Novel Sperm and Gonadotropin-releasing Hormone-based Recombinant Fusion Protein: Achievement of 100% Contraceptive Efficacy by Co-immunization of Male and Female Mice

2016 ◽  
Vol 83 (12) ◽  
pp. 1048-1059 ◽  
Author(s):  
Vidisha Minhas ◽  
Abhinav Shrestha ◽  
Neerja Wadhwa ◽  
Rita Singh ◽  
Satish Kumar Gupta
Keyword(s):  
Fusion Protein ◽  
Gonadotropin Releasing Hormone ◽  
Recombinant Fusion Protein ◽  
Contraceptive Efficacy ◽  
Male And Female ◽  
Releasing Hormone ◽  
Female Mice

scholarly journals Differential Gene Expression in Gonadotropin-Releasing Hormone Neurons of Male and Metestrous Female Mice

2015 ◽  
Vol 102 (1-2) ◽  
pp. 44-59 ◽  
Author(s):  
Csaba Vastagh ◽  
Annie Rodolosse ◽  
Norbert Solymosi ◽  
Imre Farkas ◽  
Herbert Auer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
Gonadotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Female Mice ◽  
Differential Gene

scholarly journals Peripheral interleukin-1β inhibits arcuate kiss1 cells and LH pulses in female mice

2020 ◽  
Vol 246 (2) ◽  
pp. 149-160
Author(s):  
Katherine N Makowski ◽  
Michael J Kreisman ◽  
Richard B McCosh ◽  
Ali A Raad ◽  
Kellie M Breen
Keyword(s):  
Luteinizing Hormone ◽  
Cell Activation ◽  
Hormone Secretion ◽  
Receptor Activation ◽  
Gonadotropin Releasing Hormone ◽  
Interleukin 1Β ◽  
Luteinizing Hormone Secretion ◽  
Releasing Hormone ◽  
Female Mice ◽  
Neuroendocrine Function

Peripheral immune/inflammatory challenges rapidly disrupt reproductive neuroendocrine function. This inhibition is considered to be centrally mediated via suppression of gonadotropin-releasing hormone secretion, yet the neural pathway(s) for this effect remains unclear. We tested the hypothesis that interleukin-1β inhibits pulsatile luteinizing hormone secretion in female mice via inhibition of arcuate kisspeptin cell activation, a population of neurons considered to be the gonadotropin-releasing hormone pulse generator. In the first experiment, we determined that the inhibitory effect of peripheral interleukin-1β on luteinizing hormone secretion was enhanced by estradiol. We next utilized serial sampling and showed that interleukin-1β reduced the frequency of luteinizing hormone pulses in ovariectomized female mice treated with estradiol. The interleukin-1β-induced suppression of pulse frequency was associated with reduced kisspeptin cell activation, as determined by c-Fos coexpression, but not as a result of impaired responsiveness to kisspeptin challenge. Together, these data suggest an inhibitory action of interleukin-1β upstream of kisspeptin receptor activation. We next tested the hypothesis that estradiol enhances the activation of brainstem nuclei responding to interleukin-1β. We determined that the expression of interleukin-1 receptor was elevated within the brainstem following estradiol. Interleukin-1β induced c-Fos in the area postrema, ventrolateral medulla, and nucleus of the solitary tract; however, the response was not increased by estradiol. Collectively, these data support a neural mechanism whereby peripheral immune/inflammatory stress impairs reproductive neuroendocrine function via inhibition of kisspeptin cell activation and reduced pulsatile luteinizing hormone secretion. Furthermore, these findings implicate the influence of estradiol on peripherally mediated neural pathways such as those activated by peripheral cytokines.

PREOPTIC AREA BRAIN GRAFTS IN HYPOGONADAL (hpg) FEMALE MICE ABOLISH EFFECTS OF CONGENITAL HYPOTHALAMIC GONADOTROPIN-RELEASING HORMONE (GnRH) DEFICIENCY

Endocrinology ◽  
1984 ◽  
Vol 114 (5) ◽  
pp. 1938-1940 ◽  
Author(s):  
M.J. GIBSON ◽  
H.M. CHARLTON ◽  
M.J. PERLOW ◽  
E.A. ZIMMERMAN ◽  
T.F. DAVIES ◽  
...  
Keyword(s):  
Preoptic Area ◽  
Gonadotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Female Mice ◽  
Brain Grafts
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