Replacement of bovine serum albumin with N -methyl-D -aspartic acid and homocysteine improves development, but not live birth

2012 ◽  
Vol 79 (5) ◽  
pp. 310-310 ◽  
Author(s):  
L.D. Spate ◽  
B.K. Redel ◽  
A.N. Brown ◽  
C.N. Murphy ◽  
R.S. Prather
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Aspartic Acid ◽  
Live Birth ◽  
Bovine Serum

scholarly journals Sequence of residues 400–403 of bovine serum albumin

1980 ◽  
Vol 191 (3) ◽  
pp. 867-868 ◽  
Author(s):  
R G Reed ◽  
F W Putnam ◽  
T Peters
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Aspartic Acid ◽  
Bovine Serum ◽  
Tryptic Peptide ◽  
Edman Degradation ◽  
Protein Residues

A large tryptic peptide of bovine serum albumin (residues 377–582) was subjected to 32 cycles of Edman degradation to determine the sequence of the last remaining unknown segment of this protein. Residues 400–403 were identified as gly-Phe-Gln-Asn. Amide assignments were also made at positions 388 (glutamine), 389 (asparagine), 391 (aspartic acid) and 392 (glutamine).

Self-assembling of poly(aspartic acid) with bovine serum albumin in aqueous solutions

2017 ◽  
Vol 95 ◽  
pp. 412-420 ◽  
Author(s):  
L.E. Nita ◽  
A.P. Chiriac ◽  
M. Bercea ◽  
M. Asandulesa ◽  
Bernhard A. Wolf
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Aqueous Solutions ◽  
Aspartic Acid ◽  
Bovine Serum ◽  
Self Assembling

scholarly journals Tailorable polyelectrolyte protein complex based on poly(aspartic acid) and bovine serum albumin

2016 ◽  
Vol 19 (7) ◽  
pp. 596-606 ◽  
Author(s):  
Loredana E. Nita ◽  
Aurica P. Chiriac ◽  
Elena Stoleru ◽  
Alina Diaconu ◽  
Nita Tudorachi
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Aspartic Acid ◽  
Protein Complex ◽  
Bovine Serum

128 N-METHYL-D-ASPARTIC ACID CAN BE USED TO PARTIALLY REPLACE BOVINE SERUM ALBUMIN IN CULTURE MEDIUM

2009 ◽  
Vol 21 (1) ◽  
pp. 164
Author(s):  
L. D. Spate ◽  
B. K. Bauer ◽  
C. N. Murphy ◽  
R. S. Prather
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Aspartic Acid ◽  
Bovine Serum ◽  
Cell Number ◽  
Total Cell ◽  
Total Cell Number ◽  
Treatment Groups ◽  
Chemically Defined Media ◽  
Defined Media

One major obstacle in mammalian embryo culture has been unidentifiable biological contaminants in the media due to the inclusion of Bovine Serum Albumin (BSA) or Fetal Bovine Serum. The goal of this study was to remove BSA from culture media and develop chemically defined media based off the embryo’s biological and physiologic makeup. We evaluated the presence of message in various stages of porcine embryos and found that the message for the ionic glutamate receptor, N-Methyl-D-aspartic acid (NMDA) increased about 3-fold from oocyte to blastocyst. Thus, this study was conducted to determine if the addition of NMDA (0.5 mm) would improve development of embryos in an already chemically defined medium. Slaughterhouse derived ovaries were aspirated, cumulus–oocyte complexes were identified and then matured for 42 h in M199 base medium supplemented with EGF, FSH, and LH. Metaphase II oocytes were selected and fertilized in modified Tris buffered medium with 0.25 × 106 mL–1 frozen–thawed porcine semen for 5 h. Presumptive zygotes were then transferred to Porcine Zygote Medium with 0.3% BSA (PZM3) or 0.1% PVA (PZM4). After 28 h, cleaved embryos were selected and embryos were placed into treatment groups: (1) PZM3, (2) PZM4, or (3) PZM4 + 0.5 mm NMDA. Embryos were cultured in 5% CO2, 5%O2, 90% N2 until Day 7. For this experiment the number of cleaved embryos cultured in each treatment group were 260 for group 1, 220 for group 2 and 300 for group 3. Percentage of development to blastocyst was determined and analyzed with SAS Proc GENMOD Procedure (a,b P < 0.05). The percentage developed to blastocyst was (1) 47.5% a, (2) 29.6% b, and (3) 36.1% a,b, respectively. Total cell number of the blastocysts was determined by using Hoechst nuclear stain and statistically analyzed by SAS Proc GENMOD Procedure. The average cell number for the treatment groups was (1) 25.8 a, (2) 19.6 b, and (3) 22.9 a,b, respectively. Culture without BSA significantly reduced development to blastocyst and total cell number; however, with the addition of 0.5 mm NMDA there was no significant difference from media containing BSA. This indicates that NMDA can be used to partially replace BSA to form a chemically defined media. Funded by a grant from the USDA NRI 2006-35203-17282.

Magnetic composites based on bovine serum albumin and poly(aspartic acid)

2019 ◽  
Vol 59 (7) ◽  
pp. 1409-1415 ◽  
Author(s):  
Alina Ghilan ◽  
Aurica P. Chiriac ◽  
Loredana E. Nita
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Aspartic Acid ◽  
Bovine Serum ◽  
Magnetic Composites

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

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