Expression and action of hepatocyte growth factor in bovine endometrial stromal and epithelial cells in vitro

2001 ◽  
Vol 60 (4) ◽  
pp. 472-480 ◽  
Author(s):  
Shuko Murakami ◽  
Yoko Miyamoto ◽  
Chikako Fujiwara ◽  
Sakae Takeuchi ◽  
Sumio Takahashi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Hepatocyte Growth Factor ◽  
Hepatocyte Growth

scholarly journals Scatter factor/hepatocyte growth factor and its receptor, the c-met tyrosine kinase, can mediate a signal exchange between mesenchyme and epithelia during mouse development.

1993 ◽  
Vol 123 (1) ◽  
pp. 223-235 ◽  
Author(s):  
E Sonnenberg ◽  
D Meyer ◽  
K M Weidner ◽  
C Birchmeier
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Hepatocyte Growth Factor ◽  
Tyrosine Kinase ◽  
Cell Surface Receptor ◽  
Mouse Development ◽  
Scatter Factor ◽  
Rnase Protection ◽  
Hepatocyte Growth

Scatter factor/hepatocyte growth factor (SF/HGF) has potent motogenic, mitogenic, and morphogenetic activities on epithelial cells in vitro. The cell surface receptor for this factor was recently identified: it is the product of the c-met protooncogene, a receptor-type tyrosine kinase. We report here the novel and distinct expression patterns of SF/HGF and its receptor during mouse development, which was determined by a combination of in situ hybridization and RNase protection experiments. Predominantly, we detect transcripts of c-met in epithelial cells of various developing organs, whereas the ligand is expressed in distinct mesenchymal cells in close vicinity. In addition, transient SF/HGF and c-met expression is found at certain sites of muscle formation; transient expression of the c-met gene is also detected in developing motoneurons. SF/HGF and the c-met receptor might thus play multiple developmental roles, most notably, mediate a signal given by mesenchyme and received by epithelial. Mesenchymal signals are known to govern differentiation and morphogenesis of many epithelia, but the molecular nature of the signals has remained poorly understood. Therefore, the known biological activities of SF/HGF in vitro and the embryonal expression pattern reported here indicate that this mesenchymal factor can transmit morphogenetic signals in epithelial development and suggest a molecular mechanism for mesenchymal epithelial interactions.

scholarly journals Hepatocyte growth factor is a synergistic factor for the growth of hematopoietic progenitor cells

Blood ◽  
1992 ◽  
Vol 80 (10) ◽  
pp. 2454-2457 ◽  
Author(s):  
TE Kmiecik ◽  
JR Keller ◽  
E Rosen ◽  
GF Vande Woude
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Hepatocyte Growth Factor ◽  
Progenitor Cells ◽  
Messenger Rna ◽  
Hematopoietic Progenitor Cells ◽  
Hematopoietic Progenitor Cell ◽  
Hematopoietic Progenitor ◽  
Hepatocyte Growth

Abstract Bone marrow (BM) stromal cells, which include macrophages, fibroblasts, endothelial cells, and adipocytes, have been shown to produce several factors that modulate the growth of BM progenitors. Hepatocyte growth factor (HGF) is a fibroblast-derived factor and has recently been shown to be a ligand for the c-met proto-oncogene, a member of the receptor class of tyrosine kinases. c-met messenger RNA (mRNA) is predominantly expressed in epithelial cells, but has been detected in several murine hematopoietic progenitor cell lines, suggesting that HGF and met might function during hematopoiesis. Here, BM cells were found to express both met mRNA and protein. Moreover, HGF was shown to synergize with interleukin-3 and granulocyte-macrophage colony-stimulating factor to stimulate colony formation of hematopoietic progenitor cells in vitro. These results show that, in addition to its activity on epithelial cells, HGF is a new member of the functionally related group of factors that modulate hematopoiesis.

scholarly journals Hepatocyte Growth Factor Stimulates Proliferation, Migration, and Lumen Formation of Human Endometrial Epithelial Cells in Vitro

1997 ◽  
Vol 57 (4) ◽  
pp. 936-942 ◽  
Author(s):  
Junichi Sugawara ◽  
Takao Fukaya ◽  
Takashi Murakami ◽  
Hidemune Yoshida ◽  
Akira Yajima
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Hepatocyte Growth Factor ◽  
Lumen Formation ◽  
Hepatocyte Growth ◽  
Endometrial Epithelial Cells

scholarly journals Hepatocyte growth factor is a synergistic factor for the growth of hematopoietic progenitor cells

Blood ◽  
1992 ◽  
Vol 80 (10) ◽  
pp. 2454-2457 ◽  
Author(s):  
TE Kmiecik ◽  
JR Keller ◽  
E Rosen ◽  
GF Vande Woude
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Hepatocyte Growth Factor ◽  
Progenitor Cells ◽  
Messenger Rna ◽  
Hematopoietic Progenitor Cells ◽  
Hematopoietic Progenitor Cell ◽  
Hematopoietic Progenitor ◽  
Hepatocyte Growth

Bone marrow (BM) stromal cells, which include macrophages, fibroblasts, endothelial cells, and adipocytes, have been shown to produce several factors that modulate the growth of BM progenitors. Hepatocyte growth factor (HGF) is a fibroblast-derived factor and has recently been shown to be a ligand for the c-met proto-oncogene, a member of the receptor class of tyrosine kinases. c-met messenger RNA (mRNA) is predominantly expressed in epithelial cells, but has been detected in several murine hematopoietic progenitor cell lines, suggesting that HGF and met might function during hematopoiesis. Here, BM cells were found to express both met mRNA and protein. Moreover, HGF was shown to synergize with interleukin-3 and granulocyte-macrophage colony-stimulating factor to stimulate colony formation of hematopoietic progenitor cells in vitro. These results show that, in addition to its activity on epithelial cells, HGF is a new member of the functionally related group of factors that modulate hematopoiesis.

