scholarly journals Cell cycle analysis and interspecies nuclear transfer of in vitro cultured skin fibroblasts of the Siberian tiger (Panthera tigris Altaica)

2007 ◽  
Vol 74 (4) ◽  
pp. 403-411 ◽  
Author(s):  
Md. Abul Hashem ◽  
Dilip P. Bhandari ◽  
Sung Keun Kang ◽  
Byeong Chun Lee
Keyword(s):  
Cell Cycle ◽  
Nuclear Transfer ◽  
Skin Fibroblasts ◽  
Cell Cycle Analysis ◽  
Panthera Tigris ◽  
Siberian Tiger ◽  
Cultured Skin ◽  
Panthera Tigris Altaica ◽  
Interspecies Nuclear Transfer

Studies of the cell cycle of in vitro cultured skin fibroblasts in goats: work in progress

2003 ◽  
Vol 59 (5-6) ◽  
pp. 1277-1289 ◽  
Author(s):  
Y.S. Yu ◽  
X.S. Sun ◽  
H.N. Jiang ◽  
Y. Han ◽  
C.B. Zhao ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Skin Fibroblasts ◽  
Work In Progress ◽  
Cultured Skin

Cell cycle analysis of in vitro cultured goral (Naemorhedus caudatus) adult skin fibroblasts

2006 ◽  
Vol 30 (9) ◽  
pp. 698-703 ◽  
Author(s):  
M HASHEM ◽  
D BHANDARI ◽  
S KANG ◽  
B LEE ◽  
H SUK
Keyword(s):  
Cell Cycle ◽  
Skin Fibroblasts ◽  
Cell Cycle Analysis ◽  
Adult Skin

Electron Microscopy of Fibroblasts from Myotonic Muscular Dystrophy Patients

1981 ◽  
Vol 39 ◽  
pp. 498-499
Author(s):  
S. E. Miller ◽  
G. B. Hartwig ◽  
R. A. Nielsen ◽  
A. P. Frost ◽  
A. D. Roses
Keyword(s):  
Electron Microscopy ◽  
Muscular Dystrophy ◽  
Genetic Diseases ◽  
Skin Fibroblasts ◽  
Inborn Errors ◽  
Cytoplasmic Inclusions ◽  
Cultured Skin ◽  
Myotonic Muscular Dystrophy ◽  
Glycosaminoglycan Metabolism

Many genetic diseases can be demonstrated in skin cells cultured in vitro from patients with inborn errors of metabolism. Since myotonic muscular dystrophy (MMD) affects many organs other than muscle, it seems likely that this defect also might be expressed in fibroblasts. Detection of an alteration in cultured skin fibroblasts from patients would provide a valuable tool in the study of the disease as it would present a readily accessible and controllable system for examination. Furthermore, fibroblast expression would allow diagnosis of fetal and presumptomatic cases. An unusual staining pattern of MMD cultured skin fibroblasts as seen by light microscopy, namely, an increase in alcianophilia and metachromasia, has been reported; both these techniques suggest an altered glycosaminoglycan metabolism An altered growth pattern has also been described. One reference on cultured skin fibroblasts from a different dystrophy (Duchenne Muscular Dystrophy) reports increased cytoplasmic inclusions seen by electron microscopy. Also, ultrastructural alterations have been reported in muscle and thalamus biopsies from MMD patients, but no electron microscopical data is available on MMD cultured skin fibroblasts.

scholarly journals Cell Cycle Analysis of Chicken Lymphocytes Stimulated to Grow in vitro using PHA and Labelled with BrdU

1989 ◽  
Vol 60 (2) ◽  
pp. 178-184
Author(s):  
Shin OKAMOTO ◽  
Mie OHWAKI ◽  
Yoshizane MAEDA ◽  
Tsutomu HASHIGUCHI
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Analysis

Endoparasites of the Siberian tiger (Panthera tigris altaica )

2018 ◽  
Vol 13 (5) ◽  
pp. 507-516 ◽  
Author(s):  
Tatyana V. MOSKVINA ◽  
Michail Yu. SCHELKANOV ◽  
Mariya A. BEGUN
Keyword(s):  
Panthera Tigris ◽  
Siberian Tiger ◽  
Panthera Tigris Altaica

scholarly journals Hydroxytyrosol Promotes Proliferation of Human Schwann Cells: An In Vitro Study

2020 ◽  
Vol 17 (12) ◽  
pp. 4404
Author(s):  
Khidhir Kamil ◽  
Muhammad Dain Yazid ◽  
Ruszymah Bt Hj Idrus ◽  
Jaya Kumar
Keyword(s):  
Cell Cycle ◽  
Protein Expression ◽  
Schwann Cells ◽  
Growth Factor Receptor ◽  
In Vitro Study ◽  
Cell Cycle Analysis ◽  
Proliferation Index ◽  
Proliferation Markers ◽  
Diphenyltetrazolium Bromide

Recent advances in phytomedicine have explored some potential candidates for nerve regeneration, including hydroxytyrosol (HT). This study was undertaken to explore the potential effects of HT on human Schwann cells’ proliferation. Methods: The primary human Schwann cell (hSC) was characterized, and the proliferation rate of hSC supplemented with various concentrations of HT was determined via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle analysis and protein expression of glial fibrillary acidic protein (GFAP) and p75 nerve growth factor receptor (p75 NGFR) were evaluated via the immunofluorescence technique. Results: In vitro culture of hSCs revealed spindle-like, bipolar morphology with the expression of specific markers of hSC. Hydroxytyrosol at 10 and 20 ng/mL significantly increased the proliferation of hSCs by 30.12 ± 5.9% and 47.8 ± 6.7% compared to control (p < 0.05). Cell cycle analysis showed that HT-treated hSCs have a higher proliferation index (16.2 ± 0.2%) than the control (12.4 ± 0.4%) (p < 0.01). In addition, HT significantly increased the protein expression of GFAP and p75NGFR (p < 0.05). Conclusion: HT stimulates the proliferation of hSCs in vitro, indicated by a significant increase in the hSC proliferation index and protein expression of hSCs’ proliferation markers, namely p75 NGFR and GFAP.

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