Identification and characterization of Wfdc gene expression in the male reproductive tract of the rat

2011 ◽  
Vol 78 (9) ◽  
pp. 633-641 ◽  
Author(s):  
Angireddy Rajesh ◽  
Golla Madhubabu ◽  
Suresh Yenugu
Keyword(s):  
Gene Expression ◽  
Reproductive Tract ◽  
Male Reproductive Tract ◽  
Identification And Characterization

scholarly journals A Novel Triple-Fluorescent HCMV Strain Reveals Gene Expression Dynamics and Anti-Herpesviral Drug Mechanisms

2021 ◽  
Vol 10 ◽  
Author(s):  
Ulfert Rand ◽  
Tobias Kubsch ◽  
Bahram Kasmapour ◽  
Luka Cicin-Sain
Keyword(s):  
Gene Expression ◽  
Hcmv Infection ◽  
Viral Fitness ◽  
High Temporal Resolution ◽  
Detection Techniques ◽  
Drug Candidates ◽  
New Drug ◽  
Gene Expression Dynamics ◽  
Identification And Characterization

Human Cytomegalovirus (HCMV) infection may result in severe outcomes in immunocompromised individuals such as AIDS patients, transplant recipients, and neonates. To date, no vaccines are available and there are only few drugs for anti-HCMV therapy. Adverse effects and the continuous emergence of drug-resistance strains require the identification of new drug candidates in the near future. Identification and characterization of such compounds and biological factors requires sensitive and reliable detection techniques of HCMV infection, gene expression and spread. In this work, we present and validate a novel concept for multi-reporter herpesviruses, identified through iterative testing of minimally invasive mutations. We integrated up to three fluorescence reporter genes into replication-competent HCMV strains, generating reporter HCMVs that allow the visualization of replication cycle stages of HCMV, namely the immediate early (IE), early (E), and late (L) phase. Fluorescent proteins with clearly distinguishable emission spectra were linked by 2A peptides to essential viral genes, allowing bicistronic expression of the viral and the fluorescent protein without major effects on viral fitness. By using this triple color reporter HCMV, we monitored gene expression dynamics of the IE, E, and L genes by measuring the fluorescent signal of the viral gene-associated fluorophores within infected cell populations and at high temporal resolution. We demonstrate distinct inhibitory profiles of foscarnet, fomivirsen, phosphonoacetic acid, ganciclovir, and letermovir reflecting their mode-of-action. In conclusion, our data argues that this experimental approach allows the identification and characterization of new drug candidates in a single step.

scholarly journals Identification and characterization of an injury-induced skeletal progenitor

2015 ◽  
Vol 112 (32) ◽  
pp. 9920-9925 ◽  
Author(s):  
Owen Marecic ◽  
Ruth Tevlin ◽  
Adrian McArdle ◽  
Eun Young Seo ◽  
Taylor Wearda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Progenitor Cells ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Cell Type ◽  
Fracture Callus ◽  
Adult Mice ◽  
Induced Changes ◽  
Identification And Characterization

The postnatal skeleton undergoes growth, remodeling, and repair. We hypothesized that skeletal progenitor cells active during these disparate phases are genetically and phenotypically distinct. We identified a highly potent regenerative cell type that we term the fracture-induced bone, cartilage, stromal progenitor (f-BCSP) in the fracture callus of adult mice. The f-BCSP possesses significantly enhanced skeletogenic potential compared with BCSPs harvested from uninjured bone. It also recapitulates many gene expression patterns involved in perinatal skeletogenesis. Our results indicate that the skeletal progenitor population is functionally stratified, containing distinct subsets responsible for growth, regeneration, and repair. Furthermore, our findings suggest that injury-induced changes to the skeletal stem and progenitor microenvironments could activate these cells and enhance their regenerative potential.

scholarly journals Drosophila female reproductive tract gene expression reveals coordinated mating responses and rapidly evolving tissue-specific genes

2021 ◽  
Author(s):  
Caitlin E McDonough-Goldstein ◽  
Kirill Borziak ◽  
Scott Pitnick ◽  
Steve Dorus
Keyword(s):  
Gene Expression ◽  
Reproductive Tract ◽  
Expression Profiles ◽  
Female Reproductive Tract ◽  
Tissue Specific ◽  
Accelerated Evolution ◽  
Before And After ◽  
Complex Coordination ◽  
Extracellular Environment

Abstract Sexual reproduction in internally fertilizing species requires complex coordination between female and male reproductive systems and among the diverse tissues of the female reproductive tract (FRT). Here, we report a comprehensive, tissue-specific investigation of Drosophila melanogaster FRT gene expression before and after mating. We identified expression profiles that distinguished each tissue, including major differences between tissues with glandular or primarily non-glandular epithelium. All tissues were enriched for distinct sets of genes possessing secretion signals and exhibiting accelerated evolution, as might be expected for genes participating in molecular interactions between the sexes within the FRT extracellular environment. Despite robust transcriptional differences between tissues, post-mating responses were dominated by coordinated transient changes indicative of an integrated systems-level functional response. This comprehensive characterization of gene expression throughout the FRT identifies putative female contributions to post-copulatory events critical to reproduction and potentially reproductive isolation, as well as the putative targets of sexual selection and conflict.

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