The proximal promoter governs germ cell-specific expression of the mouse glutathione transferasemGstm5gene

2009 ◽  
Vol 76 (4) ◽  
pp. 379-388 ◽  
Author(s):  
Hironari Dehari ◽  
Tatyana Tchaikovskaya ◽  
Eugeny Rubashevsky ◽  
Rani Sellers ◽  
Irving Listowsky
Keyword(s):  
Germ Cell ◽  
Proximal Promoter ◽  
Specific Expression

Proximal Promoter Sequences of Sunflower Helianthinin Genes Confer Regionalized Seed-Specific Expression

1995 ◽  
Vol 145 (5-6) ◽  
pp. 600-605 ◽  
Author(s):  
Andrew N. Nunberg ◽  
Zhuwen Li ◽  
Hwa-Jee Chung ◽  
Avutu S. Reddy ◽  
Terry L. Thomas
Keyword(s):  
Proximal Promoter ◽  
Specific Expression ◽  
Promoter Sequences ◽  
Seed Specific Expression

scholarly journals Germ cell‐specific expression of Cre recombinase using the VASA promoter in the pig

FEBS Open Bio ◽  
2015 ◽  
Vol 6 (1) ◽  
pp. 50-55 ◽  
Author(s):  
Yuning Song ◽  
Liangxue Lai ◽  
Li Li ◽  
Yongye Huang ◽  
Anfeng Wang ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cre Recombinase ◽  
Specific Expression

Specific expression of a retinoic acid-regulated, zinc-finger gene, Rex-1, in preimplantation embryos, trophoblast and spermatocytes

Development ◽  
1991 ◽  
Vol 113 (3) ◽  
pp. 815-824 ◽  
Author(s):  
M.B. Rogers ◽  
B.A. Hosler ◽  
L.J. Gudas
Keyword(s):  
Retinoic Acid ◽  
Germ Cell ◽  
Cell Fate ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Preimplantation Embryos ◽  
Specific Expression

We have previously isolated a cDNA clone for a gene whose expression is reduced by retinoic acid (RA) treatment of F9 embryonal carcinoma cells. The nucleotide sequence indicated that this gene, Rex-1, encodes a zinc-finger protein and thus may be a transcriptional regulator. The Rex-1 message level is high in two lines of embryonic stem cells (CCE and D3) and is reduced when D3 cells are induced to differentiate using four different growth conditions. As expected for a stem-cell-specific message, Rex-1 mRNA is present in the inner cell mass (ICM) of the day 4.5 mouse blastocyst. It is also present in the polar trophoblast of the blastocyst. One and two days later, Rex-1 message is found in the ectoplacental cone and extraembryonic ectoderm of the egg cylinder (trophoblast-derived tissues), but its abundance is much reduced in the embryonic ectoderm which is directly descended from the ICM. Rex-1 is expressed in the day 18 placenta (murine gestation is 18 days), a tissue which is largely derived from trophoblast. The only tested adult tissue that contains detectable amounts of Rex-1 mRNA is the testis. In situ hybridization and northern analyses of RNA from germ-cell-deficient mouse testis and stage-specific germ cell preparations suggest that Rex-1 expression is limited to spermatocytes (germ cells undergoing meiosis). These results suggest that Rex-1 is involved in trophoblast development and spermatogenesis, and is a useful marker for studies of early cell fate determination in the ICM.

Germ Cell-Specific Expression and Biochemical Characterization of Mouse PAD6.

2008 ◽  
Vol 78 (Suppl_1) ◽  
pp. 297-297
Author(s):  
Keiichiro Yogo ◽  
Grisnarong Wongbandue ◽  
Akihito Ishigami ◽  
Hidenari Takahara ◽  
Tetsuya Kohsaka
Keyword(s):  
Germ Cell ◽  
Biochemical Characterization ◽  
Specific Expression

JG cell expression and partial regulation of a human renin genomic transgene driven by a minimal renin promoter

1999 ◽  
Vol 277 (4) ◽  
pp. F634-F642 ◽  
Author(s):  
Patrick L. Sinn ◽  
Xiaoji Zhang ◽  
Curt D. Sigmund
Keyword(s):  
Gene Expression ◽  
Systemic Circulation ◽  
Proximal Promoter ◽  
Angiotensin Converting Enzyme Inhibition ◽  
Specific Expression ◽  
Renin Synthesis ◽  
Human Renin ◽  
Renin Gene ◽  
Renin Mrna ◽  
Cell Expression

In the kidney, renin gene expression is exquisitely localized to the juxtaglomerular (JG) cells lining the afferent arteriole, having the capacity to regulate renin synthesis in response to a variety of physiological cues. We investigated human renin gene expression in transgenic mice containing a genomic construct driven by 149 bp of its proximal promoter to elucidate whether this was sufficient to confer JG-specific expression. Whereas human renin mRNA was permissively expressed in most tissues, the transgene was expressed mainly in JG cells in the kidney. Active human renin and human prorenin were found in the systemic circulation at levels consistent with previous transgenic models. Remarkably, two lines displayed an appropriate upregulation of transgene mRNA in response to angiotensin-converting enzyme inhibition, and two lines exhibited a downregulation of transgene mRNA in response to subpressor and pressor doses of ANG II. Our results suggest that 149 bp of the human renin proximal promoter, in a context of a genomic construct, are sufficient to confer human renin expression in renal JG cells and at least some aspects of appropriate regulation.

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