Birth of calves expressing the enhanced green fluorescent protein after transfer of fresh or vitrified/thawed blastocysts produced by somatic cell nuclear transfer

2004 ◽  
Vol 69 (3) ◽  
pp. 278-288 ◽  
Author(s):  
Guochun Gong ◽  
Yunping Dai ◽  
Baoliang Fan ◽  
Huabing Zhu ◽  
Shien Zhu ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent

Expression of Green Fluorescent Protein in Canine-Cloned Embryos Following Interspecies Somatic Cell Nuclear Transfer.

2008 ◽  
Vol 78 (Suppl_1) ◽  
pp. 92-92
Author(s):  
So Gun Hong ◽  
Goo Jang ◽  
Min Kyu Kim ◽  
Hyun Ju Oh ◽  
Jung Eun Park ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Fluorescent Protein ◽  
Green Fluorescent

Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer

2008 ◽  
Vol 53 (7) ◽  
pp. 1035-1039 ◽  
Author(s):  
ZhongHua Liu ◽  
Jun Song ◽  
ZhenKun Wang ◽  
JiangTian Tian ◽  
QingRan Kong ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Fluorescent Protein ◽  
Transgenic Pig ◽  
Green Fluorescent

Production of transgenic porcine blastocysts by hand-made cloning

2004 ◽  
Vol 16 (3) ◽  
pp. 315 ◽  
Author(s):  
P. M. Kragh ◽  
G. Vajta ◽  
T. J. Corydon ◽  
S. Purup ◽  
L. Bolund ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Nuclear Transfer ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Calcium Ionophore ◽  
Calcium Ionophore A23187 ◽  
Ionophore A23187 ◽  
Reconstructed Embryos ◽  
Green Fluorescent

Recently, a zona-free technique for bovine somatic cell nuclear transfer (NT) with no requirement for micromanipulation (i.e. hand-made cloning (HMC)) has been described. The present study demonstrates the application of the HMC technique in the production of transgenic porcine blastocysts. In vitro-matured zona-free porcine oocytes were bisected manually using a microblade and halves containing no chromatin (i.e. the cytoplasts) were selected. Two cytoplasts were electrofused with one transgenic fibroblast expressing enhanced green fluorescent protein and reconstructed embryos were activated in calcium ionophore (A23187) followed by 6-dimethylaminopurine. Subsequently, embryos were cultured in NCSU-23 medium supplemented with 4 mg mL–1 bovine serum albumin for 7 days. In five replicates, 93.0 ± 7.0% (mean ± s.e.m.) of attempted reconstructed embryos fused and survived activation (31/31, 15/23, 28/28, 37/37 and 28/28). On Day 7 after activation, the respective blastocyst rates (per successfully reconstructed embryos) were 6% (2/31), 7% (1/15), 7% (2/28), 3% (1/37) and 7% (2/28), resulting in an average of 6.0 ± 0.8%. Enhanced green fluorescent protein was expressed in all cells of all eight developing blastocysts. Efforts are now directed towards the production of offspring from such transgenic NT blastocysts.

PRODUCTION OF NUCLEAR TRANSFER-DERIVED SWINE THAT EXPRESS THE ENHANCED GREEN FLUORESCENT PROTEIN

2001 ◽  
Vol 12 (2) ◽  
pp. 173-181 ◽  
Author(s):  
Kwang-Wook Park ◽  
Hee-Tae Cheong ◽  
Liangxue Lai ◽  
Gi-Sun Im ◽  
Birgit Kühholzer ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Nuclear Transfer ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent
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