detectable in all trophectoderm samples and a prevalence of 60% was found for the BSG-CTSL co-expression. ACE2, BSG, CTSL and CTSA were present at high expression level in CCs samples. In contrast, ACE2 and BSG expression was very low while CTSL and CTSA showed a high expression level in GCs.A prevalence of 100% was reported for ACE2-CTSL, ACE2-CTSA co-expression for both cell types. In addition, BSG-CTSL and BSG-CTSA co-expression were also present in all CCs against ∼70% in GCs samples. This data suggest a potential risks of SARS-CoV–2 infection either GC or early embryo development. Limitations, reasons for caution Analyses of Affymetrix microarray gene expression data were performed in non-COVID–19 patients. Whether the SARS-CoV–2 infection change the gene expression profile of SARS-CoV–2-associated receptors and proteases is under investigation. Wider implications of the findings: Specimens from female genital tract may be considered as potential targets for SARS-CoV–2. Trial registration number Not applicable

1327: Gene Expression Profiles in Benign Prostatic Hyperplasia

2004 ◽  
Vol 171 (4S) ◽  
pp. 349-350
Author(s):  
Gaelle Fromont ◽  
Michel Vidaud ◽  
Alain Latil ◽  
Guy Vallancien ◽  
Pierre Validire ◽  
...  
Keyword(s):  
Gene Expression ◽  
Benign Prostatic Hyperplasia ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Prostatic Hyperplasia
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