Differential regulation of the Oct-3/4 gene in cell culture model systems that parallel different stages of mammalian development

2008 ◽  
Vol 75 (8) ◽  
pp. 1247-1257 ◽  
Author(s):  
Sunil Kumar Mallanna ◽  
Brian Boer ◽  
Michelle Desler ◽  
Angie Rizzino
Keyword(s):  
Cell Culture ◽  
Differential Regulation ◽  
Model Systems ◽  
Mammalian Development ◽  
Cell Culture Model ◽  
Culture Model

Phenolic-enriched fractions from brewers' spent grain possess cellular antioxidant and immunomodulatory effects in cell culture model systems

2013 ◽  
Vol 94 (7) ◽  
pp. 1373-1379 ◽  
Author(s):  
Aoife L McCarthy ◽  
Yvonne C O'Callaghan ◽  
Alan Connolly ◽  
Charles O Piggott ◽  
Richard J FitzGerald ◽  
...  
Keyword(s):  
Cell Culture ◽  
Model Systems ◽  
Cell Culture Model ◽  
Immunomodulatory Effects ◽  
Culture Model ◽  
Spent Grain

scholarly journals Abstract 821: Loss and retention of mutations in cell culture model systems

2017 ◽  
Author(s):  
Pamela S. Shaw ◽  
Rick Nicoletti ◽  
Naghmeh Salimi ◽  
Helen L. Yang ◽  
Abigail E. Witt ◽  
...  
Keyword(s):  
Cell Culture ◽  
Model Systems ◽  
Cell Culture Model ◽  
Culture Model

scholarly journals 3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System

2021 ◽  
Vol 8 (7) ◽  
pp. 97
Author(s):  
Jennifer Rosendahl ◽  
Andreas Svanström ◽  
Mattias Berglin ◽  
Sarunas Petronis ◽  
Yalda Bogestål ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cancer Cell ◽  
Cell Population ◽  
Drug Screening ◽  
Model Systems ◽  
Cancer Drug ◽  
Cell Culture Model ◽  
Culture Model ◽  
3D Printed

Current conventional cancer drug screening models based on two-dimensional (2D) cell culture have several flaws and there is a large need of more in vivo mimicking preclinical drug screening platforms. The microenvironment is crucial for the cells to adapt relevant in vivo characteristics and here we introduce a new cell culture system based on three-dimensional (3D) printed scaffolds using cellulose nanofibrils (CNF) pre-treated with 2,2,6,6-tetramethylpyperidine-1-oxyl (TEMPO) as the structural material component. Breast cancer cell lines, MCF7 and MDA-MB-231, were cultured in 3D TEMPO-CNF scaffolds and were shown by scanning electron microscopy (SEM) and histochemistry to grow in multiple layers as a heterogenous cell population with different morphologies, contrasting 2D cultured mono-layered cells with a morphologically homogenous cell population. Gene expression analysis demonstrated that 3D TEMPO-CNF scaffolds induced elevation of the stemness marker CD44 and the migration markers VIM and SNAI1 in MCF7 cells relative to 2D control. T47D cells confirmed the increased level of the stemness marker CD44 and migration marker VIM which was further supported by increased capacity of holoclone formation for 3D cultured cells. Therefore, TEMPO-CNF was shown to represent a promising material for 3D cell culture model systems for cancer cell applications such as drug screening.

The Effects of Hypokalaemia on the Hormone Exocytosis in Adenohypophysis and Prolactinoma Cell Culture Model Systems

2014 ◽  
Vol 122 (10) ◽  
pp. 575-581 ◽  
Author(s):  
Z. Molnár ◽  
R. Pálföldi ◽  
A. László ◽  
M. Radács ◽  
M. László ◽  
...  
Keyword(s):  
Cell Culture ◽  
Model Systems ◽  
Cell Culture Model ◽  
Culture Model

3D printing novel in vitro cancer cell culture model systems for lung cancer stem cell study

2021 ◽  
Vol 122 ◽  
pp. 111914
Author(s):  
Alejandro Herreros-Pomares ◽  
Xuan Zhou ◽  
Silvia Calabuig-Fariñas ◽  
Se-Jun Lee ◽  
Susana Torres ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Culture ◽  
Stem Cell ◽  
3D Printing ◽  
Cancer Stem Cell ◽  
Model Systems ◽  
Cell Culture Model ◽  
Culture Model ◽  
Cell Study

Hyperphosphorylation of MEF2A in primary adipocytes correlates with downregulation of human GLUT4 gene promoter activity

2007 ◽  
Vol 292 (4) ◽  
pp. E1149-E1156 ◽  
Author(s):  
David P. Sparling ◽  
Beth A. Griesel ◽  
Ann Louise Olson
Keyword(s):  
Cell Culture ◽  
Transcription Factor ◽  
Promoter Activity ◽  
Model Systems ◽  
Regulatory Control ◽  
Cell Culture Model ◽  
In Vitro System ◽  
Culture Model ◽  
Enhancer Factor

GLUT4 promoter activity is regulated by hormonal, metabolic, and tissue-specific controls. This complicates the study of GLUT4 gene transcription, as no cell culture model adequately recapitulates these extracellular regulators. While investigating cultured primary adipocytes as a model system for GLUT4 transcription, we observed that GLUT4 mRNA was specifically and rapidly downregulated upon tissue dispersal. Downregulation of GLUT4 mRNA was mediated in part by loss of regulatory control by the trans-acting factors that control GLUT4 transcriptional activity [the myocyte enhancer factor 2 (MEF2) transcription factor family and the GLUT4 enhancer factor] and their cognate DNA binding sites in transgenic mice. The differences in GLUT4 transcription when whole adipose tissue and cell culture model systems are compared can be correlated to a posttranslational phosphorylation of the transcription factor MEF2A. The difference in the MEF2A phosphorylation state in whole tissue vs. isolated cells may provide a further basis for the development of an in vitro system that could recapitulate fully regulated GLUT4 promoter activity. Development of an in vitro system to reconstitute GLUT4 transcriptional regulation will further efforts to discern the molecular mechanisms that underlie GLUT4 expression.

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