Supplementation of bovine embryo culture medium with L -arginine improves embryo quality via nitric oxide production

2014 ◽  
Vol 81 (10) ◽  
pp. 918-927 ◽  
Author(s):  
Priscila Di Paula Bessa Santana ◽  
Thiago Velasco Guimarães Silva ◽  
Nathália Nogueira da Costa ◽  
Bruno Barauna da Silva ◽  
Timothy Frederick Carter ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Embryo Culture ◽  
Culture Medium ◽  
Embryo Quality ◽  
Bovine Embryo ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Embryo Culture Medium

Oxygen tension modulates extracellular vesicles and its miRNA contents in bovine embryo culture medium

2019 ◽  
Vol 86 (8) ◽  
pp. 1067-1080 ◽  
Author(s):  
Gabriella Mamede Andrade ◽  
Monalisa Medrado Bomfim ◽  
Maite del Collado ◽  
Flávio Vieira Meirelles ◽  
Felipe Perecin ◽  
...  
Keyword(s):  
Oxygen Tension ◽  
Extracellular Vesicles ◽  
Embryo Culture ◽  
Culture Medium ◽  
Bovine Embryo ◽  
Embryo Culture Medium

Embryo quality assessment by the small noncoding RNA expression profile in an embryo culture medium in assisted reproductive technology programs

2019 ◽  
Vol 6_2019 ◽  
pp. 78-86 ◽  
Author(s):  
Timofeeva A.V. Timofeeva ◽  
Kalinina E.A. Kalinina ◽  
Drapkina Yu.S. Drapkina ◽  
Chagovets V.V. Chagovets ◽  
Makarova N.P. Makarova ◽  
...  
Keyword(s):  
Quality Assessment ◽  
Assisted Reproductive Technology ◽  
Embryo Culture ◽  
Noncoding Rna ◽  
Culture Medium ◽  
Embryo Quality ◽  
Reproductive Technology ◽  
Rna Expression ◽  
Small Noncoding Rna ◽  
Embryo Culture Medium

scholarly journals Isolation and Characterization of Functionally Active Extracellular Vesicles from Culture Medium Conditioned by Bovine Embryos In Vitro

2018 ◽  
Vol 20 (1) ◽  
pp. 38 ◽  
Author(s):  
Krishna Pavani ◽  
An Hendrix ◽  
Wim Van Den Broeck ◽  
Liesbeth Couck ◽  
Katarzyna Szymanska ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Embryo Culture ◽  
Culture Medium ◽  
Culture Media ◽  
Apoptotic Cell ◽  
Bovine Embryo ◽  
Bovine Embryos ◽  
Isolation And Characterization ◽  
Embryo Culture Medium

Extracellular vesicles (EVs) play a possible role in cell–cell communication and are found in various body fluids and cell conditioned culture media. The aim of this study was to isolate and characterize EVs in culture medium conditioned by bovine embryos in group and to verify if these EVs are functionally active. Initially, ultracentrifuged bovine serum albumin (BSA) containing medium was selected as suitable EV-free embryo culture medium. Next, EVs were isolated from embryo conditioned culture medium by OptiPrepTM density gradient ultracentrifugation. Isolated EVs were characterized by nanoparticle tracking analysis, western blotting, transmission, and immunoelectron microscopy. Bovine embryo-derived EVs were sizing between 25–230 nm with an average concentration of 236.5 ± 1.27 × 108 particles/mL. Moreover, PKH67 EV pre-labeling showed that embryo-secreted EVs were uptaken by zona-intact bovine embryos. Since BSA did not appear to be a contaminating EV source in culture medium, EV functionality was tested in BSA containing medium. Individual embryo culture in BSA medium enriched with EVs derived from conditioned embryo culture medium showed significantly higher blastocyst rates at day 7 and 8 together with a significantly lower apoptotic cell ratio. In conclusion, our study shows that EVs play an important role in inter embryo communication during bovine embryo culture in group.

502: Pressure Induces Nitric Oxide Production by Human Ureteral Cells in Vitro

2005 ◽  
Vol 173 (4S) ◽  
pp. 137-137
Author(s):  
Michael M. Ohebshalom ◽  
Stella K. Maeng ◽  
Jie Chen ◽  
Dix P. Poppas ◽  
Diane Felsen
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Production ◽  
Oxide Production

Effect of Nelumbo nucifera on nitric oxide production and co-stimulatory molecules

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
D Mukherjee ◽  
TN Khatua ◽  
A Biswas ◽  
T Biswas ◽  
BP Saha ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nelumbo Nucifera ◽  
Nitric Oxide Production ◽  
Oxide Production
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