Lysophosphatidic acid improves porcine oocyte maturation and embryo development in vitro

2015 ◽  
Vol 82 (1) ◽  
pp. 66-77 ◽  
Author(s):  
Jin Yu Zhang ◽  
Yong Jiang ◽  
Tao Lin ◽  
Jung Won Kang ◽  
Jae Eun Lee ◽  
...  
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
Lysophosphatidic Acid ◽  
Porcine Oocyte ◽  
Development In Vitro

Pioglitazone improves porcine oocyte maturation and subsequent parthenogenetic embryo development in vitro by increasing lipid metabolism

2019 ◽  
Vol 86 (9) ◽  
pp. 1245-1254 ◽  
Author(s):  
Sang‐Gi Jeong ◽  
Seung‐Eun Lee ◽  
Won‐Jae Kim ◽  
Yun‐Gwi Park ◽  
Jae‐Wook Yoon ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Embryo Development ◽  
Oocyte Maturation ◽  
Porcine Oocyte ◽  
Parthenogenetic Embryo ◽  
Development In Vitro

260 EVALUATION OF PORCINE IN VITRO PRODUCTION WITH THE ADDITION OF CHITOSAN TO CULTURE MEDIA

2015 ◽  
Vol 27 (1) ◽  
pp. 219 ◽  
Author(s):  
F. García ◽  
Y. Ducolomb ◽  
S. P. Miranda-Castro ◽  
J. F. De la Torre-Sánchez ◽  
S. Romo
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
Culture Media ◽  
Biological Properties ◽  
In Vitro Production ◽  
Porcine Oocyte ◽  
Main Component ◽  
Vitro Production ◽  
Positive Effect

Chitosan is a partially deacetylated polymer obtained from the alkaline deacetylation of chitin, which is a glucose-based unbranched polysaccharide widely distributed in nature as the main component of exoskeletons of crustaceans and insects. Chitosan has a variety of physicochemical and biological properties resulting in numerous applications. In addition to its lack of toxicity and allergenicity, its biocompatibility, biodegradability, and bioactivity make it a very attractive substance for diverse applications as a biomaterial in pharmaceutical and medical fields. Chitosan stimulates cell growth and it has been used in fibroblast culture, increasing cell proliferation. For these reasons, it is important to evaluate if this polymer has a positive effect on embryo production. The aim of this study was to evaluate porcine oocyte maturation and embryo development, comparing the effect of supplementing different concentrations of chitosan to the maturation (MM) and development media (DM). Cumulus-oocyte complexes (COC) were aspirated from ovarian follicles of slaughtered sows. The COC were matured in supplemented TCM-199 (MM) and incubated for 44 h. All incubations were performed at 38.5°C, with 5% CO2 in air and humidity at saturation. After maturation IVF was performed, frozen-thawed semen from the same boar was used and gametes were co-incubated in MTBM for 7 h. Then, putative zygotes were cultured in NCSU-23 (DM) for 144 h. The following experiments were performed: 1) addition of 0 (control), 35, 50, 100, and 150 ppm chitosan to the MM (n = 1353), 2) addition of 0, 50, 100, and 150 ppm chitosan to the DM (n = 739), 3) addition of 0, 50, 100, and 150 ppm of chitosan to the MM first and then the same concentrations to the DM (n = 702). When chitosan was added to the MM, the highest percentage of matured oocytes (metaphase II) was obtained in the 50 ppm treatment (87%, P < 0.05) when compared with the control, 100, and 150 ppm groups (78, 78, and 82%, respectively). Regarding the percentage of blastocysts, there were no differences when comparing the treatment and the control groups (ranging from 12 to 13%). After addition of chitosan to the putative zygotes in the DM, the percentage of morulae in the 150 ppm treatment was significantly increased with regard to the other groups (54 v. 46%, respectively, P < 0.05). When adding chitosan to both MM and DM, there was no effect on embryo development. It is concluded that the addition of chitosan to the MM at a concentration of 50 ppm significantly improved oocyte maturation and a concentration of 150 ppm in the DM increased the percentage of morulae. Chitosan had a positive effect on oocyte maturation and embryo development. These results justify further investigations to find out if chitosan can be useful as a supplement for chemically defined media.

scholarly journals Oocyte maturation, fertilization and embryo development in vitro and in vivo in the gaur (Bos gaurus)

