Regucalcin is widely distributed in the male reproductive tract and exerts a suppressive effect on in vitro sperm capacitation in the water buffalo (Bubalus bubalis )

2017 ◽  
Vol 84 (3) ◽  
pp. 212-221 ◽  
Author(s):  
Harikrishna Pillai ◽  
Ashish Mukunda Shende ◽  
Mehtab Sing Parmar ◽  
Anjaneya A ◽  
Sreela L ◽  
...  
Keyword(s):  
Reproductive Tract ◽  
Water Buffalo ◽  
Bubalus Bubalis ◽  
Suppressive Effect ◽  
Sperm Capacitation ◽  
Male Reproductive Tract

Post-warming hatching and birth of live calves following transfer of in vitro-derived vitrified water buffalo (Bubalus bubalis) embryos

2004 ◽  
Vol 61 (7-8) ◽  
pp. 1429-1439 ◽  
Author(s):  
Danilda Hufana-Duran ◽  
Prudencio B Pedro ◽  
Hernando V Venturina ◽  
Rogelio D Hufana ◽  
Apolinario L Salazar ◽  
...  
Keyword(s):  
Water Buffalo ◽  
Bubalus Bubalis

scholarly journals Effect of bull on in vitro sperm capacitation induced by different agents in buffalo species (Bubalus bubalis)

2009 ◽  
Vol 8 (sup2) ◽  
pp. 177-179 ◽  
Author(s):  
Luigi Zicarelli ◽  
Evelina Mariotti ◽  
Serena Di Francesco ◽  
Salvatore Velotto ◽  
Marcello Rubessa ◽  
...  
Keyword(s):  
Bubalus Bubalis ◽  
Sperm Capacitation

Causes of azoospermia and their management

2004 ◽  
Vol 16 (5) ◽  
pp. 561 ◽  
Author(s):  
P. N. Schlegel
Keyword(s):  
Reproductive Tract ◽  
Microscopic Analysis ◽  
In Vitro Fertilisation ◽  
Obstructive Azoospermia ◽  
Success Rates ◽  
Sperm Production ◽  
Sperm Retrieval ◽  
Prognostic Information ◽  
Male Reproductive Tract

Azoospermia may occur because of reproductive tract obstruction (obstructive azoospermia) or inadequate production of spermatozoa, such that spermatozoa do not appear in the ejaculate (non-obstructive azoospermia). Azoospermia is diagnosed based on the absence of spermatozoa after centrifugation of complete semen specimens using microscopic analysis. History and physical examination and hormonal analysis (FSH, testosterone) are undertaken to define the cause of azoospermia. Together, these factors provide a >90% prediction of the type of azoospermia (obstructive v. non-obstructive). Full definition of the type of azoospermia is provided based on diagnostic testicular biopsy. Obstructive azoospermia may be congenital (congenital absence of the vas deferens, idiopathic epididymal obstruction) or acquired (from infections, vasectomy, or other iatrogenic injuries to the male reproductive tract). Couples in whom the man has congenital reproductive tract obstruction should have cystic fibrosis (CF) gene mutation analysis for the female partner because of the high risk of the male being a CF carrier. Patients with acquired obstruction of the male reproductive tract may be treated using microsurgical reconstruction or transurethral resection of the ejaculatory ducts, depending on the level of obstruction. Alternatively, sperm retrieval with assisted reproduction may be used to effect pregnancies, with success rates of 25–65% reported by different centres. Non-obstructive azoospermia may be treated by defining the cause of low sperm production and initiating treatment. Genetic evaluation with Y-chromosome microdeletion analysis and karyotype testing provides prognostic information in these men. For men who have had any factors potentially affecting sperm production treated and remain azoospermic, sperm retrieval from the testis may be effective in 30–70% of cases. Once sperm are found, pregnancy rates of 20–50% may be obtained at different centres with in vitro fertilisation and intracytoplasmic sperm injection.

scholarly journals In vivo exposure to 17β-estradiol triggers premature sperm capacitation in cauda epididymis

