Sexing river buffalo (Bubalus bubalis L.), sheep (Ovis aries L.), goat (Capra hircus L.), and cattle spermatozoa by double color FISH using bovine (Bos taurus L.) X- and Y-painting probes

2003 ◽  
Vol 67 (1) ◽  
pp. 108-115 ◽  
Author(s):  
Dino Di Berardino ◽  
Miluse Vozdova ◽  
Svatava Kubickova ◽  
Halina Cernohorska ◽  
Giuseppe Coppola ◽  
...  
2005 ◽  
Vol 57 (4) ◽  
pp. 494-501 ◽  
Author(s):  
A.R.G.F. Bezerra ◽  
P. Malafaia ◽  
M.C. Mancini ◽  
E.S. Bezerra ◽  
R.A.M. Vieira

Parâmetros cinéticos da degradação ruminal de alguns alimentos utilizados para ruminantes de zoológicos foram estimados mediante incubação in vitro com líquido ruminal de audade (Ammotragus lervia), cervo sambar (Cervus unicolor), elande (Taurotragus oryx), bovino (Bos taurus), bubalino (Bubalus bubalis), caprino (Capra hircus) e ovino (Ovis aries). Os parâmetros cinéticos foram estimados pela técnica da produção de gás, cujos dados foram ajustados pelos modelos de um e de duplo compartimento. Não foram detectadas diferenças nos parâmetros cinéticos que permitissem agrupar os alimentos (fibrosos × não fibrosos) e os animais (domésticos × silvestres). O modelo de duplo compartimento foi o mais adequado para a estimação dos parâmetros cinéticos da degradação ruminal. Inóculo microbiano oriundo de ruminantes domésticos não é recomendado para estimar parâmetros cinéticos da degradação ruminal de alimentos utilizados para ruminantes silvestres de zoológicos.


TecnoLógicas ◽  
2021 ◽  
Vol 24 (50) ◽  
pp. e1731
Author(s):  
Jorge A. Barrero ◽  
Claudia M. Cruz ◽  
Jenny Casallas ◽  
Juan S. Vásquez

La creciente demanda de alimentos de alto valor nutricional ha inclinado el consumo de lácteos de leche bovina por leche proveniente de otras especies de rumiantes; no obstante, la fracción proteica de la leche es relativamente constante y se compone de caseínas: S1-caseína, -caseína, 𝜅-caseína, y seroproteínas; -lactoalbúmina y -lactoglobulina. En la actualidad, los estudios de péptidos bioactivos derivados de la leche se centran en proteínas obtenidas de una única especie y se efectúan empleando enzimas ajenas al sistema digestivo humano. La presente investigación realizó una comparación cuantitativa de los péptidos bioactivos obtenidos de las caseínas y seroproteínas presentes en la leche de bovino (Bos taurus), oveja (Ovis aries), cabra (Capra hircus) y búfalo (Bubalus bubalis) a partir de procesos de digestión in silico catalizados por proteasas presentes en el sistema digestivo humano: pepsina (EC 3.4.23.1), tripsina (EC 3.4.21.4) y quimotripsina (EC 3.4.21.1). La caracterización de péptidos bioactivos y la digestión in silico fue realizada mediante BIOPEP-UMW. La evaluación cuantitativa se efectuó a partir del cálculo de frecuencias de liberación. Los resultados mostraron once clases de péptidos con acción biológica: estimulantes, inhibidores de Renina, inhibidores de DPP4, antioxidantes, inhibidores de ACE, inhibidores de DPP3, hipocolesterolémicos, inhibidores de CaMPDE, reguladores, anticancerígenos e inmunomoduladores. Los péptidos inhibidores de DPP4 presentaron la mayor frecuencia de liberación, lo que sitúa a la leche como una potencial fuente de metabolitos supresores de la acción proteolítica de DPP4 en la degradación de incretinas. Pese a los distintos valores de frecuencias de liberación, los cuatro perfiles totales de péptidos bioactivos correspondientes a la leche de cada especie no mostraron una diferencia estadísticamente significativa (p>0.05).


