Solubilization and enrichment of boar sperm membrane proteins

1985 ◽  
Vol 11 (4) ◽  
pp. 335-348 ◽  
Author(s):  
Marianne Klint ◽  
Karin Sege ◽  
Bengt Curman ◽  
Leif Plöen ◽  
Per A. Peterson
Keyword(s):  
Membrane Proteins ◽  
Boar Sperm ◽  
Sperm Membrane

Identification and Localization of Two Boar Sperm-Membrane Proteins

1983 ◽  
pp. 147-150
Author(s):  
Marianne Klint ◽  
Anita Fridberger ◽  
Per A. Peterson ◽  
Leif Plöen
Keyword(s):  
Membrane Proteins ◽  
Boar Sperm ◽  
Sperm Membrane

Partial purification of a boar sperm membrane protein inducing sperm agglutinating antibody

1990 ◽  
Vol 10 (2) ◽  
pp. 131-139
Author(s):  
Oyewole Adeyemo ◽  
E. O. Okegbile ◽  
O. O. Olorunsogo
Keyword(s):  
Molecular Weight ◽  
Membrane Protein ◽  
Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Partial Purification ◽  
Boar Sperm ◽  
Sperm Membrane ◽  
Triton X

For the development of immunological contraception, attention is being concentrated on the possibility of using a sperm membrane antigen. Boar sperm membrane was extracted with triton-X 100 and fractionated by Sephadex G-150 column chromatography. The glycosylated and nonglycosylated portions of protein peaks from the gel filtration were obtained by fractionating on concanavalin A-Sepharose and eluting the bound protein with 0.3 M methyl mannoside. A glycosylated fraction was found to induce sperm agglutinating antibodies in rabbit. The partially purified protein has a molecular weight of 30 kilodaltons, as determined by sodium dodecyl polyaccyrlamide gel electrophoresis. Further work is planned on the histochemical determination of the origin of this protein and species cross-activity of the antibody.

Porcine zona pellucida ZP3? glycoprotein mediates binding of the biotin-labeled Mr 55,000 family (ZP3) to boar sperm membrane vesicles

1993 ◽  
Vol 36 (3) ◽  
pp. 382-389 ◽  
Author(s):  
Edward C. Yurewicz ◽  
Beverley A. Pack ◽  
D. Randall Armant ◽  
Anthony G. Sacco
Keyword(s):  
Zona Pellucida ◽  
Membrane Vesicles ◽  
Boar Sperm ◽  
Sperm Membrane

scholarly journals Seminal plasma arising from the whole boar sperm-rich fraction increases the stability of sperm membrane after thawing1,2

2016 ◽  
Vol 94 (5) ◽  
pp. 1906-1912 ◽  
Author(s):  
M. A. Torres ◽  
G. M. Ravagnani ◽  
D. F. Leal ◽  
S. M. M. K. Martins ◽  
B. B. D. Muro ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Boar Sperm ◽  
Sperm Membrane ◽  
The Stability

80 CHOLESTEROL-LOADED METHYL-BETA-CYCLODEXTRIN IMPROVES BOAR SPERMATOZOA CRYOPROTECTANT ADDITION AND REMOVAL TOLERANCE

2009 ◽  
Vol 21 (1) ◽  
pp. 140
Author(s):  
E. M. Walters ◽  
J. D. Benson ◽  
A. Rieke ◽  
J. K. Graham ◽  
J. K. Critser
Keyword(s):  
Sperm Motility ◽  
Membrane Integrity ◽  
Tolerance Limits ◽  
Permeability Characteristics ◽  
Flow Cytometry Data ◽  
Osmotic Tolerance ◽  
Motile Cells ◽  
Boar Sperm ◽  
Chilling Sensitivity ◽  
Sperm Membrane