Reproduction ◽  
1994 ◽  
Vol 100 (1) ◽  
pp. 131-136 ◽  
Author(s):  
L. A. Johnston ◽  
J. J. Parrish ◽  
R. Monson ◽  
L. Leibfried-Rutledge ◽  
J. L. Susko-Parrish ◽  
...  
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
Bos Gaurus ◽  
Development In Vitro

scholarly journals 91IMPACT OF PROPYLENE GLYCOL V. ETHYLENE GLYCOL ON OOCYTE MATURATION, SPINDLE INTEGRITY, FERTILIZATION AND EMBRYO DEVELOPMENT IN VITRO IN THE DOMESTIC CAT

2004 ◽  
Vol 16 (2) ◽  
pp. 167
Author(s):  
P. Comizzoli ◽  
D.E. Wildt ◽  
B.S. Pukazhenthi
Keyword(s):  
Ethylene Glycol ◽  
Propylene Glycol ◽  
Embryo Development ◽  
Oocyte Maturation ◽  
Domestic Cat ◽  
Hoechst 33342 ◽  
Immature Oocytes ◽  
Development In Vitro ◽  
The Impact

A thorough characterization of cryoprotectant (CPA) sensitivity is required to formulate a successful cryopreservation protocol for any biomaterial. The aim of this study was to characterize the toxic impact of various CPA types, concentrations, and exposure temperatures on the immature domestic cat oocyte. In Experiment 1, grade I immature oocytes (n=561) were exposed (30min; 25°C or 0°C) to 0M, 0.75M, 1.5M, or 3M of propylene glycol (PrOH) or ethylene glycol (EG) in PBS+20% fetal calf serum (v/v). After exposure, CPA was removed step-wise by subjecting oocytes to decreased CPA concentrations. Oocytes were cultured (30h; 38.5°C, 5% CO2) in IVM medium as reported previously (Wolfe and Wildt 1996 J. Reprod. Fertil. 106, 135–141). Oocytes were then fixed and stained to examine nuclear status (Hoechst 33342) and spindle integrity (FITC-labeled anti-α-tubulin antibodies; Sigma Chemical Co., St. Louis, MO). Experiment 2 was designed on the basis of Experiment 1 results to assess the impact of the spindle abnormalities on subsequent embryo development. Oocytes (n=776) were exposed to CPA conditions yielding optimal nuclear maturation with either high (0.75M or 3.0M PrOH or 1.5M EG at 25°C) or low (1.5M PrOH at 25°C) proportions of abnormal spindle. After IVM, oocytes were inseminated with thawed semen (5×105 motile sperm mL−1 ) in Ham’s F-10 (Irvine Scientific, St-Anna, CA). At 16h post-insemination, oocytes were fixed and stained (Hoechst 33342) to assess IVF success (pronuclear formation) or cultured in vitro for 7 days to assess embryo development. Data were analyzed by ANOVA and Tukey’s multiple comparison test. In Experiment 1, CPA treatment had no effect (NS) on meiotic progression to metaphase I. However, percentage of oocytes reaching metaphase II (MII) was reduced (P&lt;0.05) in 3.0M PrOH at 0°C (29.3±8.3%; mean±SD), 3.0M EG at 25°C (33.7±8.9%), and 0°C (29.4±11.0%) compared to all other conditions examined (range, 52.0% to 62.0%). All CPA treatments also increased (P&lt;0.05) spindle abnormalities at MII (range, 40.3% to 75.9%) compared to control (13.8±8.6%), except 1.5M PrOH at 25°C (20.7±10.1%). None of the CPA treatments in Experiment 2 influenced IVF success (range, 55% to 63%; NS). However, percentage of cleaved embryos was reduced (P&lt;0.05) in 0.75M PrOH (32.1±4.1%), 1.5M EG (33.4±4.0%), and 3.0M PrOH (29.3±3.8%) compared to control (50.1±4.0%) or 1.5M PrOH (50.6±4.9%). Developmental competence (number of blastocysts relative to number of cleaved embryos) also was impaired (P&lt;0.05) in 1.5M EG (16.5±7.4%) and 3.0M PrOH (14.9±7.8%) compared to the other conditions (range, 32.5% to 38.5%), including 1.5 PrOH at 25°C (32.5±7.8%). In conclusion, exposure of immature oocytes to 1.5M PrOH at 25°C does not adversely impact oocyte maturation, MII spindle, fertilization, or embryo development in vitro in the domestic cat.

scholarly journals Exogenous paraoxonase-1 during oocyte maturation improves bovine embryo development in vitro

2016 ◽  
Vol 51 (5) ◽  
pp. 827-830 ◽  
Author(s):  
JAA Rincón ◽  
EM Madeira ◽  
FT Campos ◽  
B Mion ◽  
JF Silva ◽  
...  
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
Paraoxonase 1 ◽  
Bovine Embryo ◽  
Development In Vitro
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