Epidermal growth factor regulation of connexin 43 in cultured granulosa cells from preantral rabbit follicles

2002 ◽  
Vol 64 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Katie L. Kennedy ◽  
Anthony A. Floyd ◽  
Alison M. Clarkson ◽  
Vaughan H. Lee
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Granulosa Cells ◽  
Connexin 43 ◽  
Epidermal Growth ◽  
Growth Factor Regulation

Molecular Mechanisms of Epidermal Growth Factor Regulation of Prolactin Gene Transcription

1987 ◽  
pp. 473-498
Author(s):  
MICHAEL G. ROSENFELD ◽  
MARIAN L. WATERMAN ◽  
HARRY P. ELSHOLTZ ◽  
HARRY J. MANGALAM ◽  
SCOTT SUPOWIT ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Gene Transcription ◽  
Molecular Mechanisms ◽  
Prolactin Gene ◽  
Epidermal Growth ◽  
Growth Factor Regulation

scholarly journals Epidermal growth factor regulation by autophagy‐mediated lncRNA H19 in murine intestinal tract after severe burn

2020 ◽  
Vol 24 (10) ◽  
pp. 5878-5887
Author(s):  
Cuijie Li ◽  
Mengmeng Zhuang ◽  
Bo Zhu ◽  
Ye Li ◽  
Wenwen Zhang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Intestinal Tract ◽  
Severe Burn ◽  
Epidermal Growth ◽  
Growth Factor Regulation

134. EPIDERMAL GROWTH FACTOR RECEPTOR/MAPK3/1 PATHWAY CROSS-TALK ENABLES GROWTH DIFFERENTIATION FACTOR 9 TO SIGNAL THROUGH SMAD2/3 IN MOUSE GRANULOSA CELLS

2009 ◽  
Vol 21 (9) ◽  
pp. 53
Author(s):  
M. Sasseville ◽  
L. J. Ritter ◽  
T. Nguyen ◽  
D. G. Mottershead ◽  
D. L. Russell ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Granulosa Cells ◽  
Cross Talk ◽  
Egf Receptor ◽  
Thymidine Incorporation ◽  
Differentiation Factor ◽  
Growth Differentiation Factor 9 ◽  
Epidermal Growth ◽  
Receptor Inhibitor

Oocyte-secreted growth differentiation factor 9 (GDF9) plays a critical role throughout folliculogenesis. It has been shown to control many functions of granulosa cells, including gene expression, steroidogenesis and proliferation. This study investigates the cellular requirements that allow GDF9 to act on granulosa cells. Our results showed that GDF9 (20 ng/ml)-stimulated mouse granulosa cells 3H-thymidine incorporation was inhibited by a type 1 receptor Alk4/5/7 inhibitor (SB431542, 5 μM), by an epidermal growth factor (EGF) receptor inhibitor (AG1478, 5μM) and a MEK1 inhibitor (U0126, 10 μM). Interestingly, activin A- and TGFβ-stimulated 3H-thymidine incorporation shared similar inhibitor sensitivity. Moreover, when denuded oocytes were used as the mitogenic agent, SB431542, AG1478 and U0126 all prevented the increase in 3H-thymidine incorporation. Oocyte-stimulated 3H-thymidine incorporation in secondary follicles and cumulus-oocyte complexes were also sensitive to Alk4/5/7, EGF receptor and MEK1 inhibition. Basal and EGF-stimulated levels of phopho-MAPK3/1 were inhibited by using the EGF receptor inhibitor, but were not affected by inhibition of Alk4/5/7 or by adding GDF9 in granulosa cells. Using granulosa cells transfected with a SMAD3-luciferase reporter construct, GDF9-stimulated SMAD3 response could be inhibited by Alk4/5/7, EGFR and MEK1 inhibitors. Genes involved in cumulus cells expansion (Ptx3 and Has2) were upregulated in granulosa cells by co-culturing with denuded oocytes and that upregulation was inhibited by Alk4/5/7 as well as by EGF receptor inhibition. These results suggest that TGFβ superfamily members signalling through Smad2/3 share a common requirement of EGF receptor-dependant phospho-MAPK3/1 throughout folliculogenesis. These results strongly suggest that, apart from its role in the transmission of the ovulatory LH signal within the ovarian follicle, EGF receptor pathway might serve as modulators of GDF9 action on granulosa cells. Hence the interaction between endocrine and oocyte signalling may be mediated at the level of MAPK and Smad2/3 cross-talk in granulosa cells.

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