Difficulties associated with the cryopreservation of boar sperm include their sensitivities to osmotic stresses and chilling sensitivity. We investigated the effects of cholesterol-loaded cyclodextrin (CLC) on boar sperm motility and membrane integrity following exposure to various osmolalities. Samples were collected using the gloved hand method from crossbred boars, and ejaculates having greater than 75% motility were extended 1:3 with Androhep (Minitube Inc., Verona, WI) for this study. Samples were centrifuged at 700g for 5 min, and the resulting pellets were resuspended to 1.2 × 108 cells mL–1 in Androhep. Samples were then treated with 0, 1.5, or 3.0 mg of CLC/1.2 × 108 cells mL–1 for 10 min at room temperature. In experiment 1, samples were aliquoted into 1.5-mL centrifuge tubes, centrifuged at 700g for 5 min and the sperm exposed to Dulbecco’s PBS at different osmolalities for 5 min before being returned to 300 mOsm by adding Dulbecco’s PBS solutions at differing osmolalities. After returning the sperm to isosmotic conditions, sperm motility was analyzed. In experiment 2, samples were treated as in experiment 1 and following exposure to the various osmolalities, sperm were stained with Alexa 488-PNA and propidium iodide to determine sperm membrane integrity. Ten thousand sperm per treatment were analyzed by flow cytometry. Data were analyzed by standard ANOVA. The CLC-treated sperm (normalized means ± SEM; 33 ± 16, 80 ± 8, 86 ± 5, 100, 64 ± 4, 7 ± 3, 0 ± 0, respectively) exhibited greater percentages of motile cells following hypo-isosmotic exposure than control sperm (4 ± 1.6, 33 ± 9.6, 84 ± 7.1, 100, 37 ± 5.5, 3 ± 1.6, 0 ± 0, respectively), and there was a tendency for CLC-treated sperm (P = 0.0225) to maintain motility following hyper-isosmotic exposure. In addition, CLC-treated sperm (87 ± 4, 93 ± 1, 95 ± 1, 93 ± 2, 88 ± 4, 83 ± 3, 41 ± 9, respectively; P < 0.05) maintained greater percentages of membrane integrity following treatment with anisosmotic solutions compared with controls (29 ± 8, 63 ± 10, 81 ± 7, 92 ± 3, 73 ± 8, 44 ± 5, 21 ± 9, respectively). Using a combination of these osmotic tolerance data with previously published boar sperm membrane permeability characteristics, we mathematically modeled the number of steps needed for the addition or removal of cryoprotectants. Computer simulations indicate that an abrupt addition of 1 m glycerol will cause boar sperm to exceed their osmotic tolerance limits unless they are treated with 3 mg of CLC. Moreover, the addition of 1 m EG causes boar sperm to exceed all osmotic tolerance limits and therefore, the addition and removal of EG requires multiple-step protocols. However, the addition and removal of 1 m DMSO maintains volume excursions well within the osmotic tolerance limits with the addition of cholesterol (1.5 and 3 mg). Empirical data for addition of CPA have shown similar results as seen with the computer simulation. These data support the hypothesis that adding cholesterol to porcine sperm broadens their osmotic tolerance limits and potentially provide a mechanism to increase post-thaw survival of porcine sperm.

Sperm membrane proteins associated with the boar semen cryopreservation

2017 ◽  
Vol 183 ◽  
pp. 27-38 ◽  
Author(s):  
Daianny B. Guimarães ◽  
Tatyane B. Barros ◽  
Maurício F. van Tilburg ◽  
Jorge A.M. Martins ◽  
Arlindo A. Moura ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Semen Cryopreservation ◽  
Sperm Membrane ◽  
Boar Semen

Restricted lateral diffusion of PH-20, a PI-anchored sperm membrane protein

Science ◽  
1988 ◽  
Vol 240 (4860) ◽  
pp. 1780-1782 ◽  
Author(s):  
BM Phelps ◽  
P Primakoff ◽  
DE Koppel ◽  
MG Low ◽  
DG Myles
Keyword(s):  
Membrane Proteins ◽  
Membrane Protein ◽  
Diffusion Rate ◽  
Lateral Diffusion ◽  
Surface Protein ◽  
Integral Membrane Proteins ◽  
Testicular Sperm ◽  
Lateral Mobility ◽  
Sperm Membrane ◽  
Lipid Diffusion

The rate of lateral diffusion of integral membrane proteins is constrained in cells, but the constraining factors for most membrane proteins have not been defined. PH-20, a sperm surface protein involved in sperm-egg adhesion, was shown to be anchored in the plasma membrane by attachment to the lipid phosphatidylinositol and to have a diffusion rate that is highly restricted on testicular sperm, being more than a thousand times slower than lipid diffusion. These results support the hypothesis that lateral mobility of a membrane protein can be regulated exclusively by interactions of its ectodomain.

Effect of staining and sorting on boar sperm membrane integrity, mitochondrial activity and in vitro blastocyst development

2005 ◽  
Vol 64 (1) ◽  
pp. 191-201 ◽  
Author(s):  
M. Spinaci ◽  
M. De Ambrogi ◽  
S. Volpe ◽  
G. Galeati ◽  
C. Tamanini ◽  
...  
Keyword(s):  
Membrane Integrity ◽  
Mitochondrial Activity ◽  
Blastocyst Development ◽  
Boar Sperm ◽  
Sperm Membrane
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