Seminal plasma proteins reduce protein tyrosine phosphorylation in the plasma membrane of cold-shocked ram spermatozoa

2002 ◽  
Vol 61 (2) ◽  
pp. 226-233 ◽  
Author(s):  
Rosaura Pérez-Pé ◽  
Patricia Grasa ◽  
Marta Fernández-Juan ◽  
Maria Luisa Peleato ◽  
José Álvaro Cebrián-Pérez ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Tyrosine Phosphorylation ◽  
Seminal Plasma ◽  
Plasma Proteins ◽  
Protein Tyrosine Phosphorylation ◽  
Protein Tyrosine ◽  
Seminal Plasma Proteins

scholarly journals Single Layer Centrifugation Improves the Quality of Fresh Donkey Semen and Modifies the Sperm Ability to Interact with Polymorphonuclear Neutrophils

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2128
Author(s):  
Marion Papas ◽  
Jaime Catalán ◽  
Sandra Recuero ◽  
Jane M. Morrell ◽  
Marc Yeste ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Seminal Plasma ◽  
Plasma Proteins ◽  
Mitochondrial Membrane ◽  
Single Layer ◽  
Polymorphonuclear Neutrophils ◽  
Lipid Disorder ◽  
Seminal Plasma Proteins

This study sought to determine whether single layer centrifugation (SLC) of fresh donkey semen with Equicoll has any impact on sperm quality parameters and on the modulation of endometrial reaction following semen deposition using an in vitro model. Seventeen ejaculates from five jackasses were obtained using an artificial vagina and diluted in a skim-milk extender. Samples were either selected through SLC (Equicoll) or non-treated (control). Two experiments were performed. The first one consisted of incubating selected or non-selected spermatozoa at 38 °C for 180 min. Integrity and lipid disorder of sperm plasma membrane, mitochondrial membrane potential, and intracellular levels of calcium and reactive oxygen species were evaluated at 0, 60, 120, and 180 min. In the second experiment, polymorphonuclear neutrophils (PMN) isolated from jennies blood were mixed with selected and unselected spermatozoa. Interaction between spermatozoa and PMN was evaluated after 0, 60, 120, and 180 min of co-incubation at 38 °C. SLC-selection increased the proportions of spermatozoa with an intact plasma membrane and low lipid disorder, of spermatozoa with high mitochondrial membrane potential and with high calcium levels, and of progressively motile spermatozoa. In addition, selection through SLC augmented the proportion of phagocytosed spermatozoa, which supported the modulating role of seminal plasma proteins on sperm-PMN interaction. In conclusion, SLC of fresh donkey semen increases the proportions of functionally intact and motile spermatozoa, and appears to remove the seminal plasma proteins that inhibit sperm-PMN binding.

scholarly journals Identification of a Subset of Common Variable Immunodeficiency Patients with Impaired B-Cell Protein Tyrosine Phosphorylation

1999 ◽  
Vol 6 (6) ◽  
pp. 856-860 ◽  
Author(s):  
Rivka Schwartz ◽  
Yael Ben-Anat Porat ◽  
Zeev Handzel ◽  
Zeev Sthoeger ◽  
Ben-Zion Garty ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
B Cells ◽  
B Cell ◽  
Tyrosine Phosphorylation ◽  
Common Variable Immunodeficiency ◽  
Cell Protein ◽  
Normal Donor ◽  
Protein Tyrosine Phosphorylation ◽  
Protein Tyrosine ◽  
Common Variable

ABSTRACT The mechanisms responsible for common variable immunodeficiency syndrome (CVID) are as yet unknown. In the present study, we show that the B-cell dysfunction in a subset of CVID patients is caused by defective protein tyrosine phosphorylation (PTP). We demonstrated that the PTP level and immunoglobulin (Ig) secretion malfunctions can be successfully repaired when normal plasma membrane components are implanted into these patients’ B cells. Stimulation of CVID patients’ peripheral blood mononucleated cells with anti-Ig antibody revealed that 7 of 11 patients had lower PTP levels than those found in the normal donor cells. Plasma membrane implantation to the cells of these patients resulted in elevated PTP levels which reached normal levels upon stimulation with anti-human Ig antibody. The results revealed two distinct groups of CVID patients. The first group included patients whose B cells expressed low PTP levels after Ig stimulation. In these patients the plasma membrane implantation restored the normal PTP level as well as the ability to secrete IgM and/or IgG after B-cell stimulation. In the second group, patients whose B cells expressed a normal PTP level after Ig stimulation, with no restoration of their ability to secrete Ig upon plasma membrane implantation and lipopolysaccharide stimulation. We conclude that the first group has an early signal transduction defect located in the B-cell plasma membrane, while in the second group the defect is located elsewhere.

Colloid centrifugation removes seminal plasma and cholesterol from boar spermatozoa

2011 ◽  
Vol 23 (7) ◽  
pp. 858 ◽  
Author(s):  
R. Kruse ◽  
P. C. Dutta ◽  
J. M. Morrell
Keyword(s):  
Plasma Membrane ◽  
Seminal Plasma ◽  
Plasma Proteins ◽  
Single Layer ◽  
Sperm Cryopreservation ◽  
Before And After ◽  
Sperm Plasma Membrane ◽  
Seminal Plasma Proteins ◽  
Boar Spermatozoa ◽  
Layer Chromatography

The objective of the present study was to investigate the effect of Single-Layer Centrifugation (SLC) on boar spermatozoa, namely the effect of removal of seminal plasma proteins and cholesterol from the surface of spermatozoa. The presence of porcine seminal plasma proteins I and II (PSP-I/PSP-II) before and after SLC was studied using immunofluorescence, whereas the removal of cholesterol was shown qualitatively by thin-layer chromatography (TLC). Finally, the integrity of the sperm plasma membrane was observed by electron microscopy. It was shown that the seminal plasma proteins PSP-I and -II were removed from spermatozoa during SLC but could be restored by adding seminal plasma to the SLC-selected sperm samples. Some cholesterol was also lost from the spermatozoa during SLC but the plasma membrane itself appeared to be morphologically intact. Further studies are underway to examine the relevance of these findings to boar sperm cryopreservation and sperm fertility.

Genetic Polymorphism of Boar Seminal Plasma Proteins

1993 ◽  
Vol 64 (3) ◽  
pp. 221-227
Author(s):  
Soichi TSUJI ◽  
Masatoshi ASAO ◽  
Hiroshi KUSUNOKI ◽  
Takao OISHI
Keyword(s):  
Genetic Polymorphism ◽  
Seminal Plasma ◽  
Plasma Proteins ◽  
Seminal Plasma Proteins

Seasonal differences in seminal plasma proteins from two bovine breeds adapted to a subtropical climate

2021 ◽  
Vol 53 (1) ◽  
Author(s):  
Paula Lorena Souto ◽  
Lilian Silveira Travassos Carmouy ◽  
Cristiane Santos ◽  
Edison Martins ◽  
Vera Martins ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Subtropical Climate ◽  
Seasonal Differences ◽  
Seminal Plasma Proteins
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