Stage of the estrous cycle at the time of pregnant mare's serum gonadotropin injection affects pre-implantation embryo development in vitro in the mouse

2002 ◽  
Vol 62 (3) ◽  
pp. 312-319 ◽  
Author(s):  
Juan J. Tarín ◽  
Sonia Pérez-Albalá ◽  
Vanessa Gómez-Piquer ◽  
Carlos Hermenegildo ◽  
Antonio Cano
Keyword(s):  
Estrous Cycle ◽  
Embryo Development ◽  
Serum Gonadotropin ◽  
Development In Vitro

scholarly journals Analysis of Morphokinetic Parameters of Feline Embryos Using a Time-Lapse System

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 748
Author(s):  
Joanna Kochan ◽  
Agnieszka Nowak ◽  
Barbara Kij ◽  
Sylwia Prochowska ◽  
Wojciech Niżański
Keyword(s):  
Embryo Development ◽  
Embryo Quality ◽  
Time Lapse ◽  
Blastocyst Stage ◽  
Cavity Formation ◽  
Non Invasive ◽  
Morphokinetic Parameters ◽  
Morphological Defects ◽  
Development In Vitro

The aim of this study was to analyze the morphokinetic parameters of feline embryos using a time lapse system. Oocytes matured in vitro were fertilized (IVF) and in vitro cultured in a time lapse-system (Primo Vision®, Gothenburg, Sweden). The first cell division of embryos occurred between 17 h post insemination (hpi) and 38 hpi, with the highest proportion of embryos (46%) cleaving between 21 and 24 hpi. The timing of the first cleavage significantly affected further embryo development, with the highest development occurring in embryos that cleaved at 21–22 hpi. Embryos that cleaved very early (17–18 hpi) developed poorly to the blastocyst stage (2%) and none of the embryos that cleaved later than 27 hpi were able to reach the blastocyst stage. Morphological defects were observed in 48% of the embryos. There were no statistically significant differences between the timing intervals of the first cleavage division and the frequency of morphological defects in embryos. Multiple (MUL) morphological defects were detected in more than half (56%) of the abnormal embryos. The most frequent single morphological defects were cytoplasmic fragmentation (FR) (8%) and blastomere asymmetry (AS) (6%). Direct cleavage (DC) from 1–3 or 3–5 blastomeres, reverse cleavage (RC) and vacuoles were rarely observed (2–3%). The timing of blastocyst cavity formation is a very good indicator of embryo quality. In our study, blastocyst cavity formation occurred between 127–167 hpi, with the highest frequency of hatching observed in blastocysts that cavitated between 142–150 hpi. Blastocysts in which cavitation began after 161 h did not hatch. In conclusion, the timing of the first and second cleavage divisions, the timing of blastocyst cavity formation and morphological anomalies can all be used as early and non-invasive indicators of cat embryo development in vitro.

Comparative effects of essential and nonessential metals on preimplantation mouse embryo development in vitro

Toxicology ◽  
1997 ◽  
Vol 116 (1-3) ◽  
pp. 123-131 ◽  
Author(s):  
Lynn A. Hanna ◽  
Jeffrey M. Peters ◽  
Lynn M. Wiley ◽  
Michael S. Clegg ◽  
Carl L. Keen
Keyword(s):  
Embryo Development ◽  
Mouse Embryo ◽  
Mouse Embryo Development ◽  
Preimplantation Mouse Embryo ◽  
Preimplantation Mouse ◽  
Development In Vitro

scholarly journals Regulation of Hamster Embryo Development in Vitro by Carbon Dioxide1

1987 ◽  
Vol 36 (5) ◽  
pp. 1155-1163 ◽  
Author(s):  
E. W. Carney ◽  
B. D. Bavister
Keyword(s):  
Embryo Development ◽  
Development In Vitro

The effect of perfluorohexanesulfonate (PFHxS) on bovine preimplantation embryo development in vitro

2021 ◽  
Vol 350 ◽  
pp. S169-S170
Author(s):  
I. Hallberg ◽  
M. Moberg ◽  
M. Olovsson ◽  
P. Damdimopoulou ◽  
J. Rüegg ◽  
...  
Keyword(s):  
Embryo Development ◽  
Preimplantation Embryo ◽  
Preimplantation Embryo Development ◽  
Development In Vitro

Fibroblasts from the new-born male testicle of Guangxi Bama mini-pig (Sus scrofa) can support nuclear transferred embryo development in vitro – CORRIGENDUM

Zygote ◽  
2009 ◽  
Vol 17 (2) ◽  
pp. 185-185
Author(s):  
Hong-Bo Liu ◽  
Pei-Ru Lv ◽  
Xiao-Gan Yang ◽  
Xiao-E Qin ◽  
Dao-Yuan Pi ◽  
...  
Keyword(s):  
Embryo Development ◽  
Sus Scrofa ◽  
Title Page ◽  
New Born ◽  
Development In Vitro

The authors apologise for the wrong affiliations being assigned to some of the authors, given on the title page of their paper (Liu, et al., 2009) published in Zygote.

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