scholarly journals Characterization of a vasa -like gene from the pacific white shrimp Litopenaeus vannamei and its expression during oogenesis

2006 ◽  
Vol 74 (2) ◽  
pp. 172-177 ◽  
Author(s):  
Eli D. Aflalo ◽  
Anna Bakhrat ◽  
Shaul Raviv ◽  
Daniel Harari ◽  
Amir Sagi ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
The Pacific

Isolation and characterization of two actins of the Pacific white shrimp, Litopenaeus vannamei

2007 ◽  
Vol 151 (6) ◽  
pp. 2145-2151 ◽  
Author(s):  
Piera S. Sun ◽  
Marcus Soderlund ◽  
Nel C. Venzon ◽  
Dailin Ye ◽  
Yuanan Lu
Keyword(s):  
Litopenaeus Vannamei ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Isolation And Characterization ◽  
The Pacific

scholarly journals Biases during DNA extraction affect characterization of the microbiota associated with larvae of the Pacific white shrimp,Litopenaeus vannamei

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5257 ◽  
Author(s):  
Ming Xue ◽  
Liyou Wu ◽  
Yaoyao He ◽  
Huafang Liang ◽  
Chongqing Wen
Keyword(s):  
Dna Extraction ◽  
Litopenaeus Vannamei ◽  
Penaeid Shrimp ◽  
Illumina Miseq ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Dna Recovery ◽  
The Pacific ◽  
Α Diversity

For in-depth characterization of the microbiota associated with shrimp larvae, careful selection of DNA isolation procedure is paramount for avoiding biases introduced in community profiling. Four E.Z.N.A.™ DNA extraction kits, i.e., Bacterial, Mollusc, Stool, and Tissue DNA Kits, abbreviated as Ba, Mo, St, and Ti, respectively, were initially evaluated with zoea 2 (Z2) larvae of the Pacific white shrimp (Litopenaeus vannamei) by 16S amplicon sequencing on a Illumina MiSeq platform. Further characterization of additional larval samples, specifically nauplii 5 (N5), mysis 1 (M1), and postlarvae 1 (P1), was performed with Ba and St kits to examine the changing microbiota profile during shrimp hatchery period. The results from the Z2 samples showed that DNA yields from the four kits varied significantly (P< 0.05), whereas no significant differences were detected in the α-diversity metrics of the microbiota. By contrast, the St kit, with the lowest DNA yield and quality, successfully recovered DNA from Gram-positive and gut-associated bacterial groups, whereas the Ba kit, which showed maximal microbiota similarity with the Mo kit, manifested the best reproducibility. Notably, significant differences were observed in relative abundances of most dominant taxa when comparing results from the Ba and St kits on Z2, M1, and P1 samples. In addition, the bacterial community identified shifted markedly with larval development regardless of the DNA extraction kits. The DNA recovery biases arising from the larval microbiota could be due to different protocols for cell lysis and purification. Therefore, combined application of different DNA extraction methods may facilitate identification of some biologically important groups owing to their complementary effects. This approach should receive adequate attention for a thorough understanding of the larvae-associated microbiota of the penaeid shrimp.

Partial replacement of fish meal with peanut meal in practical diets for the Pacific white shrimp, Litopenaeus vannamei

2011 ◽  
Vol 43 (5) ◽  
pp. 745-755 ◽  
Author(s):  
Xiang-he Liu ◽  
Ji-dan Ye ◽  
Kun Wang ◽  
Jiang-hong Kong ◽  
Wei Yang ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
Fish Meal ◽  
Peanut Meal ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Partial Replacement ◽  
The Pacific
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