2008 ◽  
Vol 86 (10) ◽  
pp. 1111-1120 ◽  
Author(s):  
Maryline Pioz ◽  
Anne Loison ◽  
Philippe Gibert ◽  
Jean-Michel Jullien ◽  
Marc Artois ◽  
...  

Microbial infections that affect reproduction are poorly studied in wildlife because reproductive disorders usually go unnoticed. However, such infections are a major concern in domestic ungulates, which raises the question of their influence on reproductive success and population dynamics of wild ungulates. Three bacterial abortive infections are frequent in domestic ruminants: salmonellosis caused by Salmonella enterica serovar Abortusovis, enzootic abortion caused by Chlamydophila abortus , and Q fever caused by Coxiella burnetii . They induce abortions and neonatal mortality in sheep ( Ovis aries L., 1758), goat ( Capra hircus L., 1758), and cattle ( Bos taurus L., 1758). However, little information is known about these bacteria in wild ungulates, particularly in mountain ungulates that may share alpine pastures with infected domestic ruminants. We investigated the relationship between female serological status against Salmonella, Chlamydophila, and Coxiella and her reproductive success. From 1986 to 2003, the reproductive and serological statuses of 125 female chamois ( Rupicapra rupicapra (L., 1758)) aged 2–10 years were available. Reproductive success was the lowest for 2-year-old females and for females born at the highest density. Moreover, we showed for the first time in a wild ungulate population that females with high titer in antibodies against Salmonella experienced a decrease in their reproductive success. Titers in antibodies against Chlamydophila and Coxiella were not related to the reproductive success of female chamois.


Author(s):  
T. Savelyeva ◽  
◽  
М. Shagirbayev ◽  

The article discusses osteological material from the excavations of the Shengeldy caravanserai. In the descriptions of the representatives of the fauna from the medieval caravanserai of Shengeldy and in table 1, the majority of bone remains are identified to a species. Examination of the species composition of the fauna shows their not rich species composition, although as a result of the study all species of domestic ungulates are found. Bone remains were determined by species, skeleton elements and degree of fragmentation. Bones found: horses (Equus caballus), cattle (Bos taurus), sheep (Ovis aries), goats (Capra hircus) and camel (Camelus bactrianus); wild animals - kulan (Equus hemionus), saiga (Saiga tatarica) and turtles (Testudines). Among domestic animals, the bones of horses and small cattle prevail. Cattle ranks third in number. One camel bone was found. Of the bones of domestic animals, 23.4% were intact and 76.6% were fragmented. Among the departments of the skeleton of domestic ungulates, carcass bones (scapula, vertebrae, ribs) and upper legs (femurs and tibia) predominate. Several fragments of human bones were found. Morphometric studies of the bones of horses, sheep, goats and cattle were carried out. Geometric morphometric methods have revealed morphological differences and similarities between species.


2004 ◽  
Vol 67 (1) ◽  
pp. 172-177 ◽  
Author(s):  
MIGUEL A. RODRÍGUEZ ◽  
TERESA GARCÍA ◽  
ISABEL GONZÁLEZ ◽  
LUIS ASENSIO ◽  
PABLO E. HERNÁNDEZ ◽  
...  

A PCR assay has been developed for the specific and qualitative detection of pork ( Sus scrofa domesticus), beef ( Bos taurus), sheep ( Ovis aries), and goat ( Capra hircus) in raw and heat-treated meat mixtures. A forward common primer was designed on a conserved DNA sequence in the mitochondrial 12S ribosomal RNA gene (rRNA), and reverse primers were designed to hybridize on species-specific DNA sequences of each species considered. The different sizes of the species-specific amplicons, separated by agarose gel electrophoresis, allowed clear species identification. Analysis of experimental meat mixtures demonstrated that the detection limit of the assay was 1% (wt/wt) for each species analyzed. This assay can be useful for the accurate identification of these species, avoiding mislabeling or fraudulent species substitution in meat mixtures.


2006 ◽  
Vol 54 (4) ◽  
pp. 1144-1150 ◽  
Author(s):  
Violeta Fajardo ◽  
Isabel González ◽  
Inés López-Calleja ◽  
Irene Martín ◽  
Pablo E. Hernández ◽  
...  

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