Androstenedione increases cytochrome P450 aromatase messenger ribonucleic acid transcripts in nonluteinizing bovine granulosa cells

2004 ◽  
Vol 70 (2) ◽  
pp. 175-183 ◽  
Author(s):  
M�lanie Hamel ◽  
Jens Vanselow ◽  
Edmir S. Nicola ◽  
Christopher A. Price
Keyword(s):  
Cytochrome P450 ◽  
Granulosa Cells ◽  
Ribonucleic Acid ◽  
Messenger Ribonucleic Acid ◽  
P450 Aromatase ◽  
Cytochrome P450 Aromatase

Cytochrome P450 Aromatase Promoter Usage in Bovine Granulosa Cells.

2012 ◽  
Vol 87 (Suppl_1) ◽  
pp. 578-578
Author(s):  
Fatiha Sahmi ◽  
Edmir Nicola ◽  
Gustavo Zamberlam ◽  
Christopher A. Price
Keyword(s):  
Cytochrome P450 ◽  
Granulosa Cells ◽  
P450 Aromatase ◽  
Cytochrome P450 Aromatase ◽  
Promoter Usage

scholarly journals Immunohistochemical localization of cytochrome P450 aromatase in equine gonads.

1995 ◽  
Vol 43 (6) ◽  
pp. 571-577 ◽  
Author(s):  
J Almadhidi ◽  
G E Seralini ◽  
J Fresnel ◽  
P Silberzahn ◽  
J L Gaillard
Keyword(s):  
Cytochrome P450 ◽  
Granulosa Cells ◽  
Immunoblot Analysis ◽  
Immunofluorescent Staining ◽  
Interstitial Tissue ◽  
Experimental Conditions ◽  
Specific Staining ◽  
P450 Aromatase ◽  
Estrogen Synthesis ◽  
Cytochrome P450 Aromatase

Estrogens are the major steroids produced by equine gonads. To identify the cells responsible for estrogen synthesis, an antiserum against purified equine testicular cytochrome P450 aromatase was produced in rabbits. The reactivity and specificity of the antiserum were assessed by ELISA, immunoblot analysis, and immunoneutralization studies. Immunofluorescence microscopy demonstrated that in the male gonad, cytochrome P450 aromatase (P450arom) was localized in the interstitial tissue, whereas, under the experimental conditions used, the Sertoli and germ cells did not show any specific staining. In the ovary, the granulosa cells of small follicles exhibited faint immunofluorescent staining for P450arom and the granulosa cells of large, viable more follicles showed a high degree of immunoreactivity. In the corpus luteum, all the luteinized cells showed immunoreactivity. No immunoreactivity was detected in other cells of small and large viable follicles. Immunolocalization of P450arom in the equine testicular Leydig cells and in ovarian granulosa and luteinized cells indicates that these cells have the ability to metabolize androgens to estrogens and possibly to catechol estrogens.

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