scholarly journals Bovine trophectoderm cells induced from bovine fibroblasts with induced pluripotent stem cell reprogramming factors

2017 ◽  
Vol 84 (6) ◽  
pp. 468-485 ◽  
Author(s):  
Neil C. Talbot ◽  
Wendy O. Sparks ◽  
Caitlin E. Phillips ◽  
Alan D. Ealy ◽  
Anne M. Powell ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Cell Reprogramming ◽  
Trophectoderm Cells ◽  
Reprogramming Factors ◽  
Induced Pluripotent

scholarly journals cAMP and EPAC Signaling Functionally Replace OCT4 During Induced Pluripotent Stem Cell Reprogramming

2015 ◽  
Vol 23 (5) ◽  
pp. 952-963 ◽  
Author(s):  
Ashley L Fritz ◽  
Maroof M Adil ◽  
Sunnie R Mao ◽  
David V Schaffer
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Cell Reprogramming ◽  
Induced Pluripotent ◽  
Epac Signaling

scholarly journals Brief Report: Analysis of EndogenousOct4Activation during Induced Pluripotent Stem Cell Reprogramming Using an InducibleOct4Lineage Label

Stem Cells ◽  
2012 ◽  
Vol 30 (11) ◽  
pp. 2596-2601 ◽  
Author(s):  
Lucas V. Greder ◽  
Sandeep Gupta ◽  
Shunan Li ◽  
Md. Joynal Abedin ◽  
Abdulrahim Sajini ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Cell Reprogramming ◽  
Report Analysis ◽  
Induced Pluripotent

A PRC2-Dependent Repressive Role of PRDM14 in Human Embryonic Stem Cells and Induced Pluripotent Stem Cell Reprogramming

Stem Cells ◽  
2013 ◽  
Vol 31 (4) ◽  
pp. 682-692 ◽  
Author(s):  
Yun-Shen Chan ◽  
Jonathan Göke ◽  
Xinyi Lu ◽  
Nandini Venkatesan ◽  
Bo Feng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Pluripotent Stem Cell ◽  
Embryonic Stem ◽  
Induced Pluripotent Stem Cell ◽  
Cell Reprogramming ◽  
Induced Pluripotent

scholarly journals Baboon induced pluripotent stem cell generation by piggyBac transposition of reprogramming factors

2019 ◽  
Vol 6 (2) ◽  
pp. 75-86 ◽  
Author(s):  
Ignacio Rodriguez-Polo ◽  
Michael Stauske ◽  
Alexander Becker ◽  
Iris Bartels ◽  
Ralf Dressel ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Large Body ◽  
Evolutionary Relationship ◽  
Induced Pluripotent Stem Cell ◽  
Papio Anubis ◽  
Cell Replacement ◽  
Ipsc Generation ◽  
Reprogramming Factors ◽  
Induced Pluripotent

Abstract. Clinical application of regenerative therapies using embryonic or induced pluripotent stem cells is within reach. Progress made during recent years has encouraged researchers to address remaining open questions in order to finally translate experimental cell replacement therapies into application in patients. To achieve this, studies in translationally relevant animal models are required to make the final step to the clinic. In this context, the baboon (Papio anubis) may represent a valuable nonhuman primate (NHP) model to test cell replacement therapies because of its close evolutionary relationship to humans and its large body size. In this study, we describe the reprogramming of adult baboon skin fibroblasts using the piggyBac transposon system. Via transposon-mediated overexpression of six reprogramming factors, we generated five baboon induced pluripotent stem cell (iPSC) lines. The iPSC lines were characterized with respect to alkaline phosphatase activity, pluripotency factor expression analysis, teratoma formation potential, and karyotype. Furthermore, after initial cocultivation with mouse embryonic fibroblasts, we were able to adapt iPSC lines to feeder-free conditions. In conclusion, we established a robust and efficient protocol for iPSC generation from adult baboon fibroblasts.

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