scholarly journals Role of AMPK throughout meiotic maturation in the mouse oocyte: Evidence for promotion of polar body formation and suppression of premature activation

2010 ◽  
Vol 77 (10) ◽  
pp. 888-899 ◽  
Author(s):  
Stephen M. Downs ◽  
Ru Ya ◽  
Christopher C. Davis
Keyword(s):  
Polar Body ◽  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Body Formation ◽  
Polar Body Formation

scholarly journals Role of Securin, Separase and Cohesins in female meiosis and polar body formation inDrosophila

2015 ◽  
Vol 129 (3) ◽  
pp. 531-542 ◽  
Author(s):  
Zhihao Guo ◽  
Osamah Batiha ◽  
Mohammed Bourouh ◽  
Eric Fifield ◽  
Andrew Swan
Keyword(s):  
Polar Body ◽  
Female Meiosis ◽  
Body Formation ◽  
Polar Body Formation

Memoirs: The Germ-Cell Cycle in Phytophaga destructor Say

1935 ◽  
Vol s2-77 (308) ◽  
pp. 585-604
Author(s):  
MARGOT E. METEALFE
Keyword(s):  
Germ Cells ◽  
Growth Stage ◽  
Germ Line ◽  
Polar Body ◽  
Metaphase Plate ◽  
Body Formation ◽  
Polar Bodies ◽  
Complicated Process ◽  
Female Pronucleus ◽  
Polar Body Formation

1. The somatic cells in both sexes of Phytophaga destructor Say contain four pairs of V-shaped chromosomes, the sex-group being indistinguishable in size or form. 2. The germ-cells in both sexes contain eight pairs of chromosomes. 3. The maturation of the egg follows the normal course of development, eight bivalents being formed. After polar body formation the female pronucleus has eight chromosomes. The polar bodies are never extruded from the egg. 4. Spermatogenesis is a complicated process, the details of which have not been satisfactorily determined. The growth stage appears to take place before the last spermatogonial division. No pairing of chromosome has been observed, and apparently no metaphase plate is formed at meiosis. Eeduction is effected by the expulsion of two buds each containing four chromosomes. Thus only one sperm is produced from each spermatocyte. 5. One or more sperms may enter the egg at fertilization. 6. The germ-line is differentiated from the soma at the eightcell stage. 7. At the fifth cleavage the somatic nuclei eliminate half their number of chromosomes, and are left with eight chromosomes. 8. Migration of the germ nuclei takes place at the sixteencell stage. 9. The relation of the chromosome numbers in the somatic and germ lines is discussed.

Preimplantation development of gynogenetic diploid mouse embryos

Development ◽  
1982 ◽  
Vol 69 (1) ◽  
pp. 215-222
Author(s):  
Ewa Borsuk
Keyword(s):  
Cytochalasin B ◽  
Polar Body ◽  
Mouse Embryos ◽  
Fertilization In Vitro ◽  
Body Formation ◽  
Male Pronucleus ◽  
Step Procedure ◽  
Gynogenetic Diploid ◽  
Polar Body Formation

Diploid gynogenetic mouse embryos were produced in a three-step procedure: fertilization in vitro, suppression of the 2nd polar body formation by Cytochalasin B, and microsurgical removal of the male pronucleus. The operated eggs were transplanted to the oviduct of recipient females for 72 or 96 h. The overall recovery rate was 73%, but compacted morulae and blastocysts constituted only 28·6% of transplanted eggs. After 72 h blastocysts were rare (3·5%) but 24 h later their incidence increased to 21·2%. In eggs homozygous for T6 chromosome it was possible to prove karyologically that the male pronucleus was effectively removed and that the diploid genome was of purely maternal origin.

JNK2 Participates in Spindle Assembly during Mouse Oocyte Meiotic Maturation

2011 ◽  
Vol 17 (2) ◽  
pp. 197-205 ◽  
Author(s):  
Xin Huang ◽  
Jing-Shan Tong ◽  
Zhen-Bo Wang ◽  
Cai-Rong Yang ◽  
Shu-Tao Qi ◽  
...  
Keyword(s):  
Polar Body ◽  
Specific Inhibitor ◽  
Germinal Vesicle ◽  
Spindle Assembly ◽  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Cytoplasmic Microtubule ◽  
Spindle Poles ◽  
First Polar Body ◽  
Oocyte Meiotic Maturation

AbstractIt is well known that c-Jun N-terminal kinase (JNK) plays pivotal roles in various mitotic events, but its function in mammalian oocyte meiosis remains unknown. In this study, we found that no specific JNK2 signal was detected in germinal vesicle stage. JNK2 was associated with the spindles especially the spindle poles and cytoplasmic microtubule organizing centers at prometaphase I, metaphase I, and metaphase II stages. JNK2 became diffusely distributed and associated with the midbody at telophase I stage. Injection of myc-tagged JNK2α1 mRNA into oocytes also revealed its localization on spindle poles. The association of JNK2 with spindle poles was further confirmed by colocalization with the centrosomal proteins, γ-tubulin and Plk1. Nocodazole treatment showed that JNK2 may interact with Plk1 to regulate the spindle assembly. Then we investigated the possible function of JNK2 by JNK2 antibody microinjection and JNK specific inhibitor SP600125 treatment. These two manipulations caused abnormal spindle formation and decreased the rate of first polar body (PB1) extrusion. In addition, inhibition of JNK2 resulted in impaired localization of Plk1. Taken together, our results suggest that JNK2 plays an important role in spindle assembly and PB1 extrusion during mouse oocyte meiotic maturation.

Induction of Tetraploidy in Mussels by Suppression of Polar Body Formation.

1993 ◽  
Vol 59 (12) ◽  
pp. 2017-2023 ◽  
Author(s):  
John Scarpa ◽  
Katsuhiko T.Wada ◽  
Akira Komaru
Keyword(s):  
Polar Body ◽  
Body Formation ◽  
Polar Body Formation

Hydrozoan eggs can only be fertilized at the site of polar body formation

1982 ◽  
Vol 94 (1) ◽  
pp. 142-152 ◽  
Author(s):  
Gary Freeman ◽  
Richard L. Miller
Keyword(s):  
Polar Body ◽  
Body Formation ◽  
Polar Body Formation
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