Reproduction ◽  
2013 ◽  
Vol 145 (3) ◽  
pp. 255-263 ◽  
Author(s):  
Lukas Ded ◽  
Natasa Sebkova ◽  
Martina Cerna ◽  
Fatima Elzeinova ◽  
Pavla Dostalova ◽  
...  
Keyword(s):  
Reproductive Tract ◽  
Negative Impact ◽  
Reproductive Potential ◽  
Female Reproductive Tract ◽  
Sperm Capacitation ◽  
Epididymal Sperm ◽  
Knock Out ◽  
17Β Estradiol

Estrogens play a crucial role in spermatogenesis and estrogen receptor α knock-out male mice are infertile. It has been demonstrated that estrogens significantly increase the speed of capacitation in vitro; however this may lead to the reduction of reproductive potential due to the decreased ability of these sperm to undergo the acrosome reaction. To date the in vivo effect of estrogens on the ability of sperm to capacitate has not been investigated. Therefore, in this study, we exposed mice (n=24) to 17β-estradiol (E2) at the concentration of 20 ng/ml either during puberty from the fourth to seventh week of age (n=8), or continuously from birth for a period of 12 weeks (n=8) at which age the animals from both groups were killed. The capacitation status of epididymal and testicular sperm was analysed by tyrosine phosphorylation (TyrP) antibody (immunofluorescence and western blot) and chlortetracycline (CTC) assay. According to our results, in vivo exposure to increased E2 concentrations caused premature sperm capacitation in the epididymis. The effect of E2, however, seems reversible because after the termination of the exposure premature epididymal sperm capacitation is decreased in animals treated during puberty. Furthermore the changes in epididymal sperm capacitation status detected by TyrP and CTC positively correlate with plasma levels of E2 and the expression of the estrogen-dependent trefoil factor 1 (Tff1) gene in testicular tissue. Therefore, our data implicate that in vivo exposure to E2 under specific conditions leads to the premature capacitation of mouse sperm in epididymis with a potential negative impact on the sperm reproductive fitness in the female reproductive tract.

In vitro production of cattle-water buffalo (Bos taurus - Bubalus bubalis) hybrid embryos

Zygote ◽  
2002 ◽  
Vol 10 (2) ◽  
pp. 155-162 ◽  
Author(s):  
H.P.S. Kochhar ◽  
K.B.C. Appa Rao ◽  
A.M. Luciano ◽  
S.M. Totey ◽  
F. Gandolfi ◽  
...  
Keyword(s):  
Water Buffalo ◽  
Bos Taurus ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Bubalus Bubalis ◽  
Blastocyst Stage ◽  
In Vitro Production ◽  
Cleavage Rate ◽  
Developmental Potential

Interspecific hybrid embryos are useful models for the study of maternal-fetal interactions, transmission pattern of species-specific markers and parental contributions to growth and developmental potential of pre-attachment embryos. In an attempt to investigate the possibility of producing hybrid embryos of domestic cattle (Bos taurus) and water buffalo (Bubalus bubalis), cattle oocytes were exposed to buffalo sperm and buffalo oocytes were exposed to cattle sperm and the cleavage rate and the post-fertilisation features of hybrid embryos up to the blastocyst stage were compared with those of buffalo and cattle embryos. The cleavage rate in buffalo oocytes exposed to cattle sperm was low (40.8%), with only 8.8% of these hybrid embryos reaching the blastocyst stage. Cattle oocytes exposed to buffalo sperm showed 86.3% cleavage, while 25.9% of these attained the blastocyst stage. The speed of development of both types of hybrids was intermediate between that of cattle and buffalo embryos, with hatching occurring on day 7.5 in hybrid embryos, day 8-9 in cattle and day 7 in buffalo. The proportions of cells contributing to the trophectoderm and the inner cell mass were closer to those of the maternal species in both types of hybrid embryos. Our results indicate that cattle-water buffalo hybrid embryos produced using interspecies gametes are capable of developing to advanced blastocyst stages and that their in vitro fate, and developmental potential, are influenced by the origin of the oocyte.

