Steady-state level of kit ligand mRNA in goat ovaries and the role of kit ligand in preantral follicle survival and growth in vitro

2009 ◽  
Vol 77 (3) ◽  
pp. 231-240 ◽  
Author(s):  
Juliana J.H. Celestino ◽  
Jamily B. Bruno ◽  
Isabel B. Lima-Verde ◽  
Maria Helena T. Matos ◽  
Mércia Viviane A. Saraiva ◽  
...  
Keyword(s):  
Steady State ◽  
State Level ◽  
Steady State Level ◽  
Preantral Follicle ◽  
Kit Ligand ◽  
Survival And Growth

Cytosolic Ca2+ dynamics in hamster ascending thin limb of Henle's loop

1995 ◽  
Vol 268 (6) ◽  
pp. F1148-F1153 ◽  
Author(s):  
N. Takahashi ◽  
Y. Kondo ◽  
O. Ito ◽  
Y. Igarashi ◽  
K. Omata ◽  
...  
Keyword(s):  
Steady State ◽  
Intracellular Calcium ◽  
State Level ◽  
Steady State Level ◽  
Subsequent Reduction ◽  
Fura 2 ◽  
Henle's Loop ◽  
K Concentration ◽  
Thin Limb

Intracellular calcium plays an important role in the regulation of Cl- reabsorption in the ascending thin limb of Henle's loop (ATL). To elucidate the cytosolic Ca2+ dynamics in the ATL, intracellular Ca2+ concentration activity ([Ca2+]i) was measured in the in vitro microperfused hamster ATL using fura 2. Basal [Ca2+]i was 89.1 +/- 7.3 nM (n = 9 tubules). Removal of Ca2+ from the peritubular solution decreased [Ca2+]i from 89.1 +/- 7.3 to 64.1 +/- 7.1 nM in 2 min (n = 9, P < 0.05), whereas [Ca2+]i did not change after removal of Ca2+ from the luminal solution. Addition of 1 mM NaCN to the bath increased [Ca2+]i. This effect was completely abolished by the elimination of ambient Ca2+. Trifluoperazine and N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7) in the bath reversibly increased [Ca2+]i, whereas addition of 1 mM ouabain to the bath decreased [Ca2+]i. Rates of changes in [Ca2+]i after removal and replacement of basolateral Ca2+ were not affected by removal of Na+, K+, or Cl- from the bath, whereas nicardipine decreased these parameters. Increasing bath K+ from 5 to 100 mM decreased [Ca2+]i from 69.3 +/- 5.8 to 50.8 +/- 5.0 nM in 1 min (n = 6, P < 0.05). Subsequent reduction of K+ from 100 to 5 mM increased [Ca2+]i to 174.0 +/- 30.8 nM in 1 min, followed by a gradual decrease in [Ca2+]i to a steady-state level of 74.2 +/- 8.0 nM in 2 min. Changes in basolateral K+ concentration did not affect [Ca2+]i in the absence of ambient Ca2+.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Steady-state level of messenger RNA and immunolocalization of aquaporins 3, 7, and 9 during in vitro growth of ovine preantral follicles

2015 ◽  
Vol 84 (1) ◽  
pp. 1-10 ◽  
Author(s):  
A.D. Sales ◽  
A.B.G. Duarte ◽  
G.Q. Rodrigues ◽  
L.F. Lima ◽  
G.M. Silva ◽  
...  
Keyword(s):  
Steady State ◽  
Messenger Rna ◽  
State Level ◽  
In Vitro Growth ◽  
Steady State Level ◽  
Preantral Follicles

Steady-state level of epidermal growth factor (EGF) mRNA and effect of EGF on in vitro culture of caprine preantral follicles

2011 ◽  
Vol 344 (3) ◽  
pp. 539-550 ◽  
Author(s):  
Juliana Jales H. Celestino ◽  
Jamily B. Bruno ◽  
Márcia Viviane A. Saraiva ◽  
Rebeca M. P. Rocha ◽  
Ivina R. Brito ◽  
...  
Keyword(s):  
Steady State ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
In Vitro Culture ◽  
State Level ◽  
Steady State Level ◽  
Preantral Follicles ◽  
Epidermal Growth

scholarly journals A Pathogenic CytochromebMutation Reveals New Interactions between Subunits of the Mitochondrialbc1Complex