Hepatocyte growth factor stimulates extensive development of branching duct-like structures by cloned mammary gland epithelial cells

1995 ◽  
Vol 108 (2) ◽  
pp. 413-430 ◽  
Author(s):  
J.V. Soriano ◽  
M.S. Pepper ◽  
T. Nakamura ◽  
L. Orci ◽  
R. Montesano
Keyword(s):  
Mammary Gland ◽  
Growth Factor ◽  
Epithelial Cells ◽  
Hepatocyte Growth Factor ◽  
Conditioned Medium ◽  
Dependent Manner ◽  
Collagen Gels ◽  
Mammary Gland Epithelial Cells ◽  
Hepatocyte Growth

Although epithelial-mesenchymal (stromal) interactions are thought to play an important role in embryonic and postnatal development of the mammary gland, the underlying mechanisms are still poorly understood. To address this issue, we assessed the effect of fibroblast-derived diffusible factors on the growth and morphogenetic properties of a clonally derived subpopulation (clone TAC-2) of normal murine mammary gland (NMuMG) epithelial cells embedded in collagen gels. Under control conditions, TAC-2 mammary gland epithelial cells suspended within collagen gels formed either irregularly shaped cell aggregates or short branching cord-like structures. Addition of conditioned medium from Swiss 3T3 or MRC-5 fibroblasts dramatically stimulated cord formation by TAC-2 cells, resulting in the development of an extensive, highly arborized system of duct-like structures, which in appropriate sections were seen to contain a central lumen. The effect of fibroblast conditioned medium was completely abrogated by antibodies against hepatocyte growth factor (also known as scatter factor), a fibroblast-derived polypeptide that we have previously shown induces tubulogenesis by Madin-Darby canine kidney epithelial cells. Addition of exogenous recombinant human hepatocyte growth factor to collagen gel cultures of TAC-2 cells mimicked the tubulogenic activity of fibroblast conditioned medium by stimulating formation of branching duct-like structures in a dose-dependent manner, with a maximal 77-fold increase in cord length at 20 ng/ml. The effect of either fibroblast conditioned medium or hepatocyte growth factor was markedly potentiated by the simultaneous addition of hydrocortisone (1 microgram/ml), which also enhanced lumen formation. These results demonstrate that hepatocyte growth factor promotes the formation of branching duct-like structures by mammary gland epithelial cells in vitro, and suggest that it may act as a mediator of the inducing effect of mesenchyme (or stroma) on mammary gland development.

scholarly journals Hepatocyte Growth Factor Induces MAPK-Dependent Formin IV Translocation in Renal Epithelial Cells

2000 ◽  
Vol 11 (12) ◽  
pp. 2212-2221
Author(s):  
DAWN A. O'ROURKE ◽  
ZHEN-XIANG LIU ◽  
LORENZ SELLIN ◽  
KATHERINE SPOKES ◽  
ROLF ZELLER ◽  
...  
Keyword(s):  
Growth Factor ◽  
Protein Kinase ◽  
Epithelial Cells ◽  
Hepatocyte Growth Factor ◽  
Mitogen Activated Protein Kinase ◽  
Tubule Formation ◽  
Renal Epithelial Cells ◽  
Mitogen Activated Protein ◽  
Hepatocyte Growth

Abstract. Renal epithelial tubule formation in cultured cells occurs after the addition of tubulogenic growth factors such as the hepatocyte growth factor (HGF). HGF activates the tyrosine kinase receptor c-met, initiating a series of complex events that regulate cell morphology, cell—cell interactions, and cell—matrix interactions and eventually result in the formation of branching tubular structures. The discovery that disruption of the formin gene locus in mice causes agenesis of the kidneys secondary to failure of ureteric bud outgrowth and branching tubule formation suggested that this family of proteins may be critical to the development of renal epithelial tubules. In this study, we investigated whether formin is involved in the HGF/c-met signaling pathway of in vitro tubulogenesis in renal epithelial cells. mIMCD-3 cells were analyzed by reverse transcription-PCR and found to express formin IV mRNA. With the use of an antibody that recognizes the carboxy terminus of all known formin isoforms, it was observed a formin isoform of approximately 165 kD markedly increased in the detergent soluble cell lysate after 10 min of stimulation with HGF. An antibody that is specific for formin IV was then generated and confirmed that the formin isoform regulated by HGF was formin IV. Cell fractionation and confocal localization of formin IV revealed that formin IV is primarily found in a submembranous band that co-localizes with the actin cytoskeleton and in a perinuclear location in quiescent epithelial cells but undergoes a rapid relocalization after HGF stimulation with translocation into the cell cytosol and into the nucleus. Formin IV was found to be a phosphorylation substrate for activated extracellular signal-regulated kinase in vitro, and pretreatment of cells with the mitogen-activated protein kinase inhibitor U0126 prevented the translocation of formin IV and inhibited HGF-dependent phosphorylation of formin IV in intact cells. In conclusion, activation of the c-met receptor results in cellular relocalization of formin IV in a mitogen-activated protein kinase—dependent manner.

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