311 MELATONIN PROMOTES IN VITRO SPERM CAPACITATION IN BUFFALO (BUBALUS BUBALIS)

2010 ◽  
Vol 22 (1) ◽  
pp. 311 ◽  
Author(s):  
S. Di Francesco ◽  
E. Mariotti ◽  
M. Tsantarliotou ◽  
A. Sattar ◽  
I. Venditto ◽  
...  
Keyword(s):  
Acrosome Reaction ◽  
Optimal Dose ◽  
Bubalus Bubalis ◽  
Positive Control ◽  
Negative Control ◽  
Sperm Capacitation ◽  
Chi Square ◽  
Melatonin Administration ◽  
Chi Square Test

Melatonin, the main hormone secreted by the pineal gland, plays many roles in reproduction. In ram spermatozoa, melatonin administration increases plasminogen activator activity (Tsantarliotou MP et al. 2007 Theriogenology 69, 458-465), known to be involved in sperm capacitation and acrosome reaction (Taitzoglou IA et al. 1996 Mol. Androl. 8, 187-197). The aim of this study was to evaluate the efficiency of melatonin to induce buffalo in vitro sperm capacitation, indirectly assessed by estimating the capability of spermatozoa to acrosome-react. Frozen-thawed semen from 4 different bulls was pooled and treated by swim-up in order to select only the motile population. Spermatozoa (n = 829) were assessed immediately after swim- up separation, to evaluate the incidence of acrosomal loss in non-treated cells (time 0). The remaining spermatozoa were incubated in the absence of capacitating agents (negative control; n = 513), in the presence of 0.01 mM heparin (positive control; n = 775), 10 μM melatonin (n = 684), 100 μM melatonin (n = 751), and 1 mM melatonin (n = 650), for 2 h. Sperm were then exposed for 15 min to 60 μg mL-1 of lysophosphatidylcholine, an agent known to induce acrosome reaction (AR) only on capacitated spermatozoa. Trypan blue was used first to differentiate live from dead spermatozoa and the dried smears were then fixed in 37% formaldehyde and stained with Giemsa for acrosome evaluation by microscopic examination. The percentage of acrosome-reacted spermatozoa in each group was used to assess the efficiency of capacitation under different incubation conditions. The experiment was repeated 4 times. Differences among groups were analyzed by chi-square test. Acrosomal loss was observed only in 2.1% of the sperm population at time 0. Interestingly, sperm treatment with both heparin and the different concentrations of melatonin resulted in a significantly higher incidence of AR compared to the negative control (24.4, 20.5, 20.0, 23.6 v. 8.0% for the positive control, 10 μM melatonin, 100 μM melatonin, 1 mM melatonin, and the negative control, respectively; P < 0.01). These results demonstrated that melatonin determines capacitation of buffalo spermatozoa in vitro. Furthermore, the effect of melatonin was comparable to that of heparin, that is, the capacitating agent currently used in the IVF system. The capacitating effect was observed at all the tested concentrations, and viability was not affected. This suggests to extend the range of concentrations to test in future studies, in order to identify the optimal dose. Moreover, considering the seasonality of the species and the great differences in fertility attitude of buffalo bulls, it would be interesting to investigate the capacitation effect of melatonin in relation to both the season and the bull.

scholarly journals El glifosato afecta negativamente a los espermatozoides humanos: evidencia in vitro

2019 ◽  
Vol 29 (02) ◽  
pp. 096-098
Author(s):  
Walter D. Cardona Maya
Keyword(s):  
Reproductive Tract ◽  
Male Reproductive Tract ◽  
Revisión Sistemática

ResumenDeterminar el efecto del glifosato sobre la calidad seminal en humanos a través de una revisión sistemática. Se realizó una búsqueda de la literatura en dos bases de datos (Pubmed y Scopus) de los terminos glyphosate/gliphosate/Roundup/yerbimat y sperm/“male reproductive tract”. Los criterios de selección permitieron analizar tres artículos sobre el efecto del glifosato sobre los espermatoozides humanos. Se observó un efecto de negativo del glifosato sobre los espermatoozides humanos (movilidad, actividad mitocondrial y DNA). Aunque son pocos los artículos que evalúan el efecto del glifosfato sobre la calidad seminal, todos concluyen en sus repercusiones negativas. Investigaciones sobre el tema se requieren especialmente ahora que el Gobierno Nacional pretende implementar su uso.

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