Ovoperoxidase activity in ionophore treated mouse eggs. II. Evidence for the enzyme's role in hardening the zona pellucida

1980 ◽  
Vol 3 (3) ◽  
pp. 279-290 ◽  
Author(s):  
Eli D. Schmell ◽  
Bela J. Gulyas
Keyword(s):  
Zona Pellucida ◽  
Treated Mouse ◽  
Mouse Eggs

Viability and growth of mouse embryos after in vitro culture and fusion

Development ◽  
1970 ◽  
Vol 23 (3) ◽  
pp. 693-704
Author(s):  
Patricia Bowman ◽  
Anne McLaren
Keyword(s):  
In Vitro Culture ◽  
Success Rate ◽  
Zona Pellucida ◽  
Growth Regulation ◽  
Excess Mortality ◽  
Blastocyst Stage ◽  
Mouse Embryos ◽  
Foster Mothers ◽  
Mouse Eggs

About 80 % of 8-cell mouse eggs developed to the blastocyst stage in culture, whether the zona pellucida was left intact, or removed with pronase (pre-incubated and dialysed) and the eggs then cultured singly or as fused pairs. When pronase was used without prior incubation and dialysis, the success rate was reduced to 50 %. After transfer to uterine foster-mothers, 20–30 % of apparently normal blastocysts cultured with or without the zona, singly or fused, developed into live foetuses, compared with over 50 % of control blastocysts taken directly from the uterus. Some of the excess mortality of cultured embryos took place before implantation and some soon after. The foetuses derived from cultured blastocysts averaged 0·1 g lighter than those derived from control uterine blastocysts similarly transferred. No differences in the weights of the placentae were observed. Foetal and placental weights were unaffected by whether the eggs had been cultured singly or fused, implying that growth regulation of fused embryos is complete by the 17th day of gestation. The longer the eggs were maintained in culture, the lower was their viability after transfer, and the lighter were the foetuses derived from them.

Rapid, nonradioactive, and quantitative method to analyze zona pellucida modifications in single mouse eggs

1994 ◽  
Vol 38 (1) ◽  
pp. 91-93 ◽  
Author(s):  
Jiri Moos ◽  
Petr Kalab ◽  
Gregory S. Kopf ◽  
Richard M. Schultz
Keyword(s):  
Zona Pellucida ◽  
Quantitative Method ◽  
Mouse Eggs

scholarly journals Murine and human zona pellucida 3 derived from mouse eggs express identical O-glycans

2003 ◽  
Vol 100 (26) ◽  
pp. 15631-15636 ◽  
Author(s):  
A. Dell ◽  
S. Chalabi ◽  
R. L. Easton ◽  
S. M. Haslam ◽  
M. Sutton-Smith ◽  
...  
Keyword(s):  
Zona Pellucida ◽  
Mouse Eggs

scholarly journals Human ZP4 is not sufficient for taxon-specific sperm recognition of the zona pellucida in transgenic mice

Reproduction ◽  
2011 ◽  
Vol 141 (3) ◽  
pp. 313-319 ◽  
Author(s):  
Belinda Yauger ◽  
Nathan A Boggs ◽  
Jurrien Dean
Keyword(s):  
Transgenic Mice ◽  
Zona Pellucida ◽  
Molecular Basis ◽  
Human Sperm ◽  
Protein Isoforms ◽  
Egg Recognition ◽  
Sperm Binding ◽  
Human Fertilization ◽  
Gamete Recognition ◽  
Mouse Eggs

The molecular basis of human fertilization remains enigmatic. Mouse models are often used to study sperm–egg recognition, but the mouse zona pellucida surrounding ovulated eggs contains three proteins (ZP1, ZP2, and ZP3) whereas the human zona contains four (ZP1, ZP2, ZP3, and ZP4). Human sperm are fastidious and recognize human but not mouse eggs. Transgenic mouse lines were established to ascertain whether human ZP4 is the sole determinant of human sperm binding. Human ZP4 expressed in transgenic mice had a molecular mass similar to the range of native protein isoforms and was incorporated into the extracellular zona matrix. Transgenic females were fertile with normal litter sizes. Mouse sperm readily recognized transgenic ovulated eggs, but human sperm did not. We conclude that human ZP4 is not sufficient to support human sperm binding to the zona pellucida in transgenic mice and that other zona proteins may be needed for human gamete recognition.

Transgenic mouse eggs with functional hamster sperm receptors in their zona pellucida

Development ◽  
1992 ◽  
Vol 115 (4) ◽  
pp. 937-946 ◽  
Author(s):  
R.A. Kinloch ◽  
S. Mortillo ◽  
P.M. Wassarman
Keyword(s):  
Transgenic Mouse ◽  
Zona Pellucida ◽  
Transgene Expression ◽  
Acrosome Reaction ◽  
Biologically Active ◽  
Flanking Sequence ◽  
Mouse Oocytes ◽  
Mouse Eggs ◽  
Sperm Receptor

Sperm receptors are located in the mammalian egg extracellular coat, or zona pellucida. Mouse and hamster sperm receptor glycoproteins, mZP3 (83 × 10(3) M(r)) and hZP3 (56 × 10(3) M(r)), respectively, have very similar polypeptides (44 × 10(3) M(r); 81% identical) that are glycosylated to different extents. Purified mZP3 and hZP3 can bind to mouse sperm, prevent them from binding to eggs and induce them to undergo exocytosis, the acrosome reaction, in vitro. A DNA construct that placed the hZP3 gene under the control of mZP3 gene 5′-flanking sequence was used in this report to produce two mouse lines that harbored the foreign sperm receptor transgene. In both lines, the transgene was expressed only by growing oocytes, at a level comparable to that of the endogenous mZP3 gene, and the developmental pattern of transgene expression resembled that of the mZP3 gene. In addition to mZP3, transgenic mouse oocytes synthesized and secreted a glycoprotein indistinguishable from hZP3, and incorporated both glycoproteins into a mosaic zona pellucida. Importantly, hZP3 purified from such zonae pellucidae exhibited both sperm receptor and acrosome reaction-inducing activities in vitro and, following fertilization of transgenic mouse eggs, was inactivated. These results demonstrate that a biologically active foreign sperm receptor can be synthesized and secreted by transgenic mouse oocytes, assembled into a mosaic zona pellucida, and inactivated following fertilization as part of the secondary block to polyspermy.

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