2002 ◽  
Vol 277 (51) ◽  
pp. 49397-49402 ◽  
Author(s):  
Yann Saint-Georges ◽  
Nathalie Bonnefoy ◽  
Jean Paul di Rago ◽  
Stephane Chiron ◽  
Geneviève Dujardin
Keyword(s):  
Steady State ◽  
High Temperature ◽  
State Level ◽  
Energy Transduction ◽  
Steady State Level ◽  
Catalytic Subunits ◽  
Suppressor Mutations ◽  
Human Cytochrome ◽  
New Interactions

Energy transduction in mitochondria involves five oligomeric complexes embedded within the inner membrane. They are composed of catalytic and noncatalytic subunits, the role of these latter proteins often being difficult to assign. One of these complexes, thebc1complex, is composed of three catalytic subunits including cytochromeband seven or eight noncatalytic subunits. Recently, several mutations in the human cytochromebgene have been linked to various diseases. We have studied in detail the effects of a cardiomyopathy generating mutationG252Din yeast. This mutation disturbs the biogenesis of thebc1complex at 36 °C and decreases the steady-state level of the noncatalytic subunit Qcr9p. In addition, theG252Dmutation and the deletion ofQCR9show synergetic defects that can be partially bypassed by suppressor mutations at position 252 and by a new cytochromebmutation,P174T. Altogether, our results suggest that the supernumerary subunit Qcr9p enhances or stabilizes the interactions between the catalytic subunits, this role being essential at high temperature.

scholarly journals Steady-state level of insulin-like growth factor-I (IGF-I) receptor mRNA and the effect of IGF-I on the in vitro culture of caprine preantral follicles

2012 ◽  
Vol 77 (1) ◽  
pp. 206-213 ◽  
Author(s):  
D.M. Magalhães-Padilha ◽  
A.B.G. Duarte ◽  
V.R. Araújo ◽  
M.V.A. Saraiva ◽  
A.P. Almeida ◽  
...  
Keyword(s):  
Steady State ◽  
Growth Factor ◽  
In Vitro Culture ◽  
State Level ◽  
Steady State Level ◽  
Preantral Follicles ◽  
Factor I ◽  
Receptor Mrna ◽  
Igf I

The role of benzoquinones in the electron transport system

1963 ◽  
Vol 157 (968) ◽  
pp. 355-366 ◽  
Keyword(s):  
Steady State ◽  
Electron Transport ◽  
State Level ◽  
Biologically Active ◽  
Transport Systems ◽  
Steady State Level ◽  
Ciba Foundation Symposium ◽  
Photo Oxidation ◽  
The Past

During the past few years two new classes of benzoquinones have been added to the list of biologically active quinone compounds, namely the ubiquinones and the plastoquinones. Consideration has also been given to the possible functions of the vitamins tocopherol and vitamin K in electron-transport systems (Slater 1959; Martius 1960); but contradictory views have not so far been reconciled. The importance of ubiquinones was quickly recognized and indeed it was their participation in oxidative phosphorylation which led to their discovery (Ciba Foundation Symposium 1960). In accordance with the value of their redox potential they link the succinate and the NADH oxidation systems to the cytochrome chain (Green 1962). At the time when identification of the ubiquinones was proceeding, a different benzoquinone was found in leaves (Lester & Crane 1959). Its structure proved to be dimethyl-solanosyl-benzoquinone (Trenner, leads to an increase in the steady-state level of in the leaf, and on removal of light this steady-state level falls to the value previously recorded in the darkened leaf. The photo-oxidation of ascorbic acid is suppressed on poisoning the leaf with cyanide, and at the same time the steady-state level of is enhanced in the darkened leaf. With such poisoned leaves light stimulates the reduction of DHA , and the rate again depends on the intensity of illumination.

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