cDNA cloning, localization, and candidate binding partners of acid-extractable vitelline-coat protein Ci-v-Themis-like in the ascidian Ciona intestinalis

2013 ◽  
Vol 80 (10) ◽  
pp. 840-848 ◽  
Author(s):  
Kei Otsuka ◽  
Lixy Yamada ◽  
Hitoshi Sawada
Keyword(s):  
Coat Protein ◽  
Cdna Cloning ◽  
Ciona Intestinalis ◽  
Binding Partners ◽  
Vitelline Coat

scholarly journals Localization of the Transglutaminase Cross-Linking Sites in the Bacillus subtilis Spore Coat Protein GerQ

2006 ◽  
Vol 188 (21) ◽  
pp. 7609-7616 ◽  
Author(s):  
Alicia Monroe ◽  
Peter Setlow
Keyword(s):  
Bacillus Subtilis ◽  
Coat Protein ◽  
Cross Linking ◽  
Spore Coat ◽  
Bacillus Subtilis Spore ◽  
Binding Partners ◽  
Lysine Residues ◽  
Cross Links ◽  
Hydrolysis Of ◽  
Spore Coat Protein

ABSTRACT The Bacillus subtilis spore coat protein GerQ is necessary for the proper localization of CwlJ, an enzyme important in the hydrolysis of the peptidoglycan cortex during spore germination. GerQ is cross-linked into high-molecular-mass complexes in the spore coat late in sporulation, and this cross-linking is largely due to a transglutaminase. This enzyme forms an ε-(γ-glutamyl) lysine isopeptide bond between a lysine donor from one protein and a glutamine acceptor from another protein. In the current work, we have identified the residues in GerQ that are essential for transglutaminase-mediated cross-linking. We show that GerQ is a lysine donor and that any one of three lysine residues near the amino terminus of the protein (K2, K4, or K5) is necessary to form cross-links with binding partners in the spore coat. This leads to the conclusion that all Tgl-dependent GerQ cross-linking takes place via these three lysine residues. However, while the presence of any of these three lysine residues is essential for GerQ cross-linking, they are not essential for the function of GerQ in CwlJ localization.

A fucosyl glycoprotein component with sperm receptor and sperm-activating activities from the vitelline coat of ciona intestinalis eggs

1983 ◽  
Vol 148 (2) ◽  
pp. 508-513 ◽  
Author(s):  
R.De Santis ◽  
M.R. Pinto ◽  
F. Cotelli ◽  
F. Rosati ◽  
A. Monroy ◽  
...  
Keyword(s):  
Ciona Intestinalis ◽  
Vitelline Coat ◽  
Sperm Receptor

cDNA cloning of artichoke mottled crinkle virus RNA and localization and sequencing of the coat protein gene

1989 ◽  
Vol 13 (6) ◽  
pp. 685-692 ◽  
Author(s):  
Mario Tavazza ◽  
Alessandra Lucioli ◽  
Giorgio Ancora ◽  
Eugenio Benvenuto
Keyword(s):  
Coat Protein ◽  
Cdna Cloning ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Virus Rna
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