Scrotal heat stress effects on sperm viability, sperm DNA integrity, and the offspring sex ratio in mice

2007 ◽  
Vol 75 (1) ◽  
pp. 40-47 ◽  
Author(s):  
M. Pérez-Crespo ◽  
B. Pintado ◽  
A. Gutiérrez-Adán
Keyword(s):  
Heat Stress ◽  
Sex Ratio ◽  
Dna Integrity ◽  
Sperm Viability ◽  
Offspring Sex Ratio ◽  
Stress Effects ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Offspring Sex

scholarly journals 141 Antioxidant Supplementation Alleviates DNA Damage in Boar Sperm Induced by Tropical Heat Stress

2018 ◽  
Vol 30 (1) ◽  
pp. 210 ◽  
Author(s):  
S. T. Peña ◽  
B. Gummow ◽  
A. J. Parker ◽  
D. B. B. P. Paris
Keyword(s):  
Dna Damage ◽  
Heat Stress ◽  
Dna Integrity ◽  
Antioxidant Supplementation ◽  
Sperm Dna Damage ◽  
Progressive Motility ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Boar Sperm ◽  
The Tropics

Seasonal heat stress is known to significantly diminish reproductive performance in pigs, particularly in the tropics, costing the industry millions in annual losses. The boar’s reduced capacity to sweat and non-pendulous scrotum, combined with the widespread use of European breeds in the tropics, makes this species particularly vulnerable to heat stress. Although heat stress is traditionally considered a sow problem, recent mouse studies demonstrate that heat stress-induced sperm DNA damage can result in arrested development and loss of early embryos. Our study investigated the impact of tropical summer heat stress on the quality and DNA integrity of boar sperm, and trialled antioxidant supplementation to alleviate the problem. Data, expressed as mean ± SEM, were analysed by one-way repeated-measures ANOVA with pairwise Bonferroni tests. Motility of sperm obtained from Large White boars (n = 5) housed in the dry tropics of Townsville, North Queensland, Australia, was characterised by computer-assisted sperm analysis but did not differ between summer, winter, or spring (total motility: 71.3 ± 8.1 v. 90.2 ± 4.2 v. 70.8 ± 5.5%, respectively; P > 0.05; progressive motility: 35.4 ± 7.0 v. 46.6 ± 4.0 v. 41.7 ± 2.8%, respectively; P > 0.05). Sperm DNA integrity in 20,000 sperm/boar per season, evaluated using TUNEL and flow cytometry, revealed 16-fold more DNA-damaged sperm in summer than winter, and nearly 9-fold more than spring (16.1 ± 4.8 v. 1.0 ± 0.2 v. 1.9 ± 0.5%, respectively; P ≤ 0.05). However, boar feed supplemented with 100 g/boar per day of proprietary custom-made antioxidants during summer significantly reduced sperm DNA damage to 9.9 ± 4.5% and 7.2 ± 1.6% (P ≤ 0.05) after 42 and 84 days of treatment respectively. Total and progressive motility were not altered by the supplement. In summary, sperm DNA integrity is compromised in boars during summer, suggesting that boar factors may contribute to seasonal embryo loss in sows. Moreover, such damage appears undetectable using traditional measures of sperm motility. Antioxidant supplementation during summer appears to mitigate the negative impact of heat stress on sperm DNA integrity.

Frequent and mild scrotal heat stress impairs embryo development, implantation and offspring sex ratio in mice

2020 ◽  
Vol 40 (5) ◽  
pp. 617-626 ◽  
Author(s):  
Xiaoyan Wan ◽  
Xiaomei He ◽  
Qian Liu ◽  
Peng Duan ◽  
Honggang Li
Keyword(s):  
Heat Stress ◽  
Sex Ratio ◽  
Embryo Development ◽  
Offspring Sex Ratio ◽  
Offspring Sex

Offspring sex ratio in moose Alces alces in relation to paternal age: an experiment

2004 ◽  
Vol 10 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Bernt-Erik Sæther ◽  
Erling J. Solberg ◽  
Morten Heim ◽  
John E. Stacy ◽  
Kjetill S. Jakobsen ◽  
...  
Keyword(s):  
Sex Ratio ◽  
Alces Alces ◽  
Paternal Age ◽  
Offspring Sex Ratio ◽  
Offspring Sex

scholarly journals Effect of long-term storage in Safe Cell+ extender on boar sperm DNA integrity and other key sperm parameters

2017 ◽  
Vol 59 (1) ◽  
Author(s):  
Wiesław Bielas ◽  
Wojciech Niżański ◽  
Agnieszka Partyka ◽  
Anna Rząsa ◽  
Ryszard Mordak
Keyword(s):  
Sperm Parameters ◽  
Dna Integrity ◽  
Long Term Storage ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Boar Sperm ◽  
Term Storage
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