Optimization of culture conditions for the formation of sperm cells in pollen tubes of tradescantia

1981 ◽  
Vol 4 (1) ◽  
pp. 35-40 ◽  
Author(s):  
Gloria J. Lafleur ◽  
Amy E. Gross ◽  
Joseph P. Mascarenhas
Keyword(s):  
Pollen Tubes ◽  
Culture Conditions ◽  
Sperm Cells ◽  
Optimization Of Culture Conditions

scholarly journals Isolation and Optimization of Culture Conditions of Thraustochytrium kinnei for Biomass Production, Nanoparticle Synthesis, Antioxidant and Antimicrobial Activities

2021 ◽  
Vol 9 (6) ◽  
pp. 678
Author(s):  
Kaliyamoorthy Kalidasan ◽  
Nabikhan Asmathunisha ◽  
Venugopal Gomathi ◽  
Laurent Dufossé ◽  
Kandasamy Kathiresan
Keyword(s):  
Antimicrobial Activity ◽  
Gold Nanoparticles ◽  
Silver Nanoparticles ◽  
Biomass Production ◽  
Petroleum Ether Extract ◽  
Antimicrobial Activities ◽  
Culture Conditions ◽  
Gold And Silver Nanoparticles ◽  
Optimization Of Culture Conditions ◽  
Antioxidant And Antimicrobial Activities

This work deals with the identification of a predominant thraustochytrid strain, the optimization of culture conditions, the synthesis of nanoparticles, and the evaluation of antioxidant and antimicrobial activities in biomass extracts and nanoparticles. Thraustochytrium kinnei was identified as a predominant strain from decomposing mangrove leaves, and its culture conditions were optimized for maximum biomass production of 13.53 g·L−1, with total lipids of 41.33% and DHA of 39.16% of total fatty acids. Furthermore, the strain was shown to synthesize gold and silver nanoparticles in the size ranges of 10–85 nm and 5–90 nm, respectively. Silver nanoparticles exhibited higher total antioxidant and DPPH activities than gold nanoparticles and methanol extract of the strain. The silver nanoparticles showed higher antimicrobial activity than gold nanoparticles and petroleum ether extract of the strain. Thus, Thraustochytrium kinnei is proven to be promising for synthesis of silver nanoparticles with high antioxidant and antimicrobial activity.

scholarly journals Persistent directional growth capability in Arabidopsis thaliana pollen tubes after nuclear elimination from the apex

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Kazuki Motomura ◽  
Hidenori Takeuchi ◽  
Michitaka Notaguchi ◽  
Haruna Tsuchi ◽  
Atsushi Takeda ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Pollen Tube ◽  
Sperm Cell ◽  
Pollen Tubes ◽  
Vegetative Nucleus ◽  
Sperm Cells ◽  
Apical Region ◽  
Basal Region ◽  
Specific Expression ◽  
Directional Growth

AbstractDuring the double fertilization process, pollen tubes deliver two sperm cells to an ovule containing the female gametes. In the pollen tube, the vegetative nucleus and sperm cells move together to the apical region where the vegetative nucleus is thought to play a crucial role in controlling the direction and growth of the pollen tube. Here, we report the generation of pollen tubes in Arabidopsis thaliana whose vegetative nucleus and sperm cells are isolated and sealed by callose plugs in the basal region due to apical transport defects induced by mutations in the WPP domain-interacting tail-anchored proteins (WITs) and sperm cell-specific expression of a dominant mutant of the CALLOSE SYNTHASE 3 protein. Through pollen-tube guidance assays, we show that the physiologically anuclear mutant pollen tubes maintain the ability to grow and enter ovules. Our findings provide insight into the sperm cell delivery mechanism and illustrate the independence of the tip-localized vegetative nucleus from directional growth control of the pollen tube.

Optimization of Culture Conditions to Support Long-Term Self-Renewal of Buffalo (Bubalus bubalis) Embryonic Stem Cell-Like Cells

2011 ◽  
Vol 13 (6) ◽  
pp. 539-549 ◽  
Author(s):  
Ruchi Sharma ◽  
Aman George ◽  
Nitin Manchindra Kamble ◽  
Karn Pratap Singh ◽  
Manmohan Singh Chauhan ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Bubalus Bubalis ◽  
Culture Conditions ◽  
Self Renewal ◽  
Optimization Of Culture Conditions
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